Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos

•Trophectoderm (TE) cells with stem-like properties were isolated from pig blastocysts.•TE cells cultured in vitro express gene and protein markers of trophoblast function.•FBS induces differentiation but a serum-free, defined medium promotes self-renewal.•Cells are amenable to genetic modification...

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Veröffentlicht in:	Animal reproduction science 2015-03, Vol.154, p.128-141
Hauptverfasser:	Suasnavas, Edison A., Heywood, Sierra, Ward, Anika, Cox, Lindsay, O'Grady, Merecedes, Zhao, Yuanfeng, Gilbert, Lacey, Isom, S. Clay
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals DNA Methylation Embryo Culture Techniques - veterinary Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Gene expression Gene Expression Regulation, Developmental - physiology Genes, Developmental - physiology RNA, Messenger - genetics RNA, Messenger - metabolism Swine - embryology Transfection Trophectoderm differentiation Trophoblast stem cells Trophoblasts - cytology
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