Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942
The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of t...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1993-08, Vol.59 (8), p.2404-2410 |
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| creator | SOLTES-RAK, E KUSHNER, D. J WILLIAMS, D. D COLEMAN, J. R |
| description | The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter upstream of the cryIVB sequence. Replacement of the endogenous corresponding Bacillus sequences with the Synechococcus petF1 promoter, ribosome binding site, and initiation codon also resulted in increased expression of the cryIVB gene relative to the expression obtained with the Bacillus promoter alone but decreased expression relative to the expression achieved with the tandem array of the Bacillus and lacZ promoters. Synechococcus cells carrying plasmids in which the expression of the cryIVB gene was regulated by either the lacZ or the petF1 promoter were readily consumed by first-instar Culex restuans larvae and proved to be toxic for these organisms. |
| doi_str_mv | 10.1128/aem.59.8.2404-2410.1993 |
| format | Article |
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J ; WILLIAMS, D. D ; COLEMAN, J. R</creator><creatorcontrib>SOLTES-RAK, E ; KUSHNER, D. J ; WILLIAMS, D. D ; COLEMAN, J. R</creatorcontrib><description>The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter upstream of the cryIVB sequence. Replacement of the endogenous corresponding Bacillus sequences with the Synechococcus petF1 promoter, ribosome binding site, and initiation codon also resulted in increased expression of the cryIVB gene relative to the expression obtained with the Bacillus promoter alone but decreased expression relative to the expression achieved with the tandem array of the Bacillus and lacZ promoters. Synechococcus cells carrying plasmids in which the expression of the cryIVB gene was regulated by either the lacZ or the petF1 promoter were readily consumed by first-instar Culex restuans larvae and proved to be toxic for these organisms.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.59.8.2404-2410.1993</identifier><identifier>PMID: 7690220</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>adn recombinado ; adn recombine ; bacillus thuringiensis ; Bacillus thuringiensis - genetics ; Bacillus thuringiensis israelensis ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Toxins ; Biological and medical sciences ; Biotechnology ; Culex restuans ; Cyanobacteria - genetics ; cyanophyta ; endotoxinas ; endotoxine ; endotoxins ; Endotoxins - genetics ; Escherichia coli - genetics ; expresion genica ; expression des genes ; Freshwater ; Fundamental and applied biological sciences. Psychology ; gene ; Gene Expression ; Genes ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; genetic transformation ; Genetic Vectors ; genetica ; genetics ; genetique ; Hemolysin Proteins ; insecticidas ; insecticide ; insecticides ; Insects ; larvae ; larvas ; larve ; Methods. Procedures. Technologies ; Modification of gene expression level ; Pest Control, Biological ; Plasmids - genetics ; Promoter Regions, Genetic ; recombinant dna ; RNA, Bacterial - genetics ; RNA, Messenger - genetics ; Synechococcus ; toxinas ; toxine ; toxins ; transformacion genetica ; transformation genetique ; Transformation, Genetic</subject><ispartof>Applied and Environmental Microbiology, 1993-08, Vol.59 (8), p.2404-2410</ispartof><rights>1993 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Aug 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-480a0d204354952af508e7be8033cf64d5d255a21062c43ca21e01ae4a14a79b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC182298/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC182298/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4889756$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7690220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOLTES-RAK, E</creatorcontrib><creatorcontrib>KUSHNER, D. J</creatorcontrib><creatorcontrib>WILLIAMS, D. D</creatorcontrib><creatorcontrib>COLEMAN, J. R</creatorcontrib><title>Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter upstream of the cryIVB sequence. Replacement of the endogenous corresponding Bacillus sequences with the Synechococcus petF1 promoter, ribosome binding site, and initiation codon also resulted in increased expression of the cryIVB gene relative to the expression obtained with the Bacillus promoter alone but decreased expression relative to the expression achieved with the tandem array of the Bacillus and lacZ promoters. Synechococcus cells carrying plasmids in which the expression of the cryIVB gene was regulated by either the lacZ or the petF1 promoter were readily consumed by first-instar Culex restuans larvae and proved to be toxic for these organisms.</description><subject>adn recombinado</subject><subject>adn recombine</subject><subject>bacillus thuringiensis</subject><subject>Bacillus thuringiensis - genetics</subject><subject>Bacillus thuringiensis israelensis</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Culex restuans</subject><subject>Cyanobacteria - genetics</subject><subject>cyanophyta</subject><subject>endotoxinas</subject><subject>endotoxine</subject><subject>endotoxins</subject><subject>Endotoxins - genetics</subject><subject>Escherichia coli - genetics</subject><subject>expresion genica</subject><subject>expression des genes</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>genetica</subject><subject>genetics</subject><subject>genetique</subject><subject>Hemolysin Proteins</subject><subject>insecticidas</subject><subject>insecticide</subject><subject>insecticides</subject><subject>Insects</subject><subject>larvae</subject><subject>larvas</subject><subject>larve</subject><subject>Methods. Procedures. Technologies</subject><subject>Modification of gene expression level</subject><subject>Pest Control, Biological</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant dna</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Synechococcus</subject><subject>toxinas</subject><subject>toxine</subject><subject>toxins</subject><subject>transformacion genetica</subject><subject>transformation genetique</subject><subject>Transformation, Genetic</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkVGP1CAUhYnRrOPqT1DRGN9aLxRaeNgHnay6ySaarOsrYSjMsGlLF1p1_r00M5m4JiQQzncu93IQek2gJISKD9r2JZelKCkDVlC23EtZPUIrAlIUvKrqx2gFIGVBM_IUPUvpDgAY1OIMnTW1BEphhbpL56yZcHB4jKEPk424D6133ujJhwHn1Yd0P_spGN_qDpu4v_r5CW_tYLH9M0ab0sL5Ad_sB2t2wQRj5oTTWOI0RZ2F7-s1biSjz9ETp7tkXxz3c3T7-fLH-mtx_e3L1frjdWE4a6aCCdDQUmAVZ5JT7TgI22ysgKoyrmYtbynnmhKoqWGVyScLRFumCdON3FTn6OJQd5w3vW2NHXIfnRqj73Xcq6C9eqgMfqe24ZciglIpsv_90R_D_WzTpHqfjO06PdgwJ0XqOv8kpRl8-x94F-Y45NkUBS5pQ_kCNQfIxJBStO7UCAG1hKlymIpLJdQSplrCVEuY2fny3zlOvmN6WX931HUyunNRD8anE8aEkA2vM_bmgO38dvfbR6t06h8-mplXB8bpoPQ25jK3N7kJBiCAU1b9BYDcvJ8</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>SOLTES-RAK, E</creator><creator>KUSHNER, D. J</creator><creator>WILLIAMS, D. D</creator><creator>COLEMAN, J. R</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>19930801</creationdate><title>Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942</title><author>SOLTES-RAK, E ; KUSHNER, D. J ; WILLIAMS, D. D ; COLEMAN, J. 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Psychology</topic><topic>gene</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>genetica</topic><topic>genetics</topic><topic>genetique</topic><topic>Hemolysin Proteins</topic><topic>insecticidas</topic><topic>insecticide</topic><topic>insecticides</topic><topic>Insects</topic><topic>larvae</topic><topic>larvas</topic><topic>larve</topic><topic>Methods. Procedures. Technologies</topic><topic>Modification of gene expression level</topic><topic>Pest Control, Biological</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant dna</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Synechococcus</topic><topic>toxinas</topic><topic>toxine</topic><topic>toxins</topic><topic>transformacion genetica</topic><topic>transformation genetique</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOLTES-RAK, E</creatorcontrib><creatorcontrib>KUSHNER, D. J</creatorcontrib><creatorcontrib>WILLIAMS, D. D</creatorcontrib><creatorcontrib>COLEMAN, J. 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J</au><au>WILLIAMS, D. D</au><au>COLEMAN, J. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>59</volume><issue>8</issue><spage>2404</spage><epage>2410</epage><pages>2404-2410</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter upstream of the cryIVB sequence. Replacement of the endogenous corresponding Bacillus sequences with the Synechococcus petF1 promoter, ribosome binding site, and initiation codon also resulted in increased expression of the cryIVB gene relative to the expression obtained with the Bacillus promoter alone but decreased expression relative to the expression achieved with the tandem array of the Bacillus and lacZ promoters. Synechococcus cells carrying plasmids in which the expression of the cryIVB gene was regulated by either the lacZ or the petF1 promoter were readily consumed by first-instar Culex restuans larvae and proved to be toxic for these organisms.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>7690220</pmid><doi>10.1128/aem.59.8.2404-2410.1993</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1993-08, Vol.59 (8), p.2404-2410 |
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| subjects | adn recombinado adn recombine bacillus thuringiensis Bacillus thuringiensis - genetics Bacillus thuringiensis israelensis Bacteria Bacterial Proteins - genetics Bacterial Toxins Biological and medical sciences Biotechnology Culex restuans Cyanobacteria - genetics cyanophyta endotoxinas endotoxine endotoxins Endotoxins - genetics Escherichia coli - genetics expresion genica expression des genes Freshwater Fundamental and applied biological sciences. Psychology gene Gene Expression Genes Genes, Bacterial Genetic engineering Genetic technics genetic transformation Genetic Vectors genetica genetics genetique Hemolysin Proteins insecticidas insecticide insecticides Insects larvae larvas larve Methods. Procedures. Technologies Modification of gene expression level Pest Control, Biological Plasmids - genetics Promoter Regions, Genetic recombinant dna RNA, Bacterial - genetics RNA, Messenger - genetics Synechococcus toxinas toxine toxins transformacion genetica transformation genetique Transformation, Genetic |
| title | Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942 |
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