Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey
Antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and HPB carrying ARGs pose a high risk to soil ecology and public health. Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and...
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| Veröffentlicht in: | Environmental science & technology 2015-01, Vol.49 (2), p.1095-1104 |
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| creator | Fang, Hua Wang, Huifang Cai, Lin Yu, Yunlong |
| description | Antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and HPB carrying ARGs pose a high risk to soil ecology and public health. Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and Hushu vegetable bases with different greenhouse planting years in Nanjing, Eastern China. There was a positive correlation between the levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in manures and greenhouse soils. In total, 156.2–5001.4 μg/kg of antibiotic residues, 22 classes of ARGs, 32 HPB species, and 46 species of HPB carrying ARGs were found. The highest relative abundance was tetracycline resistance genes (manures) and multidrug resistance genes (greenhouse soils). The dominant HPB and HPB carrying ARGs in the manures were Bacillus anthracis, Bordetella pertussis, and B. anthracis (sulfonamide resistance gene, sul1), respectively. The corresponding findings in greenhouse soils were Mycobacterium tuberculosis and M. ulcerans, M. tuberculosis (macrolide-lincosamide-streptogramin resistance protein, MLSRP), and B. anthracis (sul1), respectively. Our findings confirmed high levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in the manured greenhouse soils compared with those in the field soils, and their relative abundance increased with the extension of greenhouse planting years. |
| doi_str_mv | 10.1021/es504157v |
| format | Article |
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Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and Hushu vegetable bases with different greenhouse planting years in Nanjing, Eastern China. There was a positive correlation between the levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in manures and greenhouse soils. In total, 156.2–5001.4 μg/kg of antibiotic residues, 22 classes of ARGs, 32 HPB species, and 46 species of HPB carrying ARGs were found. The highest relative abundance was tetracycline resistance genes (manures) and multidrug resistance genes (greenhouse soils). The dominant HPB and HPB carrying ARGs in the manures were Bacillus anthracis, Bordetella pertussis, and B. anthracis (sulfonamide resistance gene, sul1), respectively. The corresponding findings in greenhouse soils were Mycobacterium tuberculosis and M. ulcerans, M. tuberculosis (macrolide-lincosamide-streptogramin resistance protein, MLSRP), and B. anthracis (sul1), respectively. Our findings confirmed high levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in the manured greenhouse soils compared with those in the field soils, and their relative abundance increased with the extension of greenhouse planting years.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es504157v</identifier><identifier>PMID: 25514174</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Antibiotics ; Bacillus anthracis ; Bacteria ; Bacteria - genetics ; Bordetella pertussis ; Chickens ; China ; Correlation analysis ; DNA - analysis ; Drug resistance ; Drug Resistance, Microbial - genetics ; Genes ; Genes, Bacterial ; Greenhouses ; Manure - microbiology ; Manures ; Metagenomics ; Mycobacterium tuberculosis ; Risk assessment ; RNA, Ribosomal, 16S - analysis ; Soil ; Soil Microbiology ; Tetracycline Resistance - genetics ; Tuberculosis</subject><ispartof>Environmental science & technology, 2015-01, Vol.49 (2), p.1095-1104</ispartof><rights>Copyright American Chemical Society Jan 20, 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-9b63d6912d4a8b0f846f40852081c7c8da06f6383a6c02b3ccbb54beeb674a8c3</citedby><cites>FETCH-LOGICAL-a442t-9b63d6912d4a8b0f846f40852081c7c8da06f6383a6c02b3ccbb54beeb674a8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es504157v$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es504157v$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25514174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fang, Hua</creatorcontrib><creatorcontrib>Wang, Huifang</creatorcontrib><creatorcontrib>Cai, Lin</creatorcontrib><creatorcontrib>Yu, Yunlong</creatorcontrib><title>Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and HPB carrying ARGs pose a high risk to soil ecology and public health. Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and Hushu vegetable bases with different greenhouse planting years in Nanjing, Eastern China. There was a positive correlation between the levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in manures and greenhouse soils. In total, 156.2–5001.4 μg/kg of antibiotic residues, 22 classes of ARGs, 32 HPB species, and 46 species of HPB carrying ARGs were found. The highest relative abundance was tetracycline resistance genes (manures) and multidrug resistance genes (greenhouse soils). The dominant HPB and HPB carrying ARGs in the manures were Bacillus anthracis, Bordetella pertussis, and B. anthracis (sulfonamide resistance gene, sul1), respectively. The corresponding findings in greenhouse soils were Mycobacterium tuberculosis and M. ulcerans, M. tuberculosis (macrolide-lincosamide-streptogramin resistance protein, MLSRP), and B. anthracis (sul1), respectively. Our findings confirmed high levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in the manured greenhouse soils compared with those in the field soils, and their relative abundance increased with the extension of greenhouse planting years.</description><subject>Animals</subject><subject>Antibiotics</subject><subject>Bacillus anthracis</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bordetella pertussis</subject><subject>Chickens</subject><subject>China</subject><subject>Correlation analysis</subject><subject>DNA - analysis</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Greenhouses</subject><subject>Manure - microbiology</subject><subject>Manures</subject><subject>Metagenomics</subject><subject>Mycobacterium tuberculosis</subject><subject>Risk assessment</subject><subject>RNA, Ribosomal, 16S - analysis</subject><subject>Soil</subject><subject>Soil Microbiology</subject><subject>Tetracycline Resistance - genetics</subject><subject>Tuberculosis</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkV9LHDEUxUOp1K32oV-gBIpQH8bm_2QfV2nXwkpFLfg2JJk7GplJNJlZ2I_Qb93IWhF9ug_3d865l4PQZ0qOKGH0O2RJBJX1-h2aUclIJbWk79GMEMqrOVfXu-hjzneEEMaJ_oB2mZRU0FrM0N_zBGvTQ3CAY4cXYfTWx9E7fAHZ59E8LpYQIGMTWnxs3AjJmx6fm_E23kDI2Ae8iuGmuoI04DMTpgQtXiaAcBunDPgy-j7jRS6OayhRLbYbfAajKeo4lKTLKa1hs492OtNn-PQ099Cfnz-uTk6r1e_lr5PFqjJCsLGaW8VbNaesFUZb0mmhOkF0-VpTVzvdGqI6xTU3yhFmuXPWSmEBrKqLwvE99G3re5_iwwR5bAafHfS9CVDubahShGtVz1lBv75C7-KUQrmuUEJzzZisC3W4pVyKOSfomvvkB5M2DSXNYz_Ncz-F_fLkONkB2mfyfyEFONgCxuUXaW-M_gGVkZd6</recordid><startdate>20150120</startdate><enddate>20150120</enddate><creator>Fang, Hua</creator><creator>Wang, Huifang</creator><creator>Cai, Lin</creator><creator>Yu, Yunlong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7QL</scope><scope>7T2</scope><scope>7U1</scope><scope>7U2</scope><scope>RC3</scope></search><sort><creationdate>20150120</creationdate><title>Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey</title><author>Fang, Hua ; Wang, Huifang ; Cai, Lin ; Yu, Yunlong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-9b63d6912d4a8b0f846f40852081c7c8da06f6383a6c02b3ccbb54beeb674a8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antibiotics</topic><topic>Bacillus anthracis</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bordetella pertussis</topic><topic>Chickens</topic><topic>China</topic><topic>Correlation analysis</topic><topic>DNA - analysis</topic><topic>Drug resistance</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Greenhouses</topic><topic>Manure - microbiology</topic><topic>Manures</topic><topic>Metagenomics</topic><topic>Mycobacterium tuberculosis</topic><topic>Risk assessment</topic><topic>RNA, Ribosomal, 16S - analysis</topic><topic>Soil</topic><topic>Soil Microbiology</topic><topic>Tetracycline Resistance - genetics</topic><topic>Tuberculosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Hua</creatorcontrib><creatorcontrib>Wang, Huifang</creatorcontrib><creatorcontrib>Cai, Lin</creatorcontrib><creatorcontrib>Yu, Yunlong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Risk Abstracts</collection><collection>Safety Science and Risk</collection><collection>Genetics Abstracts</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Hua</au><au>Wang, Huifang</au><au>Cai, Lin</au><au>Yu, Yunlong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2015-01-20</date><risdate>2015</risdate><volume>49</volume><issue>2</issue><spage>1095</spage><epage>1104</epage><pages>1095-1104</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and HPB carrying ARGs pose a high risk to soil ecology and public health. Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and Hushu vegetable bases with different greenhouse planting years in Nanjing, Eastern China. There was a positive correlation between the levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in manures and greenhouse soils. In total, 156.2–5001.4 μg/kg of antibiotic residues, 22 classes of ARGs, 32 HPB species, and 46 species of HPB carrying ARGs were found. The highest relative abundance was tetracycline resistance genes (manures) and multidrug resistance genes (greenhouse soils). The dominant HPB and HPB carrying ARGs in the manures were Bacillus anthracis, Bordetella pertussis, and B. anthracis (sulfonamide resistance gene, sul1), respectively. The corresponding findings in greenhouse soils were Mycobacterium tuberculosis and M. ulcerans, M. tuberculosis (macrolide-lincosamide-streptogramin resistance protein, MLSRP), and B. anthracis (sul1), respectively. Our findings confirmed high levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in the manured greenhouse soils compared with those in the field soils, and their relative abundance increased with the extension of greenhouse planting years.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25514174</pmid><doi>10.1021/es504157v</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Antibiotics Bacillus anthracis Bacteria Bacteria - genetics Bordetella pertussis Chickens China Correlation analysis DNA - analysis Drug resistance Drug Resistance, Microbial - genetics Genes Genes, Bacterial Greenhouses Manure - microbiology Manures Metagenomics Mycobacterium tuberculosis Risk assessment RNA, Ribosomal, 16S - analysis Soil Soil Microbiology Tetracycline Resistance - genetics Tuberculosis |
| title | Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey |
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