A specific and reliable bioassay for the detection of femtomolar levels of human and murine tumor necrosis factors

A reliable, highly sensitive, cytolytic bioassay for the quantitation of both human and murine tumor necrosis factor (TNF) is described. The assay is 2–180-fold more sensitive than other currently described bio- or immunoassays (limits of detection: 500 fg/ml (29 fmol/1) human TNF-α, 200 fg/ml (12 f...

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Veröffentlicht in:	Journal of immunological methods 1991-10, Vol.143 (2), p.251-261
Hauptverfasser:	Branch, Donald R., Shah, Anjnaben, Guilbert, Larry J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioassay Biological and medical sciences Biological Assay - methods Cell Survival - drug effects Dactinomycin Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Lymphotoxin Mice Mitomycin Molecular immunology Recombinant Proteins - analysis Reproducibility of Results Sensitivity and Specificity Techniques Tumor Cells, Cultured Tumor necrosis factor Tumor Necrosis Factor-alpha - analysis
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