A signal “on” photoelectrochemical biosensor for assay of protein kinase activity and its inhibitor based on graphite-like carbon nitride, Phos-tag and alkaline phosphatase

A highly sensitive and selective photoelectrochemical (PEC) biosensor is fabricated for the detection of protein kinase activity based on visible-light active graphite-like carbon nitride (g-C3N4) and the specific recognition utility of Phos-tag for protein kinase A (PKA)-induced phosphopeptides. Fo...

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Veröffentlicht in:	Biosensors & bioelectronics 2015-02, Vol.64, p.462-468
Hauptverfasser:	Yin, Huanshun, Sun, Bing, Dong, Linfeng, Li, Bingchen, Zhou, Yunlei, Ai, Shiyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline phosphatase Alkaline Phosphatase - chemistry Biosensing Techniques - instrumentation Biosensors Biotin Carbon nitride Cell lysate Conductometry - instrumentation Electrodes Enzyme Activation Equipment Design Equipment Failure Analysis Graphite - chemistry Graphite-like carbon nitride Inhibitor Inhibitors Kinases Nitriles - chemistry Peptides Phos-tag Photoelectrochemical biosensor Photometry - instrumentation Protein kinase activity Protein Kinase Inhibitors - analysis Protein Kinase Inhibitors - chemistry Protein Kinases - analysis Protein Kinases - chemistry Proteins Pyridines - chemistry
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