Knockout of leucine aminopeptidase in Toxoplasma gondii using CRISPR/Cas9

[Display omitted] •We used CRISPR/Cas9 genome editing to delete leucine aminopeptidase (LAP) in Toxoplasma gondii.•The resultant strains were subjected to functional assays.•Our data proved that LAP is not essential, but plays important roles in growth, invasion and virulence.•We concluded that LAP...

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Veröffentlicht in:	International journal for parasitology 2015-02, Vol.45 (2-3), p.141-148
Hauptverfasser:	Zheng, Jun, Jia, Honglin, Zheng, Yonghui
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western Cell Adhesion Cercopithecus aethiops CRISP/Cas9 CRISPR-Cas Systems Endocytosis Gene Deletion Gene Expression Profiling Gene Knockout Techniques - methods Genetic Complementation Test Growth Invasion Leucine aminopeptidase Leucyl Aminopeptidase - genetics Mice, Inbred BALB C Survival Analysis Toxoplasma - enzymology Toxoplasma - genetics Toxoplasma - physiology Toxoplasma gondii Toxoplasmosis, Animal Transfection Vero Cells
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