Tumor necrosis factor increases transcription of the heparin-binding epidermal growth factor-like growth factor gene in vascular endothelial cells
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified, potent vascular smooth muscle cell (SMC) mitogen of macrophage origin. To determine whether this gene is transcribed and regulated in vascular endothelial cells, we measured HB-EGF mRNA levels in human umbi...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-05, Vol.267 (14), p.9467-9469 |
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| container_issue | 14 |
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| container_title | The Journal of biological chemistry |
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| creator | YOSHIZUMI, M DOUREMBANA, S TEMIZER, D. H CAMBRIA, R. P QUERTERMOUS, T MU-EN LEE |
| description | Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified, potent vascular smooth muscle
cell (SMC) mitogen of macrophage origin. To determine whether this gene is transcribed and regulated in vascular endothelial
cells, we measured HB-EGF mRNA levels in human umbilical vein endothelial cells (HUVEC) by RNA blot analysis with an HB-EGF
cDNA probe. The base-line level of HB-EFG mRNA in HUVEC in culture was low. However, tumor necrosis factor-alpha (TNF-alpha)
and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the
gene by TNF-alpha was both dose- and time-dependent. In response to TNF-alpha, HB-EGF mRNA levels quickly increased and peaked
at 1 h, indicating that HB-EGF belongs to the family of immediate early genes. In nuclear run-off experiments, TNF-alpha increased
the rate of HB-EGF gene transcription by 3.2-fold. To our knowledge this is the first demonstration that the HB-EGF gene is
transcribed in vascular endothelial cells. The inducible transcription of this potent SMC mitogen gene in endothelial cells
suggests that HB-EGF may have an important role in the pathogenesis of atherosclerosis. |
| doi_str_mv | 10.1016/s0021-9258(19)50112-0 |
| format | Article |
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cell (SMC) mitogen of macrophage origin. To determine whether this gene is transcribed and regulated in vascular endothelial
cells, we measured HB-EGF mRNA levels in human umbilical vein endothelial cells (HUVEC) by RNA blot analysis with an HB-EGF
cDNA probe. The base-line level of HB-EFG mRNA in HUVEC in culture was low. However, tumor necrosis factor-alpha (TNF-alpha)
and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the
gene by TNF-alpha was both dose- and time-dependent. In response to TNF-alpha, HB-EGF mRNA levels quickly increased and peaked
at 1 h, indicating that HB-EGF belongs to the family of immediate early genes. In nuclear run-off experiments, TNF-alpha increased
the rate of HB-EGF gene transcription by 3.2-fold. To our knowledge this is the first demonstration that the HB-EGF gene is
transcribed in vascular endothelial cells. The inducible transcription of this potent SMC mitogen gene in endothelial cells
suggests that HB-EGF may have an important role in the pathogenesis of atherosclerosis.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(19)50112-0</identifier><identifier>PMID: 1577791</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Base Sequence ; Biological and medical sciences ; Cell physiology ; Cells, Cultured ; Cloning, Molecular ; DNA - genetics ; DNA - isolation & purification ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; Epidermal Growth Factor - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Heparin - genetics ; Heparin-binding EGF-like Growth Factor ; Humans ; Intercellular Signaling Peptides and Proteins ; Interferon-gamma - pharmacology ; Interleukin-1 - pharmacology ; Kinetics ; Molecular and cellular biology ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Recombinant Proteins - pharmacology ; Responses to growth factors, tumor promotors, other factors ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; RNA, Messenger - isolation & purification ; Tetradecanoylphorbol Acetate - pharmacology ; Transcription, Genetic - drug effects ; Tumor Necrosis Factor-alpha - pharmacology ; Umbilical Veins</subject><ispartof>The Journal of biological chemistry, 1992-05, Vol.267 (14), p.9467-9469</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-b1a4a8263440cf273be6998a492c474e153c30cd8add885f9559a2a9958c7f63</citedby><cites>FETCH-LOGICAL-c505t-b1a4a8263440cf273be6998a492c474e153c30cd8add885f9559a2a9958c7f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5325961$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1577791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOSHIZUMI, M</creatorcontrib><creatorcontrib>DOUREMBANA, S</creatorcontrib><creatorcontrib>TEMIZER, D. H</creatorcontrib><creatorcontrib>CAMBRIA, R. P</creatorcontrib><creatorcontrib>QUERTERMOUS, T</creatorcontrib><creatorcontrib>MU-EN LEE</creatorcontrib><title>Tumor necrosis factor increases transcription of the heparin-binding epidermal growth factor-like growth factor gene in vascular endothelial cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified, potent vascular smooth muscle
cell (SMC) mitogen of macrophage origin. To determine whether this gene is transcribed and regulated in vascular endothelial
cells, we measured HB-EGF mRNA levels in human umbilical vein endothelial cells (HUVEC) by RNA blot analysis with an HB-EGF
cDNA probe. The base-line level of HB-EFG mRNA in HUVEC in culture was low. However, tumor necrosis factor-alpha (TNF-alpha)
and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the
gene by TNF-alpha was both dose- and time-dependent. In response to TNF-alpha, HB-EGF mRNA levels quickly increased and peaked
at 1 h, indicating that HB-EGF belongs to the family of immediate early genes. In nuclear run-off experiments, TNF-alpha increased
the rate of HB-EGF gene transcription by 3.2-fold. To our knowledge this is the first demonstration that the HB-EGF gene is
transcribed in vascular endothelial cells. The inducible transcription of this potent SMC mitogen gene in endothelial cells
suggests that HB-EGF may have an important role in the pathogenesis of atherosclerosis.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Heparin - genetics</subject><subject>Heparin-binding EGF-like Growth Factor</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Interferon-gamma - pharmacology</subject><subject>Interleukin-1 - pharmacology</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Umbilical Veins</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1u1TAQhS1EVS6FR6hkCYRgEfBvYi-rij-pEgvugp3lOJMbQ2IHO6HiNfrEdbhXRfVmZM85x6NvELqk5D0ltP6QCWG00kyqt1S_k4RSVpEnaEeJ4hWX9MdTtHuQPEPPc_5JyhGanqNzKpum0XSH7vbrFBMO4FLMPuPeuqXcfXAJbIaMl2RDdsnPi48Bxx4vA-ABZpt8qFofOh8OGGbfQZrsiA8p3i7DKaYa_S94_IQPEKDE4z82u3W0CUPoYskcfXE7GMf8Ap31dszw8lQv0P7Tx_31l-rm2-ev11c3lZNELlVLrbCK1VwI4nrW8BZqrZUVmjnRCKCSO05cp2zXKSV7LaW2zGotlWv6ml-gN8fYOcXfK-TFTD5vA9gAcc2G1lIXnypCeRRuiHKC3szJTzb9NZSYbRXm-8bZbJwN1ebfKgwpvsvTB2s7QfffdWRf-q9P_YLCjn0B7Xx-kEnOpK432aujbPCH4dYnMK2PboDJsLoxVBgt6obfAzrUoFs</recordid><startdate>19920515</startdate><enddate>19920515</enddate><creator>YOSHIZUMI, M</creator><creator>DOUREMBANA, S</creator><creator>TEMIZER, D. H</creator><creator>CAMBRIA, R. P</creator><creator>QUERTERMOUS, T</creator><creator>MU-EN LEE</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19920515</creationdate><title>Tumor necrosis factor increases transcription of the heparin-binding epidermal growth factor-like growth factor gene in vascular endothelial cells</title><author>YOSHIZUMI, M ; DOUREMBANA, S ; TEMIZER, D. H ; CAMBRIA, R. P ; QUERTERMOUS, T ; MU-EN LEE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-b1a4a8263440cf273be6998a492c474e153c30cd8add885f9559a2a9958c7f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>Epidermal Growth Factor - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Heparin - genetics</topic><topic>Heparin-binding EGF-like Growth Factor</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Interferon-gamma - pharmacology</topic><topic>Interleukin-1 - pharmacology</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Oligodeoxyribonucleotides</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOSHIZUMI, M</creatorcontrib><creatorcontrib>DOUREMBANA, S</creatorcontrib><creatorcontrib>TEMIZER, D. H</creatorcontrib><creatorcontrib>CAMBRIA, R. P</creatorcontrib><creatorcontrib>QUERTERMOUS, T</creatorcontrib><creatorcontrib>MU-EN LEE</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOSHIZUMI, M</au><au>DOUREMBANA, S</au><au>TEMIZER, D. H</au><au>CAMBRIA, R. P</au><au>QUERTERMOUS, T</au><au>MU-EN LEE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor necrosis factor increases transcription of the heparin-binding epidermal growth factor-like growth factor gene in vascular endothelial cells</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1992-05-15</date><risdate>1992</risdate><volume>267</volume><issue>14</issue><spage>9467</spage><epage>9469</epage><pages>9467-9469</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified, potent vascular smooth muscle
cell (SMC) mitogen of macrophage origin. To determine whether this gene is transcribed and regulated in vascular endothelial
cells, we measured HB-EGF mRNA levels in human umbilical vein endothelial cells (HUVEC) by RNA blot analysis with an HB-EGF
cDNA probe. The base-line level of HB-EFG mRNA in HUVEC in culture was low. However, tumor necrosis factor-alpha (TNF-alpha)
and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the
gene by TNF-alpha was both dose- and time-dependent. In response to TNF-alpha, HB-EGF mRNA levels quickly increased and peaked
at 1 h, indicating that HB-EGF belongs to the family of immediate early genes. In nuclear run-off experiments, TNF-alpha increased
the rate of HB-EGF gene transcription by 3.2-fold. To our knowledge this is the first demonstration that the HB-EGF gene is
transcribed in vascular endothelial cells. The inducible transcription of this potent SMC mitogen gene in endothelial cells
suggests that HB-EGF may have an important role in the pathogenesis of atherosclerosis.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1577791</pmid><doi>10.1016/s0021-9258(19)50112-0</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1992-05, Vol.267 (14), p.9467-9469 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16598858 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Cell physiology Cells, Cultured Cloning, Molecular DNA - genetics DNA - isolation & purification Endothelium, Vascular - drug effects Endothelium, Vascular - physiology Epidermal Growth Factor - genetics Fundamental and applied biological sciences. Psychology Gene Library Heparin - genetics Heparin-binding EGF-like Growth Factor Humans Intercellular Signaling Peptides and Proteins Interferon-gamma - pharmacology Interleukin-1 - pharmacology Kinetics Molecular and cellular biology Molecular Sequence Data Oligodeoxyribonucleotides Recombinant Proteins - pharmacology Responses to growth factors, tumor promotors, other factors RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Messenger - isolation & purification Tetradecanoylphorbol Acetate - pharmacology Transcription, Genetic - drug effects Tumor Necrosis Factor-alpha - pharmacology Umbilical Veins |
| title | Tumor necrosis factor increases transcription of the heparin-binding epidermal growth factor-like growth factor gene in vascular endothelial cells |
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