Quantification of recombinant human erythropoietin by amino acid analysis using isotope dilution liquid chromatography–tandem mass spectrometry

Herein, we describe an accurate method for protein quantification based on conventional acid hydrolysis and an isotope dilution-ultra performance liquid chromatography–tandem mass spectrometry method. The analyte protein, recombinant human erythropoietin (rhEPO), was effectively hydrolyzed by incuba...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2014-07, Vol.406 (18), p.4401-4409
Hauptverfasser:	Yim, Jung-Hyuk, Yoon, Ina, Yang, Hyo-Jin, Kim, Sook-Kyung, Park, Sang-Ryoul, Lee, Yong-Moon, Jeong, Ji-Seon
Format:	Artikel
Sprache:	eng
Schlagworte:	Accuracy Amino acids Amino Acids - analysis Ammonium Analytical Chemistry Biochemistry Biological products Bisphenol A Calibration Capillary electrophoresis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Chromatography, Liquid - methods Erythropoietin Erythropoietin - analysis Food Science Glycoproteins Humans Hydrochloric acid Hydrolysis Laboratory Medicine Liquid chromatography Liquids Mass spectrometry Mathematical analysis Measurement Methods Monitoring/Environmental Analysis Pharmaceutical industry Proteins Quality control Radioisotope Dilution Technique Recombinant Recombinant Proteins - analysis Reference materials Reproducibility Reproducibility of Results Research Paper Scientific imaging Tandem Mass Spectrometry - methods Ultrafiltration
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