Amperometric homogeneous competitive immunoassay in a perfluorocarbon emulsion oxygen therapeutic (PEOT)
The effect of a perfluorocarbon emulsion oxygen therapeutic (PEOT) on the detection of the drugs theophylline and phenytoin was explored using a commercial enzyme multiplied immunoassay technique (EMIT®). The EMIT technique is based on the enzymatic production of NADH, which is typically detected in...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2013-04, Vol.405 (11), p.3541-3547 |
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| creator | Barlag, Rebecca E. Halsall, H. Brian Heineman, William R. |
| description | The effect of a perfluorocarbon emulsion oxygen therapeutic (PEOT) on the detection of the drugs theophylline and phenytoin was explored using a commercial enzyme multiplied immunoassay technique (EMIT®). The EMIT technique is based on the enzymatic production of NADH, which is typically detected in serum samples spectrophotometrically. Here, amperometry using the rotating disk electrode on a single drop of solution is demonstrated to detect theophylline and phenytoin in the presence of PEOT. In the study, 2,6-dichloroindophenol (DCIP) added to the immunoassay mixture is reduced by the NADH to DCIPH
2
. Oxidation of DCIPH
2
is monitored electrochemically at +200 mV using a glassy carbon rotating disk electrode. Slopes of amperograms are proportional to the concentration of drug in the immunoassay sample. This technique yields excellent quantitative data in the therapeutic range for both drugs in 2–20 % PEOT. |
| doi_str_mv | 10.1007/s00216-012-6488-3 |
| format | Article |
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2
. Oxidation of DCIPH
2
is monitored electrochemically at +200 mV using a glassy carbon rotating disk electrode. Slopes of amperograms are proportional to the concentration of drug in the immunoassay sample. This technique yields excellent quantitative data in the therapeutic range for both drugs in 2–20 % PEOT.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-012-6488-3</identifier><identifier>PMID: 23104312</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Anticonvulsants - blood ; Binding sites ; Biochemistry ; Blood substitutes ; Blood Substitutes - analysis ; Bronchodilator Agents - blood ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Conductometric analysis ; Drugs ; Electrical measurements ; Electrochemical Techniques - methods ; Electrodes ; Emittance ; Emulsions ; Enzyme Multiplied Immunoassay Technique ; Enzymes ; Fluorocarbons - blood ; Food Science ; Hemoglobin ; Humans ; Identification and classification ; Immunoassay ; Laboratory Medicine ; Methods ; Monitoring/Environmental Analysis ; Original Paper ; Oxidation ; Patients ; Perfluorocarbons ; Pharmaceutical chemistry ; Phenytoin ; Phenytoin - blood ; Rotating disks ; Sensitivity and Specificity ; Theophylline ; Theophylline - blood</subject><ispartof>Analytical and bioanalytical chemistry, 2013-04, Vol.405 (11), p.3541-3547</ispartof><rights>Springer-Verlag Berlin Heidelberg 2012</rights><rights>COPYRIGHT 2013 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-4e3b21463362fc8fdbf1727e9316f623b443c1c9fc9656aa42cf4802024460c63</citedby><cites>FETCH-LOGICAL-c608t-4e3b21463362fc8fdbf1727e9316f623b443c1c9fc9656aa42cf4802024460c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-012-6488-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-012-6488-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23104312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barlag, Rebecca E.</creatorcontrib><creatorcontrib>Halsall, H. Brian</creatorcontrib><creatorcontrib>Heineman, William R.</creatorcontrib><title>Amperometric homogeneous competitive immunoassay in a perfluorocarbon emulsion oxygen therapeutic (PEOT)</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The effect of a perfluorocarbon emulsion oxygen therapeutic (PEOT) on the detection of the drugs theophylline and phenytoin was explored using a commercial enzyme multiplied immunoassay technique (EMIT®). The EMIT technique is based on the enzymatic production of NADH, which is typically detected in serum samples spectrophotometrically. Here, amperometry using the rotating disk electrode on a single drop of solution is demonstrated to detect theophylline and phenytoin in the presence of PEOT. In the study, 2,6-dichloroindophenol (DCIP) added to the immunoassay mixture is reduced by the NADH to DCIPH
2
. Oxidation of DCIPH
2
is monitored electrochemically at +200 mV using a glassy carbon rotating disk electrode. Slopes of amperograms are proportional to the concentration of drug in the immunoassay sample. This technique yields excellent quantitative data in the therapeutic range for both drugs in 2–20 % PEOT.</description><subject>Analytical Chemistry</subject><subject>Anticonvulsants - blood</subject><subject>Binding sites</subject><subject>Biochemistry</subject><subject>Blood substitutes</subject><subject>Blood Substitutes - analysis</subject><subject>Bronchodilator Agents - blood</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Conductometric analysis</subject><subject>Drugs</subject><subject>Electrical measurements</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Emittance</subject><subject>Emulsions</subject><subject>Enzyme Multiplied Immunoassay Technique</subject><subject>Enzymes</subject><subject>Fluorocarbons - blood</subject><subject>Food Science</subject><subject>Hemoglobin</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Immunoassay</subject><subject>Laboratory Medicine</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Original Paper</subject><subject>Oxidation</subject><subject>Patients</subject><subject>Perfluorocarbons</subject><subject>Pharmaceutical chemistry</subject><subject>Phenytoin</subject><subject>Phenytoin - blood</subject><subject>Rotating disks</subject><subject>Sensitivity and Specificity</subject><subject>Theophylline</subject><subject>Theophylline - blood</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl9vFCEUxSdGY-vqB_DFTOJLfZh6LzB32MdNU_8kTepDfSYMC7s0w7DCjHG_vaxbm2o0Cg_cwO8cuORU1UuEcwTo3mYAhtQAsoaElA1_VJ0ioWwYtfD4vhbspHqW8y0AthLpaXXCOILgyE6r7SrsbIrBTsmbehtD3NjRxjnXJpaTyU_-q619CPMYdc56X_ux1nXRuGGOKRqd-jjWNsxD9qWI3_bFoJ62Numdnadievbp8vrmzfPqidNDti_u1kX1-d3lzcWH5ur6_ceL1VVjCOTUCMt7hoI4J-aMdOveYcc6u-RIjhjvheAGzdKZJbWktWDGCQkMmBAEhviiOjv67lL8Mts8qeCzscOgf7SlkKh8HZJs_40Kvuy4JPgPlDMsT4YWCvr6N_Q2zmksPZe7O2CMpHhAbfRglR9dnJI2B1O14lwCl7yMRXX-B6rMtQ3exNE6X_Z_EeBRYFLMOVmndskHnfYKQR0yo46ZUSUz6pAZddC8unvw3Ae7vlf8DEkB2BHI5Wjc2PSgo7-6fgcbNck3</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Barlag, Rebecca E.</creator><creator>Halsall, H. 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Brian</au><au>Heineman, William R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric homogeneous competitive immunoassay in a perfluorocarbon emulsion oxygen therapeutic (PEOT)</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>405</volume><issue>11</issue><spage>3541</spage><epage>3547</epage><pages>3541-3547</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The effect of a perfluorocarbon emulsion oxygen therapeutic (PEOT) on the detection of the drugs theophylline and phenytoin was explored using a commercial enzyme multiplied immunoassay technique (EMIT®). The EMIT technique is based on the enzymatic production of NADH, which is typically detected in serum samples spectrophotometrically. Here, amperometry using the rotating disk electrode on a single drop of solution is demonstrated to detect theophylline and phenytoin in the presence of PEOT. In the study, 2,6-dichloroindophenol (DCIP) added to the immunoassay mixture is reduced by the NADH to DCIPH
2
. Oxidation of DCIPH
2
is monitored electrochemically at +200 mV using a glassy carbon rotating disk electrode. Slopes of amperograms are proportional to the concentration of drug in the immunoassay sample. This technique yields excellent quantitative data in the therapeutic range for both drugs in 2–20 % PEOT.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23104312</pmid><doi>10.1007/s00216-012-6488-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2013-04, Vol.405 (11), p.3541-3547 |
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| subjects | Analytical Chemistry Anticonvulsants - blood Binding sites Biochemistry Blood substitutes Blood Substitutes - analysis Bronchodilator Agents - blood Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Conductometric analysis Drugs Electrical measurements Electrochemical Techniques - methods Electrodes Emittance Emulsions Enzyme Multiplied Immunoassay Technique Enzymes Fluorocarbons - blood Food Science Hemoglobin Humans Identification and classification Immunoassay Laboratory Medicine Methods Monitoring/Environmental Analysis Original Paper Oxidation Patients Perfluorocarbons Pharmaceutical chemistry Phenytoin Phenytoin - blood Rotating disks Sensitivity and Specificity Theophylline Theophylline - blood |
| title | Amperometric homogeneous competitive immunoassay in a perfluorocarbon emulsion oxygen therapeutic (PEOT) |
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