Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes
Lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of Listeria monocytogenes in food matrices. The target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2013-04, Vol.405 (10), p.3313-3319 |
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| creator | Cho, Il-Hoon Irudayaraj, Joseph |
| description | Lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of
Listeria monocytogenes
in food matrices. The target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen–antibody reaction without any additional steps. The color signals produced by an enzyme–substrate reaction at a specific site on the immunostrip were found to be directly proportional to the concentration of
L. monocytogenes
in the sample. The detection concept was demonstrated by performing an enzyme immunoassay on a microtiter well prior to applying it to the lateral-flow assay. Results of the chromatographic analysis yield a limit of detection of 95 and 97 ± 19.5 CFU/mL in buffer solution and 2 % milk sample, respectively. In addition to the high sensitivity, it was also possible to shorten the separation and detection time to within 2 h. The system also showed no cross-reactivity with other bacteria (e.g.,
Escherichia coli
O157:H7,
Salmonella typhimurium
, and
Salmonella enteritidis
). The analytical procedure developed will enable us to not only utilize the assay in the field where fast screening for pathogenic agents is required but also as a preventive measure to contain disease outbreak. |
| doi_str_mv | 10.1007/s00216-013-6742-3 |
| format | Article |
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Listeria monocytogenes
in food matrices. The target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen–antibody reaction without any additional steps. The color signals produced by an enzyme–substrate reaction at a specific site on the immunostrip were found to be directly proportional to the concentration of
L. monocytogenes
in the sample. The detection concept was demonstrated by performing an enzyme immunoassay on a microtiter well prior to applying it to the lateral-flow assay. Results of the chromatographic analysis yield a limit of detection of 95 and 97 ± 19.5 CFU/mL in buffer solution and 2 % milk sample, respectively. In addition to the high sensitivity, it was also possible to shorten the separation and detection time to within 2 h. The system also showed no cross-reactivity with other bacteria (e.g.,
Escherichia coli
O157:H7,
Salmonella typhimurium
, and
Salmonella enteritidis
). The analytical procedure developed will enable us to not only utilize the assay in the field where fast screening for pathogenic agents is required but also as a preventive measure to contain disease outbreak.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-013-6742-3</identifier><identifier>PMID: 23354582</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Animals ; Assaying ; Bacteria ; Biochemistry ; Cattle ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography - methods ; Color ; E coli ; Enzymes ; Escherichia coli ; Escherichia coli O157 - isolation & purification ; Food ; Food Contamination - analysis ; Food Science ; Immunoassay ; Immunochemistry ; Immunoenzyme Techniques - methods ; Immunology ; Laboratory Medicine ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - isolation & purification ; Mathematical analysis ; Methods ; Microbiological chemistry ; Microorganisms ; Milk - chemistry ; Milk - microbiology ; Monitoring/Environmental Analysis ; Original Paper ; Physiological aspects ; Salmonella ; Salmonella enteritidis ; Salmonella enteritidis - isolation & purification ; Salmonella typhimurium ; Salmonella typhimurium - isolation & purification</subject><ispartof>Analytical and bioanalytical chemistry, 2013-04, Vol.405 (10), p.3313-3319</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>COPYRIGHT 2013 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-8efb5c4b6035ade0900f6c2bbe21d249b6420b81ee0e32017614f2510349fa2f3</citedby><cites>FETCH-LOGICAL-c542t-8efb5c4b6035ade0900f6c2bbe21d249b6420b81ee0e32017614f2510349fa2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-013-6742-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-013-6742-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23354582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Il-Hoon</creatorcontrib><creatorcontrib>Irudayaraj, Joseph</creatorcontrib><title>Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of
Listeria monocytogenes
in food matrices. The target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen–antibody reaction without any additional steps. The color signals produced by an enzyme–substrate reaction at a specific site on the immunostrip were found to be directly proportional to the concentration of
L. monocytogenes
in the sample. The detection concept was demonstrated by performing an enzyme immunoassay on a microtiter well prior to applying it to the lateral-flow assay. Results of the chromatographic analysis yield a limit of detection of 95 and 97 ± 19.5 CFU/mL in buffer solution and 2 % milk sample, respectively. In addition to the high sensitivity, it was also possible to shorten the separation and detection time to within 2 h. The system also showed no cross-reactivity with other bacteria (e.g.,
Escherichia coli
O157:H7,
Salmonella typhimurium
, and
Salmonella enteritidis
). The analytical procedure developed will enable us to not only utilize the assay in the field where fast screening for pathogenic agents is required but also as a preventive measure to contain disease outbreak.</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Cattle</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography - methods</subject><subject>Color</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Food</subject><subject>Food Contamination - analysis</subject><subject>Food Science</subject><subject>Immunoassay</subject><subject>Immunochemistry</subject><subject>Immunoenzyme Techniques - methods</subject><subject>Immunology</subject><subject>Laboratory Medicine</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>Microbiological chemistry</subject><subject>Microorganisms</subject><subject>Milk - chemistry</subject><subject>Milk - microbiology</subject><subject>Monitoring/Environmental Analysis</subject><subject>Original Paper</subject><subject>Physiological aspects</subject><subject>Salmonella</subject><subject>Salmonella enteritidis</subject><subject>Salmonella enteritidis - isolation & purification</subject><subject>Salmonella typhimurium</subject><subject>Salmonella typhimurium - isolation & purification</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1v1DAQhiMEoqXwA7igSFy4pMz4K86xqoAircSFni0nGa9cJfZiJ0LLr6-XbcuHQJUPtsbPO54Zv1X1GuEcAdr3GYChagB5o1rBGv6kOkWFumFKwtOHs2An1YucbwBQalTPqxPGuRRSs9PqemMXSnZq3BS_1xR-7Geq_TyvIQ4xDBSWZBcfQ-1iqpPd-bEeaaHhZyy6euNz0Xtbz7Eo9kvcUqD8snrm7JTp1d1-Vl1__PD18qrZfPn0-fJi0wxSsKXR5Ho5iF4Bl3Yk6ACcGljfE8ORia4vpUOvkQiIM8BWoXBMInDROcscP6veHfPuUvy2Ul7M7PNA02QDxTUbVApAIRfwOCo4aNm1Eh9HObZaqE4f0Ld_oTdxTaH0XN5ugZXhI_yitnYi44OLZarDIam54FwD1xzaQp3_gyprpNmXzyDnS_wPAR4FQ4o5J3Jml_xs094gmINBzNEgphjEHAxieNG8uSt47WcaHxT3jigAOwK5XIUtpd86-m_WW4XDwr0</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Cho, Il-Hoon</creator><creator>Irudayaraj, Joseph</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7QH</scope><scope>7QL</scope><scope>7UA</scope></search><sort><creationdate>20130401</creationdate><title>Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes</title><author>Cho, Il-Hoon ; Irudayaraj, Joseph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-8efb5c4b6035ade0900f6c2bbe21d249b6420b81ee0e32017614f2510349fa2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Cattle</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography - 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Academic</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Water Resources Abstracts</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Il-Hoon</au><au>Irudayaraj, Joseph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>405</volume><issue>10</issue><spage>3313</spage><epage>3319</epage><pages>3313-3319</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of
Listeria monocytogenes
in food matrices. The target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen–antibody reaction without any additional steps. The color signals produced by an enzyme–substrate reaction at a specific site on the immunostrip were found to be directly proportional to the concentration of
L. monocytogenes
in the sample. The detection concept was demonstrated by performing an enzyme immunoassay on a microtiter well prior to applying it to the lateral-flow assay. Results of the chromatographic analysis yield a limit of detection of 95 and 97 ± 19.5 CFU/mL in buffer solution and 2 % milk sample, respectively. In addition to the high sensitivity, it was also possible to shorten the separation and detection time to within 2 h. The system also showed no cross-reactivity with other bacteria (e.g.,
Escherichia coli
O157:H7,
Salmonella typhimurium
, and
Salmonella enteritidis
). The analytical procedure developed will enable us to not only utilize the assay in the field where fast screening for pathogenic agents is required but also as a preventive measure to contain disease outbreak.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23354582</pmid><doi>10.1007/s00216-013-6742-3</doi><tpages>7</tpages></addata></record> |
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| issn | 1618-2642 1618-2650 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Animals Assaying Bacteria Biochemistry Cattle Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Chromatography - methods Color E coli Enzymes Escherichia coli Escherichia coli O157 - isolation & purification Food Food Contamination - analysis Food Science Immunoassay Immunochemistry Immunoenzyme Techniques - methods Immunology Laboratory Medicine Listeria Listeria monocytogenes Listeria monocytogenes - isolation & purification Mathematical analysis Methods Microbiological chemistry Microorganisms Milk - chemistry Milk - microbiology Monitoring/Environmental Analysis Original Paper Physiological aspects Salmonella Salmonella enteritidis Salmonella enteritidis - isolation & purification Salmonella typhimurium Salmonella typhimurium - isolation & purification |
| title | Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes |
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