Determination of sulfite with emphasis on biosensing methods: a review

Sulfite is used as a preservative in a variety of food and pharmaceutical industries to inhibit enzymatic and nonenzymatic browning and in brewing industries as an antibacterial and antioxidizing agent. Convenient and reproducible analytical methods employing sulfite oxidase are an attractive altern...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2013-04, Vol.405 (10), p.3049-3062
Hauptverfasser: Pundir, Chandra Shekhar, Rawal, Rachna
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Rawal, Rachna
description Sulfite is used as a preservative in a variety of food and pharmaceutical industries to inhibit enzymatic and nonenzymatic browning and in brewing industries as an antibacterial and antioxidizing agent. Convenient and reproducible analytical methods employing sulfite oxidase are an attractive alternative to conventional detection methods. Sulfite biosensors are based on measurement of either O 2 or electrons generated from splitting of H 2 O 2 or heat released during oxidation of sulfite by immobilized sulfite oxidase. Sulfite biosensors can be grouped into 12 classes. They work optimally within 2 to 900 s, between pH 6.5 and 9.0, 25 and 40 °C, and in the range from 0 to 50,000 μM, with detection limit between 0.2 and 200 μM. Sulfite biosensors measure sulfite in food, beverages, and water and can be reused 100–300 times over a period of 1–240 days. The review presents the principles, merits, and demerits of various analytical methods for determination of sulfite, with special emphasis on sulfite biosensors.
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source MEDLINE; SpringerNature Journals
subjects Analytical Chemistry
Analytical methods
Antiinfectives and antibacterials
Automation
Beverages
Biochemistry
Biosensing Techniques - methods
Biosensors
Browning
Capillary electrophoresis
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Composition
Cytochrome
Food
Food Analysis
Food Preservatives - analysis
Food Science
Foods
Fruits
Hydrogen peroxide
Identification and classification
Iodine
Laboratory Medicine
Mathematical analysis
Methods
Monitoring/Environmental Analysis
Oxidase
Oxidation
Pharmaceutical industry
Preservatives
Review
Sodium
Sulfites
Sulfites - analysis
Sulfur
Testing
Water chemistry
title Determination of sulfite with emphasis on biosensing methods: a review
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