Fluorescent activated cell sorting: An effective approach to study dendritic cell subsets in human atherosclerotic plaques
Different immune cell types are present within atherosclerotic plaques. Dendritic cells (DC) are of special interest, since they are considered as the ‘center of the immuniverse’. Identifying inflammatory DC subtypes within plaques is important for a better understanding of the lesion pathogenesis a...
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| Veröffentlicht in: | Journal of immunological methods 2015-02, Vol.417, p.76-85 |
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| creator | Van Brussel, Ilse Ammi, Rachid Rombouts, Miche Cools, Nathalie Vercauteren, Sven R. De Roover, Dominique Hendriks, Jeroen M.H. Lauwers, Patrick Van Schil, Paul E. Schrijvers, Dorien M. |
| description | Different immune cell types are present within atherosclerotic plaques. Dendritic cells (DC) are of special interest, since they are considered as the ‘center of the immuniverse’. Identifying inflammatory DC subtypes within plaques is important for a better understanding of the lesion pathogenesis and pinpoints their contribution to the atherosclerotic process. We have developed a flow cytometry-based method to characterize and isolate different DC subsets (i.e. CD11b+, Clec9A+ and CD16+ conventional (c)DC and CD123+ plasmacytoid (p)DC) in human atherosclerotic plaques. We revealed a predominance of pro-inflammatory CD11b+ DC in advanced human lesions, whereas atheroprotective Clec9A+ DC were almost absent. CD123+ pDC and CD16+ DC were also detectable in plaques. Remarkably, plaques from distinct anatomical locations exhibited different cellular compositions: femoral plaques contained less CD11b+ and Clec9A+ DC than carotid plaques. Twice as many monocytes/macrophages were observed compared to DC. Moreover, relative amounts of T cells/B cells/NK cells were 6 times as high as DC numbers.
For the first time, fluorescent activated cell sorting analysis of DC subsets in human plaques indicated a predominance of CD11b+ cDC, in comparison with other DC subsets. Isolation of the different subsets will facilitate detailed functional analysis and may have significant implications for tailoring appropriate therapy.
•Understanding the origin/function of DC subsets that control atherogenesis is needed.•It can open up strategies to interfere with DC subset differentiation and functions for developing therapeutic targets.•FACS allowed us to characterize the presence of specialized DC subsets in human atherosclerotic lesions.•FACS analysis of carotid and femoral arteries revealed a predominance of the CD11bpos subset.•Also, different locations in the vascular tree show differences in the accumulation of specialized DC subsets. |
| doi_str_mv | 10.1016/j.jim.2014.12.010 |
| format | Article |
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For the first time, fluorescent activated cell sorting analysis of DC subsets in human plaques indicated a predominance of CD11b+ cDC, in comparison with other DC subsets. Isolation of the different subsets will facilitate detailed functional analysis and may have significant implications for tailoring appropriate therapy.
•Understanding the origin/function of DC subsets that control atherogenesis is needed.•It can open up strategies to interfere with DC subset differentiation and functions for developing therapeutic targets.•FACS allowed us to characterize the presence of specialized DC subsets in human atherosclerotic lesions.•FACS analysis of carotid and femoral arteries revealed a predominance of the CD11bpos subset.•Also, different locations in the vascular tree show differences in the accumulation of specialized DC subsets.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2014.12.010</identifier><identifier>PMID: 25527343</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aged ; Aged, 80 and over ; Atherosclerosis ; Atherosclerosis - immunology ; B-Lymphocytes - cytology ; CD11b Antigen - metabolism ; Cell Separation - methods ; Dendritic cells ; Dendritic Cells - immunology ; Female ; Flow Cytometry - methods ; Fluorescence activated cell sorting ; GPI-Linked Proteins - metabolism ; Humans ; Inflammation - immunology ; Interleukin-3 Receptor alpha Subunit - metabolism ; Killer Cells, Natural - cytology ; Lectins, C-Type - metabolism ; Macrophages - cytology ; Male ; Middle Aged ; Monocytes - cytology ; Plaque, Atherosclerotic - immunology ; Plaques ; Receptors, IgG - metabolism ; Receptors, Mitogen - metabolism ; T-Lymphocytes - cytology</subject><ispartof>Journal of immunological methods, 2015-02, Vol.417, p.76-85</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-e83e8be109484ef59567f4a200a1224bcd07fdbee5aa09abc01cef100666b9973</citedby><cites>FETCH-LOGICAL-c466t-e83e8be109484ef59567f4a200a1224bcd07fdbee5aa09abc01cef100666b9973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175914003639$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25527343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Brussel, Ilse</creatorcontrib><creatorcontrib>Ammi, Rachid</creatorcontrib><creatorcontrib>Rombouts, Miche</creatorcontrib><creatorcontrib>Cools, Nathalie</creatorcontrib><creatorcontrib>Vercauteren, Sven R.</creatorcontrib><creatorcontrib>De Roover, Dominique</creatorcontrib><creatorcontrib>Hendriks, Jeroen M.H.</creatorcontrib><creatorcontrib>Lauwers, Patrick</creatorcontrib><creatorcontrib>Van Schil, Paul E.</creatorcontrib><creatorcontrib>Schrijvers, Dorien M.</creatorcontrib><title>Fluorescent activated cell sorting: An effective approach to study dendritic cell subsets in human atherosclerotic plaques</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Different immune cell types are present within atherosclerotic plaques. Dendritic cells (DC) are of special interest, since they are considered as the ‘center of the immuniverse’. Identifying inflammatory DC subtypes within plaques is important for a better understanding of the lesion pathogenesis and pinpoints their contribution to the atherosclerotic process. We have developed a flow cytometry-based method to characterize and isolate different DC subsets (i.e. CD11b+, Clec9A+ and CD16+ conventional (c)DC and CD123+ plasmacytoid (p)DC) in human atherosclerotic plaques. We revealed a predominance of pro-inflammatory CD11b+ DC in advanced human lesions, whereas atheroprotective Clec9A+ DC were almost absent. CD123+ pDC and CD16+ DC were also detectable in plaques. Remarkably, plaques from distinct anatomical locations exhibited different cellular compositions: femoral plaques contained less CD11b+ and Clec9A+ DC than carotid plaques. Twice as many monocytes/macrophages were observed compared to DC. Moreover, relative amounts of T cells/B cells/NK cells were 6 times as high as DC numbers.
For the first time, fluorescent activated cell sorting analysis of DC subsets in human plaques indicated a predominance of CD11b+ cDC, in comparison with other DC subsets. Isolation of the different subsets will facilitate detailed functional analysis and may have significant implications for tailoring appropriate therapy.
•Understanding the origin/function of DC subsets that control atherogenesis is needed.•It can open up strategies to interfere with DC subset differentiation and functions for developing therapeutic targets.•FACS allowed us to characterize the presence of specialized DC subsets in human atherosclerotic lesions.•FACS analysis of carotid and femoral arteries revealed a predominance of the CD11bpos subset.•Also, different locations in the vascular tree show differences in the accumulation of specialized DC subsets.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Atherosclerosis</subject><subject>Atherosclerosis - immunology</subject><subject>B-Lymphocytes - cytology</subject><subject>CD11b Antigen - metabolism</subject><subject>Cell Separation - methods</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Female</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescence activated cell sorting</subject><subject>GPI-Linked Proteins - metabolism</subject><subject>Humans</subject><subject>Inflammation - immunology</subject><subject>Interleukin-3 Receptor alpha Subunit - metabolism</subject><subject>Killer Cells, Natural - cytology</subject><subject>Lectins, C-Type - metabolism</subject><subject>Macrophages - cytology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monocytes - cytology</subject><subject>Plaque, Atherosclerotic - immunology</subject><subject>Plaques</subject><subject>Receptors, IgG - metabolism</subject><subject>Receptors, Mitogen - metabolism</subject><subject>T-Lymphocytes - cytology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1O5DAURi20CGaBB6BZuaRJuHZiJ4EKIWYXCYkGasuxbxiP8jNrO0jw9DiaWcpt7MLnu_7uIeSSQc6AyettvnVDzoGVOeM5MDgiK1ZXPKsaED_ICoDzjFWiOSU_Q9gCJETCCTnlQvCqKIsV-Vz38-QxGBwj1Sa6dx3RUoN9T8PkoxvfbujdSLHrcHlFqnc7P2mzoXGiIc72g1ocrXfRmUNsbgPGQN1IN_OgR6rjBv0UTJ_Ohdr1-u-M4Zwcd7oPeHG4z8jr-uHl_k_29Pz78f7uKTOllDHDusC6RQZNWZfYiUbIqis1B9CM87I1FqrOtohCa2h0a4AZ7BiAlLJtmqo4I1f7uan38m9UgwtLUz3iNAfFpKhLXhSVSCjboyb1DR47tfNu0P5DMVCLcrVVSblalCvGVfKZMr8O4-d2QPud-Oc4Abd7ANOS7w69CsbhaNA6n5wqO7n_jP8CmhKUKw</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Van Brussel, Ilse</creator><creator>Ammi, Rachid</creator><creator>Rombouts, Miche</creator><creator>Cools, Nathalie</creator><creator>Vercauteren, Sven R.</creator><creator>De Roover, Dominique</creator><creator>Hendriks, Jeroen M.H.</creator><creator>Lauwers, Patrick</creator><creator>Van Schil, Paul E.</creator><creator>Schrijvers, Dorien M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150201</creationdate><title>Fluorescent activated cell sorting: An effective approach to study dendritic cell subsets in human atherosclerotic plaques</title><author>Van Brussel, Ilse ; Ammi, Rachid ; Rombouts, Miche ; Cools, Nathalie ; Vercauteren, Sven R. ; De Roover, Dominique ; Hendriks, Jeroen M.H. ; Lauwers, Patrick ; Van Schil, Paul E. ; Schrijvers, Dorien M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-e83e8be109484ef59567f4a200a1224bcd07fdbee5aa09abc01cef100666b9973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Atherosclerosis</topic><topic>Atherosclerosis - immunology</topic><topic>B-Lymphocytes - cytology</topic><topic>CD11b Antigen - metabolism</topic><topic>Cell Separation - methods</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Female</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescence activated cell sorting</topic><topic>GPI-Linked Proteins - metabolism</topic><topic>Humans</topic><topic>Inflammation - immunology</topic><topic>Interleukin-3 Receptor alpha Subunit - metabolism</topic><topic>Killer Cells, Natural - cytology</topic><topic>Lectins, C-Type - metabolism</topic><topic>Macrophages - cytology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Monocytes - cytology</topic><topic>Plaque, Atherosclerotic - immunology</topic><topic>Plaques</topic><topic>Receptors, IgG - metabolism</topic><topic>Receptors, Mitogen - metabolism</topic><topic>T-Lymphocytes - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Brussel, Ilse</creatorcontrib><creatorcontrib>Ammi, Rachid</creatorcontrib><creatorcontrib>Rombouts, Miche</creatorcontrib><creatorcontrib>Cools, Nathalie</creatorcontrib><creatorcontrib>Vercauteren, Sven R.</creatorcontrib><creatorcontrib>De Roover, Dominique</creatorcontrib><creatorcontrib>Hendriks, Jeroen M.H.</creatorcontrib><creatorcontrib>Lauwers, Patrick</creatorcontrib><creatorcontrib>Van Schil, Paul E.</creatorcontrib><creatorcontrib>Schrijvers, Dorien M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Brussel, Ilse</au><au>Ammi, Rachid</au><au>Rombouts, Miche</au><au>Cools, Nathalie</au><au>Vercauteren, Sven R.</au><au>De Roover, Dominique</au><au>Hendriks, Jeroen M.H.</au><au>Lauwers, Patrick</au><au>Van Schil, Paul E.</au><au>Schrijvers, Dorien M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent activated cell sorting: An effective approach to study dendritic cell subsets in human atherosclerotic plaques</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>417</volume><spage>76</spage><epage>85</epage><pages>76-85</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>Different immune cell types are present within atherosclerotic plaques. Dendritic cells (DC) are of special interest, since they are considered as the ‘center of the immuniverse’. Identifying inflammatory DC subtypes within plaques is important for a better understanding of the lesion pathogenesis and pinpoints their contribution to the atherosclerotic process. We have developed a flow cytometry-based method to characterize and isolate different DC subsets (i.e. CD11b+, Clec9A+ and CD16+ conventional (c)DC and CD123+ plasmacytoid (p)DC) in human atherosclerotic plaques. We revealed a predominance of pro-inflammatory CD11b+ DC in advanced human lesions, whereas atheroprotective Clec9A+ DC were almost absent. CD123+ pDC and CD16+ DC were also detectable in plaques. Remarkably, plaques from distinct anatomical locations exhibited different cellular compositions: femoral plaques contained less CD11b+ and Clec9A+ DC than carotid plaques. Twice as many monocytes/macrophages were observed compared to DC. Moreover, relative amounts of T cells/B cells/NK cells were 6 times as high as DC numbers.
For the first time, fluorescent activated cell sorting analysis of DC subsets in human plaques indicated a predominance of CD11b+ cDC, in comparison with other DC subsets. Isolation of the different subsets will facilitate detailed functional analysis and may have significant implications for tailoring appropriate therapy.
•Understanding the origin/function of DC subsets that control atherogenesis is needed.•It can open up strategies to interfere with DC subset differentiation and functions for developing therapeutic targets.•FACS allowed us to characterize the presence of specialized DC subsets in human atherosclerotic lesions.•FACS analysis of carotid and femoral arteries revealed a predominance of the CD11bpos subset.•Also, different locations in the vascular tree show differences in the accumulation of specialized DC subsets.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25527343</pmid><doi>10.1016/j.jim.2014.12.010</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Aged, 80 and over Atherosclerosis Atherosclerosis - immunology B-Lymphocytes - cytology CD11b Antigen - metabolism Cell Separation - methods Dendritic cells Dendritic Cells - immunology Female Flow Cytometry - methods Fluorescence activated cell sorting GPI-Linked Proteins - metabolism Humans Inflammation - immunology Interleukin-3 Receptor alpha Subunit - metabolism Killer Cells, Natural - cytology Lectins, C-Type - metabolism Macrophages - cytology Male Middle Aged Monocytes - cytology Plaque, Atherosclerotic - immunology Plaques Receptors, IgG - metabolism Receptors, Mitogen - metabolism T-Lymphocytes - cytology |
| title | Fluorescent activated cell sorting: An effective approach to study dendritic cell subsets in human atherosclerotic plaques |
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