Genetic analysis of lipolytic activities in Thermus thermophilus HB27

•Putative lipase genes of Thermus thermophilus HB27 were mutated systematically.•A new deletion strategy for genome modification in T. thermophilus was established.•Contribution of each predicted lipase on total activity in T. thermophilus is shown.•An esterase-less T. thermophilus multi-deletion mu...

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Veröffentlicht in:	Journal of biotechnology 2014-12, Vol.191, p.150-157
Hauptverfasser:	Leis, Benedikt, Angelov, Angel, Li, Haijuan, Liebl, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbon Deletion Enzymes Esterases Esterases - chemistry Esterases - genetics Esters Genes Genetic analysis Humans Hydrolysis Lipase - chemistry Lipase - genetics Lipolysis - genetics Lipolytic activity Mutation Novel clean deletion system Strain T. thermophilus HB27 Thermostable esterase Thermus thermophilus Thermus thermophilus - genetics Thermus thermophilus - metabolism Triglycerides - chemistry
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