Mutational Analysis of Saccharomyces cerevisiae ARF1(∗)

Wild type and eight point mutants of Saccharomyces cerevisiae ARF1 were expressed in yeast and bacteria to determine the roles of specific residues in in vivo and in vitro activities. Mutations at either Gly2 or Asp26 resulted in recessive loss of function. It was concluded that N-myristoylation is...

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Veröffentlicht in:	The Journal of biological chemistry 1995-01, Vol.270 (1), p.143-150
Hauptverfasser:	Kahn, Richard A., Clark, Jenny, Rulka, Cherrie, Stearns, Tim, Zhang, Chun-jiang, Randazzo, Paul A., Terui, Takeshi, Cavenagh, Margaret
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE ASPARTIQUE ACIDO ASPARTICO ADP-Ribosylation Factor 1 ADP-Ribosylation Factors Alleles Amino Acid Sequence Aspartic Acid - genetics Cysteine - genetics DNA Mutational Analysis GENE GENES Genes, Dominant Genes, Lethal GLICINA GLYCINE (ACIDE AMINE) GTP-Binding Proteins - genetics GTP-Binding Proteins - metabolism Guanosine Triphosphate - metabolism Molecular Sequence Data MUTACION INDUCIDA MUTANT MUTANTES MUTATION PROVOQUEE NUCLEOTIDE NUCLEOTIDOS Point Mutation Protein Binding SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - metabolism
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