Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish
Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macroph...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2015-02, Vol.63 (7), p.2098-2105 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Ryu, Su-Jung Choi, Hyeon-Son Yoon, Kye-Yoon Lee, Ok-Hwan Kim, Kui-Jin Lee, Boo-Yong |
| description | Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent. |
| doi_str_mv | 10.1021/jf505894b |
| format | Article |
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In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf505894b</identifier><identifier>PMID: 25613688</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; anti-inflammatory activity ; anti-inflammatory agents ; Anti-Inflammatory Agents - administration & dosage ; Cyclooxygenase 2 - genetics ; Cyclooxygenase 2 - immunology ; Danio rerio ; enzyme immunoassays ; genes ; inducible nitric oxide synthase ; inflammation ; Inflammation - drug therapy ; Inflammation - genetics ; Inflammation - immunology ; interleukin-1beta ; interleukin-6 ; Interleukin-6 - genetics ; Interleukin-6 - immunology ; Iridoids - administration & dosage ; leaves ; lipopolysaccharides ; Lipopolysaccharides - adverse effects ; Lipopolysaccharides - immunology ; macrophages ; Macrophages - drug effects ; Macrophages - immunology ; Mice ; mitogen-activated protein kinase ; NF-kappa B - genetics ; NF-kappa B - immunology ; nitric oxide ; Olea - chemistry ; oleuropein ; olives ; phenolic compounds ; phosphorylation ; Plant Extracts - administration & dosage ; Plant Leaves - chemistry ; prostaglandin synthase ; Zebrafish</subject><ispartof>Journal of agricultural and food chemistry, 2015-02, Vol.63 (7), p.2098-2105</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a339t-dd6be9d41a2184f57f5391c59e4065c7df2fc18335a1db8ea4a67d490ee77d5e3</citedby><cites>FETCH-LOGICAL-a339t-dd6be9d41a2184f57f5391c59e4065c7df2fc18335a1db8ea4a67d490ee77d5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf505894b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf505894b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25613688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ryu, Su-Jung</creatorcontrib><creatorcontrib>Choi, Hyeon-Son</creatorcontrib><creatorcontrib>Yoon, Kye-Yoon</creatorcontrib><creatorcontrib>Lee, Ok-Hwan</creatorcontrib><creatorcontrib>Kim, Kui-Jin</creatorcontrib><creatorcontrib>Lee, Boo-Yong</creatorcontrib><title>Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent.</description><subject>Animals</subject><subject>anti-inflammatory activity</subject><subject>anti-inflammatory agents</subject><subject>Anti-Inflammatory Agents - administration & dosage</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase 2 - immunology</subject><subject>Danio rerio</subject><subject>enzyme immunoassays</subject><subject>genes</subject><subject>inducible nitric oxide synthase</subject><subject>inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Inflammation - genetics</subject><subject>Inflammation - immunology</subject><subject>interleukin-1beta</subject><subject>interleukin-6</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - immunology</subject><subject>Iridoids - administration & dosage</subject><subject>leaves</subject><subject>lipopolysaccharides</subject><subject>Lipopolysaccharides - adverse effects</subject><subject>Lipopolysaccharides - immunology</subject><subject>macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Mice</subject><subject>mitogen-activated protein kinase</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - immunology</subject><subject>nitric oxide</subject><subject>Olea - chemistry</subject><subject>oleuropein</subject><subject>olives</subject><subject>phenolic compounds</subject><subject>phosphorylation</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Leaves - chemistry</subject><subject>prostaglandin synthase</subject><subject>Zebrafish</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtLw0AQgBdRtFYP_gHNRdBD6kySTTZHKT4KhUprUbwsm-yspuTlbnPw35vS2pOngZlvXh9jFwgjhADvVoYDF2mUHbAB8gB8jigO2QD6oi94jCfs1LkVAAiewDE7CfpcGAsxYO-zkjrbtFTU3qJrW0vOkfOmLwt_UusuJ-1NalOqqlLrxv54c3JtU2-QvmF-_-YFcTRKvDGVpadq7X1QZpUp3NcZOzKqdHS-i0O2fHx4HT_709nTZHw_9VUYpmtf6zijVEeoAhSR4YnhYYo5TymCmOeJNoHJUYQhV6gzQSpScaKjFIiSRHMKh-xmO7e1zXdHbi2rwuX9OaqmpnMSYy4SCEX_8JDdbtHcNs5ZMrK1RaXsj0SQG5FyL7JnL3dju6wivSf_zPXA1RYwqpHq0xZOLhcBYAyAAQJuiOstoXInV01n697DP6t-ASgAgaU</recordid><startdate>20150225</startdate><enddate>20150225</enddate><creator>Ryu, Su-Jung</creator><creator>Choi, Hyeon-Son</creator><creator>Yoon, Kye-Yoon</creator><creator>Lee, Ok-Hwan</creator><creator>Kim, Kui-Jin</creator><creator>Lee, Boo-Yong</creator><general>American Chemical Society</general><general>American Chemical Society, Books and Journals Division</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150225</creationdate><title>Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish</title><author>Ryu, Su-Jung ; Choi, Hyeon-Son ; Yoon, Kye-Yoon ; Lee, Ok-Hwan ; Kim, Kui-Jin ; Lee, Boo-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a339t-dd6be9d41a2184f57f5391c59e4065c7df2fc18335a1db8ea4a67d490ee77d5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>anti-inflammatory activity</topic><topic>anti-inflammatory agents</topic><topic>Anti-Inflammatory Agents - administration & dosage</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase 2 - immunology</topic><topic>Danio rerio</topic><topic>enzyme immunoassays</topic><topic>genes</topic><topic>inducible nitric oxide synthase</topic><topic>inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Inflammation - genetics</topic><topic>Inflammation - immunology</topic><topic>interleukin-1beta</topic><topic>interleukin-6</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - immunology</topic><topic>Iridoids - administration & dosage</topic><topic>leaves</topic><topic>lipopolysaccharides</topic><topic>Lipopolysaccharides - adverse effects</topic><topic>Lipopolysaccharides - immunology</topic><topic>macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Mice</topic><topic>mitogen-activated protein kinase</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - immunology</topic><topic>nitric oxide</topic><topic>Olea - chemistry</topic><topic>oleuropein</topic><topic>olives</topic><topic>phenolic compounds</topic><topic>phosphorylation</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Leaves - chemistry</topic><topic>prostaglandin synthase</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryu, Su-Jung</creatorcontrib><creatorcontrib>Choi, Hyeon-Son</creatorcontrib><creatorcontrib>Yoon, Kye-Yoon</creatorcontrib><creatorcontrib>Lee, Ok-Hwan</creatorcontrib><creatorcontrib>Kim, Kui-Jin</creatorcontrib><creatorcontrib>Lee, Boo-Yong</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryu, Su-Jung</au><au>Choi, Hyeon-Son</au><au>Yoon, Kye-Yoon</au><au>Lee, Ok-Hwan</au><au>Kim, Kui-Jin</au><au>Lee, Boo-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2015-02-25</date><risdate>2015</risdate><volume>63</volume><issue>7</issue><spage>2098</spage><epage>2105</epage><pages>2098-2105</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25613688</pmid><doi>10.1021/jf505894b</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals anti-inflammatory activity anti-inflammatory agents Anti-Inflammatory Agents - administration & dosage Cyclooxygenase 2 - genetics Cyclooxygenase 2 - immunology Danio rerio enzyme immunoassays genes inducible nitric oxide synthase inflammation Inflammation - drug therapy Inflammation - genetics Inflammation - immunology interleukin-1beta interleukin-6 Interleukin-6 - genetics Interleukin-6 - immunology Iridoids - administration & dosage leaves lipopolysaccharides Lipopolysaccharides - adverse effects Lipopolysaccharides - immunology macrophages Macrophages - drug effects Macrophages - immunology Mice mitogen-activated protein kinase NF-kappa B - genetics NF-kappa B - immunology nitric oxide Olea - chemistry oleuropein olives phenolic compounds phosphorylation Plant Extracts - administration & dosage Plant Leaves - chemistry prostaglandin synthase Zebrafish |
| title | Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish |
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