Negative electrospray ionization mass spectrometry: a method for sequencing and determining linkage position in oligosaccharides from branched hemicelluloses

Xyloglucans of apple, tomato, bilberry and tamarind were hydrolyzed by commercial endo β‐1‐4‐D‐endoglucanase. The xylo‐gluco‐oligosaccharides (XylGos) released were separated on CarboPac PA 200 column in less than 15 min, and, after purification, they were structurally characterized by negative elec...

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Veröffentlicht in:	Journal of mass spectrometry. 2015-01, Vol.50 (1), p.247-264
Hauptverfasser:	Quéméner, Bernard, Vigouroux, Jacqueline, Rathahao, Estelle, Tabet, Jean Claude, Dimitrijevic, Aleksandra, Lahaye, Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrate Conformation Chromatography, Liquid - methods Cleavage endo β-1-4 endoglucanase Fragmentation Glucans - analysis Glucans - chemistry Glucans - isolation & purification Glucuronic Acid - chemistry hemicellulose HPAEC Ionization Isomerism Linkages Lycopersicon esculentum Malus - chemistry Mass spectrometry negative ESI-MS Oligosaccharides - analysis Oligosaccharides - chemistry Polysaccharides - analysis Polysaccharides - chemistry Quadrupoles Sequencing Spectra Spectrometry, Mass, Electrospray Ionization - methods Spectroscopy, Fourier Transform Infrared - instrumentation Tamarindus - chemistry Vaccinium myrtillus - chemistry Xylans - analysis Xylans - chemistry Xylans - isolation & purification xyloglucans
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