Cloning and Sequencing of Thiol-Specific Antioxidant from Mammalian Brain: Alkyl Hydroperoxide Reductase and Thiol-Specific Antioxidant Define a Large Family of Antioxidant Enzymes
A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-07, Vol.91 (15), p.7017-7021 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Chae, Ho Zoon Robison, Keith Poole, Leslie B. Church, George Storz, Gisela Rhee, Sue Goo |
| description | A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of >25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members. |
| doi_str_mv | 10.1073/pnas.91.15.7017 |
| format | Article |
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The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of >25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.15.7017</identifier><identifier>PMID: 8041738</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; ANTIOXIDANTS ; Antioxidants - chemistry ; Antioxidants - metabolism ; Base Sequence ; Biochemistry ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Brain ; Brain - enzymology ; Cattle ; Cloning, Molecular ; Complementary DNA ; Deoxyribonucleic acid ; DNA ; DNA SEQUENCING ; DNA-CLONING ; Enzymes ; Family members ; GENES ; Humans ; Immunity (Disease) ; Molecular Sequence Data ; Open reading frames ; OXIDOREDUCTASES ; Oxidoreductases - chemistry ; Oxidoreductases - metabolism ; Peroxidases ; Peroxides ; Peroxiredoxins ; Proteins ; Proteins - chemistry ; Proteins - metabolism ; Rats ; Rodents ; Sequence Homology, Amino Acid ; Sequencing ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-07, Vol.91 (15), p.7017-7021</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jul 19, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c611t-eec410adba5e54906359885177565c64619652c6ddf46e54b149b643f79cdc463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2365210$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2365210$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8041738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/86535$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Chae, Ho Zoon</creatorcontrib><creatorcontrib>Robison, Keith</creatorcontrib><creatorcontrib>Poole, Leslie B.</creatorcontrib><creatorcontrib>Church, George</creatorcontrib><creatorcontrib>Storz, Gisela</creatorcontrib><creatorcontrib>Rhee, Sue Goo</creatorcontrib><title>Cloning and Sequencing of Thiol-Specific Antioxidant from Mammalian Brain: Alkyl Hydroperoxide Reductase and Thiol-Specific Antioxidant Define a Large Family of Antioxidant Enzymes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of >25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>ANTIOXIDANTS</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - metabolism</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Brain</subject><subject>Brain - enzymology</subject><subject>Cattle</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA SEQUENCING</subject><subject>DNA-CLONING</subject><subject>Enzymes</subject><subject>Family members</subject><subject>GENES</subject><subject>Humans</subject><subject>Immunity (Disease)</subject><subject>Molecular Sequence Data</subject><subject>Open reading frames</subject><subject>OXIDOREDUCTASES</subject><subject>Oxidoreductases - chemistry</subject><subject>Oxidoreductases - metabolism</subject><subject>Peroxidases</subject><subject>Peroxides</subject><subject>Peroxiredoxins</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rodents</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequencing</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9v0zAcxSMEGmNw5gLI4gCndHb8IzHiUsrGkIqQ2DhbruO0Lo5dbAet_F38gTi0VBuHcbKs93nP9tevKJ4iOEGwxqcbJ-OEowmikxqi-l5xjCBHJSMc3i-OIazqsiEVeVg8inENIeS0gUfFUQMJqnFzXPyaWe-MWwLpWnCpvw_aqXHrO3C1Mt6WlxutTGcUmLpk_LVppUugC74Hn2TfS2ukA--CNO4NmNpvWwsutm3wGx1GVoMvuh1UklH_OeCOyPe6My5TYC7DUoNz2Ru7Ha9xEzpzP7e9jo-LB520UT_ZryfF1_Ozq9lFOf_84eNsOi8VQyiVWiuCoGwXkmqaB8Iw5U1DUV1TRhUjDHFGK8XatiMsEwtE-IIR3NVctYowfFK83eVuhkWvW6VdCtKKTTC9DFvhpRG3FWdWYul_CEJwxbMd7Ow-JiOiMkmrlfLOaZVEwyimGXm1PyH4PPuYRG-i0tZKp_0QRc1YhXAF_wsiRhsKUZXBl_-Aaz8El8ckKpijaszrDJ3uIBV8jEF3hzchKMZeibFXgiOBqBh7lR3Pb47iwO-LlPUXe300_lVvBby-ExDdYG3S1ymTz3bkOiYfDmiF82chiH8DEjXtTw</recordid><startdate>19940719</startdate><enddate>19940719</enddate><creator>Chae, Ho Zoon</creator><creator>Robison, Keith</creator><creator>Poole, Leslie B.</creator><creator>Church, George</creator><creator>Storz, Gisela</creator><creator>Rhee, Sue Goo</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19940719</creationdate><title>Cloning and Sequencing of Thiol-Specific Antioxidant from Mammalian Brain: Alkyl Hydroperoxide Reductase and Thiol-Specific Antioxidant Define a Large Family of Antioxidant Enzymes</title><author>Chae, Ho Zoon ; Robison, Keith ; Poole, Leslie B. ; Church, George ; Storz, Gisela ; Rhee, Sue Goo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c611t-eec410adba5e54906359885177565c64619652c6ddf46e54b149b643f79cdc463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>ANTIOXIDANTS</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - metabolism</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Brain</topic><topic>Brain - enzymology</topic><topic>Cattle</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA SEQUENCING</topic><topic>DNA-CLONING</topic><topic>Enzymes</topic><topic>Family members</topic><topic>GENES</topic><topic>Humans</topic><topic>Immunity (Disease)</topic><topic>Molecular Sequence Data</topic><topic>Open reading frames</topic><topic>OXIDOREDUCTASES</topic><topic>Oxidoreductases - chemistry</topic><topic>Oxidoreductases - metabolism</topic><topic>Peroxidases</topic><topic>Peroxides</topic><topic>Peroxiredoxins</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rodents</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequencing</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chae, Ho Zoon</creatorcontrib><creatorcontrib>Robison, Keith</creatorcontrib><creatorcontrib>Poole, Leslie B.</creatorcontrib><creatorcontrib>Church, George</creatorcontrib><creatorcontrib>Storz, Gisela</creatorcontrib><creatorcontrib>Rhee, Sue Goo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chae, Ho Zoon</au><au>Robison, Keith</au><au>Poole, Leslie B.</au><au>Church, George</au><au>Storz, Gisela</au><au>Rhee, Sue Goo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Sequencing of Thiol-Specific Antioxidant from Mammalian Brain: Alkyl Hydroperoxide Reductase and Thiol-Specific Antioxidant Define a Large Family of Antioxidant Enzymes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-07-19</date><risdate>1994</risdate><volume>91</volume><issue>15</issue><spage>7017</spage><epage>7021</epage><pages>7017-7021</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of >25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8041738</pmid><doi>10.1073/pnas.91.15.7017</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-07, Vol.91 (15), p.7017-7021 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Animals ANTIOXIDANTS Antioxidants - chemistry Antioxidants - metabolism Base Sequence Biochemistry BIOLOGY AND MEDICINE, BASIC STUDIES Brain Brain - enzymology Cattle Cloning, Molecular Complementary DNA Deoxyribonucleic acid DNA DNA SEQUENCING DNA-CLONING Enzymes Family members GENES Humans Immunity (Disease) Molecular Sequence Data Open reading frames OXIDOREDUCTASES Oxidoreductases - chemistry Oxidoreductases - metabolism Peroxidases Peroxides Peroxiredoxins Proteins Proteins - chemistry Proteins - metabolism Rats Rodents Sequence Homology, Amino Acid Sequencing Ungulates |
| title | Cloning and Sequencing of Thiol-Specific Antioxidant from Mammalian Brain: Alkyl Hydroperoxide Reductase and Thiol-Specific Antioxidant Define a Large Family of Antioxidant Enzymes |
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