Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus
The growth hormone secretagogue-receptor (GHS-R) is an endogenous receptor for the gut hormone ghrelin. Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a p...
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| Veröffentlicht in: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2015-01, Vol.179, p.54-61 |
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| container_title | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology |
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| creator | Cai, Wen-jing Yuan, Xiao-chen Yuan, Yong-chao Xie, Shou-qi Gong, Yuan Su, Hang Qiao, Yang |
| description | The growth hormone secretagogue-receptor (GHS-R) is an endogenous receptor for the gut hormone ghrelin. Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a peptide of 360 amino acid residues. Comparison analysis revealed that the amino acid sequences of GHS-R1a were highly conserved in vertebrates and shared 97% amino acid identity with zebrafish (Danio rerio), 96% with jian carp (Cyprinus carpio var. Jian) and 93% with goldfish (Carassius auratus). The GHS-R1a showed the highest level of mRNA expression in the pituitary, followed by the brain and liver, and the lowest expression was observed in the hindgut. Intraperitoneally injected with grass carp ghrelin (50, 100 and 150ng/g body weight (BW)), grass carp showed greater mRNA expression of GHS-R1a in the pituitary compared with saline injected at 0.5h postinjection. It was observed that food deprivation could promote the expression of ghrelin and GHS-R1a in the pituitary, demonstrating that nutritional status can influence the expression of both ghrelin and GHS-R1a in the pituitary. After a 2- or 4-week fast, plasma growth hormone (GH) increased, was positively correlated with ghrelin and GHS-R1a mRNA expression levels in the pituitary. These results suggested that the involvement of ghrelin/GHS-R1a systems in mediating the effects of nutritional status and ghrelin on growth processes in grass carp. |
| doi_str_mv | 10.1016/j.cbpa.2014.09.009 |
| format | Article |
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Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a peptide of 360 amino acid residues. Comparison analysis revealed that the amino acid sequences of GHS-R1a were highly conserved in vertebrates and shared 97% amino acid identity with zebrafish (Danio rerio), 96% with jian carp (Cyprinus carpio var. Jian) and 93% with goldfish (Carassius auratus). The GHS-R1a showed the highest level of mRNA expression in the pituitary, followed by the brain and liver, and the lowest expression was observed in the hindgut. Intraperitoneally injected with grass carp ghrelin (50, 100 and 150ng/g body weight (BW)), grass carp showed greater mRNA expression of GHS-R1a in the pituitary compared with saline injected at 0.5h postinjection. It was observed that food deprivation could promote the expression of ghrelin and GHS-R1a in the pituitary, demonstrating that nutritional status can influence the expression of both ghrelin and GHS-R1a in the pituitary. After a 2- or 4-week fast, plasma growth hormone (GH) increased, was positively correlated with ghrelin and GHS-R1a mRNA expression levels in the pituitary. These results suggested that the involvement of ghrelin/GHS-R1a systems in mediating the effects of nutritional status and ghrelin on growth processes in grass carp.</description><identifier>ISSN: 1095-6433</identifier><identifier>EISSN: 1531-4332</identifier><identifier>DOI: 10.1016/j.cbpa.2014.09.009</identifier><identifier>PMID: 25242546</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Carassius auratus ; Carps - blood ; Carps - genetics ; cDNA cloning ; Cloning, Molecular ; Ctenopharyngodon idella ; Cyprinus carpio ; Danio rerio ; Energy homeostasis ; Fasting ; Food Deprivation ; Gene Expression Profiling ; Gene Expression Regulation ; Genome ; Ghrelin ; Ghrelin - administration & dosage ; Ghrelin - genetics ; Ghrelin - metabolism ; GHS-R ; Grass carp ; Growth Hormone - blood ; Molecular Sequence Data ; Organ Specificity - genetics ; Phylogeny ; Pituitary Gland - metabolism ; Real-Time Polymerase Chain Reaction ; Receptors, Ghrelin - chemistry ; Receptors, Ghrelin - genetics ; Receptors, Ghrelin - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Analysis, DNA</subject><ispartof>Comparative biochemistry and physiology. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-95a0cd70fc0e0aef085257c4e10445e78b12aebb9f0a4f4d278254127221267c3</citedby><cites>FETCH-LOGICAL-c389t-95a0cd70fc0e0aef085257c4e10445e78b12aebb9f0a4f4d278254127221267c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cbpa.2014.09.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25242546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Wen-jing</creatorcontrib><creatorcontrib>Yuan, Xiao-chen</creatorcontrib><creatorcontrib>Yuan, Yong-chao</creatorcontrib><creatorcontrib>Xie, Shou-qi</creatorcontrib><creatorcontrib>Gong, Yuan</creatorcontrib><creatorcontrib>Su, Hang</creatorcontrib><creatorcontrib>Qiao, Yang</creatorcontrib><title>Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus</title><title>Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</title><addtitle>Comp Biochem Physiol A Mol Integr Physiol</addtitle><description>The growth hormone secretagogue-receptor (GHS-R) is an endogenous receptor for the gut hormone ghrelin. Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a peptide of 360 amino acid residues. Comparison analysis revealed that the amino acid sequences of GHS-R1a were highly conserved in vertebrates and shared 97% amino acid identity with zebrafish (Danio rerio), 96% with jian carp (Cyprinus carpio var. Jian) and 93% with goldfish (Carassius auratus). The GHS-R1a showed the highest level of mRNA expression in the pituitary, followed by the brain and liver, and the lowest expression was observed in the hindgut. Intraperitoneally injected with grass carp ghrelin (50, 100 and 150ng/g body weight (BW)), grass carp showed greater mRNA expression of GHS-R1a in the pituitary compared with saline injected at 0.5h postinjection. It was observed that food deprivation could promote the expression of ghrelin and GHS-R1a in the pituitary, demonstrating that nutritional status can influence the expression of both ghrelin and GHS-R1a in the pituitary. After a 2- or 4-week fast, plasma growth hormone (GH) increased, was positively correlated with ghrelin and GHS-R1a mRNA expression levels in the pituitary. These results suggested that the involvement of ghrelin/GHS-R1a systems in mediating the effects of nutritional status and ghrelin on growth processes in grass carp.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Carassius auratus</subject><subject>Carps - blood</subject><subject>Carps - genetics</subject><subject>cDNA cloning</subject><subject>Cloning, Molecular</subject><subject>Ctenopharyngodon idella</subject><subject>Cyprinus carpio</subject><subject>Danio rerio</subject><subject>Energy homeostasis</subject><subject>Fasting</subject><subject>Food Deprivation</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genome</subject><subject>Ghrelin</subject><subject>Ghrelin - administration & dosage</subject><subject>Ghrelin - genetics</subject><subject>Ghrelin - metabolism</subject><subject>GHS-R</subject><subject>Grass carp</subject><subject>Growth Hormone - blood</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity - genetics</subject><subject>Phylogeny</subject><subject>Pituitary Gland - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Ghrelin - chemistry</subject><subject>Receptors, Ghrelin - genetics</subject><subject>Receptors, Ghrelin - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><issn>1095-6433</issn><issn>1531-4332</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1P3DAUtCpQobR_oAfkI4dNeHbsfEi9VCsolZA4QM-W47wEr3bj1M4iyq_nRQs9VvXF70kzozczjH0VkAsQ5eUmd-1kcwlC5dDkAM0Hdip0ITJVFPKIZmh0VtJywj6ltAF6SqiP7ERqqaRW5Snz9_h7j6PDFR9wDDvveIiDHf2LnX0YuR07js9TxJSWNfR8eIy49SOP6HCaQ-Q0D9GmxJ2N04qvZ9KZHm38Mw6hI47vcLvdp8_suLfbhF_e_jP26_rqYX2T3d79-Ln-fpu5om7mrNEWXFdB7wDBYg-1lrpyCgUopbGqWyEttm3Tg1W96mRVkxMhKymFLCtXnLGLg-4UA1lLs9n55OgEO2LYJyPKEoq61LX6D6hsdE1ATVB5gLoYUorYmyn6HZk0AszShtmYpQ2ztGGgMdQGkc7f9PftDru_lPf4CfDtAEAK5MljNMn5pY3OU7yz6YL_l_4rtR2bvg</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Cai, Wen-jing</creator><creator>Yuan, Xiao-chen</creator><creator>Yuan, Yong-chao</creator><creator>Xie, Shou-qi</creator><creator>Gong, Yuan</creator><creator>Su, Hang</creator><creator>Qiao, Yang</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201501</creationdate><title>Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus</title><author>Cai, Wen-jing ; Yuan, Xiao-chen ; Yuan, Yong-chao ; Xie, Shou-qi ; Gong, Yuan ; Su, Hang ; Qiao, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-95a0cd70fc0e0aef085257c4e10445e78b12aebb9f0a4f4d278254127221267c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Carassius auratus</topic><topic>Carps - blood</topic><topic>Carps - genetics</topic><topic>cDNA cloning</topic><topic>Cloning, Molecular</topic><topic>Ctenopharyngodon idella</topic><topic>Cyprinus carpio</topic><topic>Danio rerio</topic><topic>Energy homeostasis</topic><topic>Fasting</topic><topic>Food Deprivation</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genome</topic><topic>Ghrelin</topic><topic>Ghrelin - administration & dosage</topic><topic>Ghrelin - genetics</topic><topic>Ghrelin - metabolism</topic><topic>GHS-R</topic><topic>Grass carp</topic><topic>Growth Hormone - blood</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity - genetics</topic><topic>Phylogeny</topic><topic>Pituitary Gland - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Ghrelin - chemistry</topic><topic>Receptors, Ghrelin - genetics</topic><topic>Receptors, Ghrelin - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Wen-jing</creatorcontrib><creatorcontrib>Yuan, Xiao-chen</creatorcontrib><creatorcontrib>Yuan, Yong-chao</creatorcontrib><creatorcontrib>Xie, Shou-qi</creatorcontrib><creatorcontrib>Gong, Yuan</creatorcontrib><creatorcontrib>Su, Hang</creatorcontrib><creatorcontrib>Qiao, Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Wen-jing</au><au>Yuan, Xiao-chen</au><au>Yuan, Yong-chao</au><au>Xie, Shou-qi</au><au>Gong, Yuan</au><au>Su, Hang</au><au>Qiao, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus</atitle><jtitle>Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</jtitle><addtitle>Comp Biochem Physiol A Mol Integr Physiol</addtitle><date>2015-01</date><risdate>2015</risdate><volume>179</volume><spage>54</spage><epage>61</epage><pages>54-61</pages><issn>1095-6433</issn><eissn>1531-4332</eissn><abstract>The growth hormone secretagogue-receptor (GHS-R) is an endogenous receptor for the gut hormone ghrelin. Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a peptide of 360 amino acid residues. Comparison analysis revealed that the amino acid sequences of GHS-R1a were highly conserved in vertebrates and shared 97% amino acid identity with zebrafish (Danio rerio), 96% with jian carp (Cyprinus carpio var. Jian) and 93% with goldfish (Carassius auratus). The GHS-R1a showed the highest level of mRNA expression in the pituitary, followed by the brain and liver, and the lowest expression was observed in the hindgut. Intraperitoneally injected with grass carp ghrelin (50, 100 and 150ng/g body weight (BW)), grass carp showed greater mRNA expression of GHS-R1a in the pituitary compared with saline injected at 0.5h postinjection. It was observed that food deprivation could promote the expression of ghrelin and GHS-R1a in the pituitary, demonstrating that nutritional status can influence the expression of both ghrelin and GHS-R1a in the pituitary. After a 2- or 4-week fast, plasma growth hormone (GH) increased, was positively correlated with ghrelin and GHS-R1a mRNA expression levels in the pituitary. These results suggested that the involvement of ghrelin/GHS-R1a systems in mediating the effects of nutritional status and ghrelin on growth processes in grass carp.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25242546</pmid><doi>10.1016/j.cbpa.2014.09.009</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Carassius auratus Carps - blood Carps - genetics cDNA cloning Cloning, Molecular Ctenopharyngodon idella Cyprinus carpio Danio rerio Energy homeostasis Fasting Food Deprivation Gene Expression Profiling Gene Expression Regulation Genome Ghrelin Ghrelin - administration & dosage Ghrelin - genetics Ghrelin - metabolism GHS-R Grass carp Growth Hormone - blood Molecular Sequence Data Organ Specificity - genetics Phylogeny Pituitary Gland - metabolism Real-Time Polymerase Chain Reaction Receptors, Ghrelin - chemistry Receptors, Ghrelin - genetics Receptors, Ghrelin - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, DNA |
| title | Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus |
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