Characterization of cefalexin degradation capabilities of two Pseudomonas strains isolated from activated sludge
•Isolation of two cefalexin-degrading bacteria from activated sludge.•Over 90% degradation of cefalexin within a day by strain CE22.•Bacterial degradation on cefalexin affected by presence of other pharmaceuticals.•2-Hydroxy-3-phenyl pyrazine was produced after bacterial degradation of cefalexin.•St...
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Veröffentlicht in: | Journal of hazardous materials 2015-01, Vol.282, p.158-164 |
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creator | Lin, Bokun Lyu, Jinling Lyu, Xian-jin Yu, Han-qing Hu, Zhong Lam, James C.W. Lam, Paul K.S. |
description | •Isolation of two cefalexin-degrading bacteria from activated sludge.•Over 90% degradation of cefalexin within a day by strain CE22.•Bacterial degradation on cefalexin affected by presence of other pharmaceuticals.•2-Hydroxy-3-phenyl pyrazine was produced after bacterial degradation of cefalexin.•Strains CE21 and CE22 can degrade multiple pharmaceuticals.
Pharmaceuticals have recently been regarded as contaminants of emerging concern. To date, there is limited knowledge about antibiotic-degrading microorganisms in conventional activated sludge treatment systems and their characteristics toward antibiotic degradation especially in the presence of a pharmaceutical mixture. As such, antibiotic-degrading microorganisms were investigated and isolated from the activated sludge, and their degradation capabilities were evaluated. Two strains of cefalexin-degrading bacteria CE21 and CE22 were isolated and identified as Pseudomonas sp. in the collected activated sludge. Strain CE22 was able to degrade over 90% of cefalexin, while CE21 was able to remove 46.7% of cefalexin after incubation for 24h. The removal efficiency of cefalexin by CE22, different from that of CE21, was not significantly affected by an increase in cefalexin concentration, even up to 10ppm, however the presence of 1ppm of other pharmaceuticals had a significant effect on the degradation of cefalexin by CE22, but no significant effect on CE21. The degradation product of cefalexin by the two strains was identified to be 2-hydroxy-3-phenyl pyrazine. Our results also indicated that CE21 and CE22 were able to degrade caffeine, salicylic acid and chloramphenicol. Moreover, CE21 was found to be capable of eliminating sulfamethoxazole and naproxen. |
doi_str_mv | 10.1016/j.jhazmat.2014.06.080 |
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Pharmaceuticals have recently been regarded as contaminants of emerging concern. To date, there is limited knowledge about antibiotic-degrading microorganisms in conventional activated sludge treatment systems and their characteristics toward antibiotic degradation especially in the presence of a pharmaceutical mixture. As such, antibiotic-degrading microorganisms were investigated and isolated from the activated sludge, and their degradation capabilities were evaluated. Two strains of cefalexin-degrading bacteria CE21 and CE22 were isolated and identified as Pseudomonas sp. in the collected activated sludge. Strain CE22 was able to degrade over 90% of cefalexin, while CE21 was able to remove 46.7% of cefalexin after incubation for 24h. The removal efficiency of cefalexin by CE22, different from that of CE21, was not significantly affected by an increase in cefalexin concentration, even up to 10ppm, however the presence of 1ppm of other pharmaceuticals had a significant effect on the degradation of cefalexin by CE22, but no significant effect on CE21. The degradation product of cefalexin by the two strains was identified to be 2-hydroxy-3-phenyl pyrazine. Our results also indicated that CE21 and CE22 were able to degrade caffeine, salicylic acid and chloramphenicol. Moreover, CE21 was found to be capable of eliminating sulfamethoxazole and naproxen.</description><identifier>ISSN: 0304-3894</identifier><identifier>EISSN: 1873-3336</identifier><identifier>DOI: 10.1016/j.jhazmat.2014.06.080</identifier><identifier>PMID: 25070321</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Activated sludge ; Anti-Bacterial Agents - metabolism ; Antibiotics ; Bacteria ; Biodegradation ; Caffeine - metabolism ; Cefalexin ; Cephalexin - metabolism ; Chloramphenicol - metabolism ; Degradation ; Microorganisms ; Naproxen - metabolism ; Pharmaceuticals ; Pseudomonas ; Pseudomonas - genetics ; Pseudomonas - isolation & purification ; Pseudomonas - metabolism ; Pyrazines - metabolism ; RNA, Bacterial - analysis ; RNA, Ribosomal, 16S - analysis ; Salicylic Acid - metabolism ; Sequence Analysis, RNA ; Sewage - microbiology ; Strain ; Sulfamethoxazole - metabolism ; Water Pollutants, Chemical - metabolism</subject><ispartof>Journal of hazardous materials, 2015-01, Vol.282, p.158-164</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-99f127c232cd069ca541b9e437e232a739312ef399b8d302cc564bc7476c04873</citedby><cites>FETCH-LOGICAL-c538t-99f127c232cd069ca541b9e437e232a739312ef399b8d302cc564bc7476c04873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jhazmat.2014.06.080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25070321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Bokun</creatorcontrib><creatorcontrib>Lyu, Jinling</creatorcontrib><creatorcontrib>Lyu, Xian-jin</creatorcontrib><creatorcontrib>Yu, Han-qing</creatorcontrib><creatorcontrib>Hu, Zhong</creatorcontrib><creatorcontrib>Lam, James C.W.</creatorcontrib><creatorcontrib>Lam, Paul K.S.</creatorcontrib><title>Characterization of cefalexin degradation capabilities of two Pseudomonas strains isolated from activated sludge</title><title>Journal of hazardous materials</title><addtitle>J Hazard Mater</addtitle><description>•Isolation of two cefalexin-degrading bacteria from activated sludge.•Over 90% degradation of cefalexin within a day by strain CE22.•Bacterial degradation on cefalexin affected by presence of other pharmaceuticals.•2-Hydroxy-3-phenyl pyrazine was produced after bacterial degradation of cefalexin.•Strains CE21 and CE22 can degrade multiple pharmaceuticals.
Pharmaceuticals have recently been regarded as contaminants of emerging concern. To date, there is limited knowledge about antibiotic-degrading microorganisms in conventional activated sludge treatment systems and their characteristics toward antibiotic degradation especially in the presence of a pharmaceutical mixture. As such, antibiotic-degrading microorganisms were investigated and isolated from the activated sludge, and their degradation capabilities were evaluated. Two strains of cefalexin-degrading bacteria CE21 and CE22 were isolated and identified as Pseudomonas sp. in the collected activated sludge. Strain CE22 was able to degrade over 90% of cefalexin, while CE21 was able to remove 46.7% of cefalexin after incubation for 24h. The removal efficiency of cefalexin by CE22, different from that of CE21, was not significantly affected by an increase in cefalexin concentration, even up to 10ppm, however the presence of 1ppm of other pharmaceuticals had a significant effect on the degradation of cefalexin by CE22, but no significant effect on CE21. The degradation product of cefalexin by the two strains was identified to be 2-hydroxy-3-phenyl pyrazine. Our results also indicated that CE21 and CE22 were able to degrade caffeine, salicylic acid and chloramphenicol. Moreover, CE21 was found to be capable of eliminating sulfamethoxazole and naproxen.</description><subject>Activated sludge</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Caffeine - metabolism</subject><subject>Cefalexin</subject><subject>Cephalexin - metabolism</subject><subject>Chloramphenicol - metabolism</subject><subject>Degradation</subject><subject>Microorganisms</subject><subject>Naproxen - metabolism</subject><subject>Pharmaceuticals</subject><subject>Pseudomonas</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - isolation & purification</subject><subject>Pseudomonas - metabolism</subject><subject>Pyrazines - metabolism</subject><subject>RNA, Bacterial - analysis</subject><subject>RNA, Ribosomal, 16S - analysis</subject><subject>Salicylic Acid - metabolism</subject><subject>Sequence Analysis, RNA</subject><subject>Sewage - microbiology</subject><subject>Strain</subject><subject>Sulfamethoxazole - metabolism</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>0304-3894</issn><issn>1873-3336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi1ERZfCTwDlyCVh_BE7OSG0ooBUiR7gbDn2pPUqiRfbaWl_PV524UpPlu1n3hnNQ8gbCg0FKt_vmt2teZxNbhhQ0YBsoINnZEM7xWvOuXxONsBB1LzrxTl5mdIOAKhqxQtyzlpQwBndkP321kRjM0b_aLIPSxXGyuJoJvzll8rhTTTu-GHN3gx-8tljOlD5PlTXCVcX5rCYVKUcjV9S5VOYTEZXjTHMVcn2d3-uaVrdDb4iZyU84evTeUF-XH76vv1SX337_HX78aq2Le9y3fcjZcoyzqwD2VvTCjr0KLjC8mYU7zllOPK-HzrHgVnbSjFYJZS0IMoOLsi7Y-4-hp8rpqxnnyxOk1kwrElTKQFKqIQnoEIB9KDkE1AuWBmCHQZoj6iNIaWIo95HP5v4oCnog0K90yeF-qBQg9RFYal7e2qxDjO6f1V_nRXgwxHAsr47j1En63Gx6HxEm7UL_j8tfgNRmrAa</recordid><startdate>20150123</startdate><enddate>20150123</enddate><creator>Lin, Bokun</creator><creator>Lyu, Jinling</creator><creator>Lyu, Xian-jin</creator><creator>Yu, Han-qing</creator><creator>Hu, Zhong</creator><creator>Lam, James C.W.</creator><creator>Lam, Paul K.S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7QL</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>7QQ</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>KR7</scope></search><sort><creationdate>20150123</creationdate><title>Characterization of cefalexin degradation capabilities of two Pseudomonas strains isolated from activated sludge</title><author>Lin, Bokun ; Lyu, Jinling ; Lyu, Xian-jin ; Yu, Han-qing ; Hu, Zhong ; Lam, James C.W. ; Lam, Paul K.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-99f127c232cd069ca541b9e437e232a739312ef399b8d302cc564bc7476c04873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Activated sludge</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Caffeine - metabolism</topic><topic>Cefalexin</topic><topic>Cephalexin - metabolism</topic><topic>Chloramphenicol - metabolism</topic><topic>Degradation</topic><topic>Microorganisms</topic><topic>Naproxen - metabolism</topic><topic>Pharmaceuticals</topic><topic>Pseudomonas</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - isolation & purification</topic><topic>Pseudomonas - metabolism</topic><topic>Pyrazines - metabolism</topic><topic>RNA, Bacterial - analysis</topic><topic>RNA, Ribosomal, 16S - analysis</topic><topic>Salicylic Acid - metabolism</topic><topic>Sequence Analysis, RNA</topic><topic>Sewage - microbiology</topic><topic>Strain</topic><topic>Sulfamethoxazole - metabolism</topic><topic>Water Pollutants, Chemical - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Bokun</creatorcontrib><creatorcontrib>Lyu, Jinling</creatorcontrib><creatorcontrib>Lyu, Xian-jin</creatorcontrib><creatorcontrib>Yu, Han-qing</creatorcontrib><creatorcontrib>Hu, Zhong</creatorcontrib><creatorcontrib>Lam, James C.W.</creatorcontrib><creatorcontrib>Lam, Paul K.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Journal of hazardous materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Bokun</au><au>Lyu, Jinling</au><au>Lyu, Xian-jin</au><au>Yu, Han-qing</au><au>Hu, Zhong</au><au>Lam, James C.W.</au><au>Lam, Paul K.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of cefalexin degradation capabilities of two Pseudomonas strains isolated from activated sludge</atitle><jtitle>Journal of hazardous materials</jtitle><addtitle>J Hazard Mater</addtitle><date>2015-01-23</date><risdate>2015</risdate><volume>282</volume><spage>158</spage><epage>164</epage><pages>158-164</pages><issn>0304-3894</issn><eissn>1873-3336</eissn><abstract>•Isolation of two cefalexin-degrading bacteria from activated sludge.•Over 90% degradation of cefalexin within a day by strain CE22.•Bacterial degradation on cefalexin affected by presence of other pharmaceuticals.•2-Hydroxy-3-phenyl pyrazine was produced after bacterial degradation of cefalexin.•Strains CE21 and CE22 can degrade multiple pharmaceuticals.
Pharmaceuticals have recently been regarded as contaminants of emerging concern. To date, there is limited knowledge about antibiotic-degrading microorganisms in conventional activated sludge treatment systems and their characteristics toward antibiotic degradation especially in the presence of a pharmaceutical mixture. As such, antibiotic-degrading microorganisms were investigated and isolated from the activated sludge, and their degradation capabilities were evaluated. Two strains of cefalexin-degrading bacteria CE21 and CE22 were isolated and identified as Pseudomonas sp. in the collected activated sludge. Strain CE22 was able to degrade over 90% of cefalexin, while CE21 was able to remove 46.7% of cefalexin after incubation for 24h. The removal efficiency of cefalexin by CE22, different from that of CE21, was not significantly affected by an increase in cefalexin concentration, even up to 10ppm, however the presence of 1ppm of other pharmaceuticals had a significant effect on the degradation of cefalexin by CE22, but no significant effect on CE21. The degradation product of cefalexin by the two strains was identified to be 2-hydroxy-3-phenyl pyrazine. Our results also indicated that CE21 and CE22 were able to degrade caffeine, salicylic acid and chloramphenicol. Moreover, CE21 was found to be capable of eliminating sulfamethoxazole and naproxen.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25070321</pmid><doi>10.1016/j.jhazmat.2014.06.080</doi><tpages>7</tpages></addata></record> |
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subjects | Activated sludge Anti-Bacterial Agents - metabolism Antibiotics Bacteria Biodegradation Caffeine - metabolism Cefalexin Cephalexin - metabolism Chloramphenicol - metabolism Degradation Microorganisms Naproxen - metabolism Pharmaceuticals Pseudomonas Pseudomonas - genetics Pseudomonas - isolation & purification Pseudomonas - metabolism Pyrazines - metabolism RNA, Bacterial - analysis RNA, Ribosomal, 16S - analysis Salicylic Acid - metabolism Sequence Analysis, RNA Sewage - microbiology Strain Sulfamethoxazole - metabolism Water Pollutants, Chemical - metabolism |
title | Characterization of cefalexin degradation capabilities of two Pseudomonas strains isolated from activated sludge |
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