Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae
A cDNA copy of the carboxymethylcellulase (CMCase 1) gene, CMC1, of the yeast Cryptococcus flavus was expressed in Saccharomyces cerevisiae under the control of the glyceraldehyde-3-phosphate dehydrogenase gene ( GAP) promoter derived from S. cerevisiae. In glucose medium, extracellular production o...
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| Veröffentlicht in: | Journal of fermentation and bioengineering 1994-01, Vol.77 (3), p.235-238 |
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| container_title | Journal of fermentation and bioengineering |
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| creator | Hatano, Takushi Komura, Tsunehiro Cui, Zhifeng Mochizuki, Daisuke Miyakawa, Tokichi Matsuzaki, Hiroaki Fukui, Sakuzo |
| description | A cDNA copy of the carboxymethylcellulase (CMCase 1) gene,
CMC1, of the yeast
Cryptococcus flavus was expressed in
Saccharomyces cerevisiae under the control of the glyceraldehyde-3-phosphate dehydrogenase gene (
GAP) promoter derived from
S. cerevisiae. In glucose medium, extracellular production of a large amount of CMCase 1 was achieved by
S. cerevisiae cells transformed with YEp-plasmid carrying
CMC1. Two major proteins (36 kDa and 34 kDa) secreted in the medium both had CMCase activity and immuno-responsibility to anti-CMCase 1-antibody. From an analysis of amino acid sequences, it was found that the 36 kDa protein is a pro-CMCase 1 having an N-terminal residue, Ala, the 19th amino acid of the peptide deduced from the nucleotide sequence of
CMC1 on the cDNA, and that the 34 kDa protein is a mature CMCase 1. Alteration of the cell morphology of
S. cerevisiae was induced by transformation with YEp-plasmid carrying
CMC1. |
| doi_str_mv | 10.1016/0922-338X(94)90225-9 |
| format | Article |
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CMC1, of the yeast
Cryptococcus flavus was expressed in
Saccharomyces cerevisiae under the control of the glyceraldehyde-3-phosphate dehydrogenase gene (
GAP) promoter derived from
S. cerevisiae. In glucose medium, extracellular production of a large amount of CMCase 1 was achieved by
S. cerevisiae cells transformed with YEp-plasmid carrying
CMC1. Two major proteins (36 kDa and 34 kDa) secreted in the medium both had CMCase activity and immuno-responsibility to anti-CMCase 1-antibody. From an analysis of amino acid sequences, it was found that the 36 kDa protein is a pro-CMCase 1 having an N-terminal residue, Ala, the 19th amino acid of the peptide deduced from the nucleotide sequence of
CMC1 on the cDNA, and that the 34 kDa protein is a mature CMCase 1. Alteration of the cell morphology of
S. cerevisiae was induced by transformation with YEp-plasmid carrying
CMC1.</description><identifier>ISSN: 0922-338X</identifier><identifier>DOI: 10.1016/0922-338X(94)90225-9</identifier><identifier>CODEN: JFBIEX</identifier><language>eng</language><publisher>Osaka: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biotechnology ; CARBOXIMETILCELULOSA ; CARBOXYMETHYLCELLULOSE ; CITOLOGIA ; CRYPTOCOCCUS (CHAMPIGNON) ; CRYPTOCOCCUS (HONGO) ; CYTOLOGIE ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; GLICERALDEHIDO 3 FOSF DESHIDROG ; GLICOSIDASAS ; GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO ; GLYCOSIDASE ; Methods. Procedures. Technologies ; Modification of gene expression level ; PLASMIDE ; PLASMIDIOS ; REPONSE IMMUNITAIRE ; RESPUESTA INMUNOLOGICA ; SACCHAROMYCES CEREVISIAE</subject><ispartof>Journal of fermentation and bioengineering, 1994-01, Vol.77 (3), p.235-238</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-5cba63397073272828a3ffed6c2607a21095c986debaf982d613b41cd6c795c03</citedby><cites>FETCH-LOGICAL-c449t-5cba63397073272828a3ffed6c2607a21095c986debaf982d613b41cd6c795c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4048102$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hatano, Takushi</creatorcontrib><creatorcontrib>Komura, Tsunehiro</creatorcontrib><creatorcontrib>Cui, Zhifeng</creatorcontrib><creatorcontrib>Mochizuki, Daisuke</creatorcontrib><creatorcontrib>Miyakawa, Tokichi</creatorcontrib><creatorcontrib>Matsuzaki, Hiroaki</creatorcontrib><creatorcontrib>Fukui, Sakuzo</creatorcontrib><title>Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae</title><title>Journal of fermentation and bioengineering</title><description>A cDNA copy of the carboxymethylcellulase (CMCase 1) gene,
CMC1, of the yeast
Cryptococcus flavus was expressed in
Saccharomyces cerevisiae under the control of the glyceraldehyde-3-phosphate dehydrogenase gene (
GAP) promoter derived from
S. cerevisiae. In glucose medium, extracellular production of a large amount of CMCase 1 was achieved by
S. cerevisiae cells transformed with YEp-plasmid carrying
CMC1. Two major proteins (36 kDa and 34 kDa) secreted in the medium both had CMCase activity and immuno-responsibility to anti-CMCase 1-antibody. From an analysis of amino acid sequences, it was found that the 36 kDa protein is a pro-CMCase 1 having an N-terminal residue, Ala, the 19th amino acid of the peptide deduced from the nucleotide sequence of
CMC1 on the cDNA, and that the 34 kDa protein is a mature CMCase 1. Alteration of the cell morphology of
S. cerevisiae was induced by transformation with YEp-plasmid carrying
CMC1.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CARBOXIMETILCELULOSA</subject><subject>CARBOXYMETHYLCELLULOSE</subject><subject>CITOLOGIA</subject><subject>CRYPTOCOCCUS (CHAMPIGNON)</subject><subject>CRYPTOCOCCUS (HONGO)</subject><subject>CYTOLOGIE</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GLICERALDEHIDO 3 FOSF DESHIDROG</subject><subject>GLICOSIDASAS</subject><subject>GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO</subject><subject>GLYCOSIDASE</subject><subject>Methods. Procedures. Technologies</subject><subject>Modification of gene expression level</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>REPONSE IMMUNITAIRE</subject><subject>RESPUESTA INMUNOLOGICA</subject><subject>SACCHAROMYCES CEREVISIAE</subject><issn>0922-338X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rFEEQhuegYFz9A8FDH0QUMqa_5qMvggwxKgkKKnhramuqsy2z05vu2SXz79OTDTl6Kqh66qXqKYo3gn8UXNTn3EhZKtX-fW_0B8OlrErzrDh5ar8oXqb0j3OuuOQnhb-420VKyYeRBcemDTGEuA5385amzTwgDcN-gETshkY6Y911J86Yi2HLujjvpoABcZ-YG-CQix_ZL0DcQAZmpMSQIh188kCviucOhkSvH-uq-PPl4nf3tbz6cfmt-3xVotZmKitcQ62UaXijZCNb2YJyjvoaZc0bkIKbCk1b97QGZ1rZ10KttcAMNHnC1ap4d8zdxXC7pzTZrU_LGzBS2Ccr6qpVKqevCn0EMYaUIjm7i34LcbaC28WlXaTZRZo12j64tCavvX3Mh4QwuAgj-vS0q7luBZcZOz1iDoKFm5iR7z-N5o2ulhs_HYeURRw8RZvQ04jU-0g42T74_x9xD_jrk1s</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Hatano, Takushi</creator><creator>Komura, Tsunehiro</creator><creator>Cui, Zhifeng</creator><creator>Mochizuki, Daisuke</creator><creator>Miyakawa, Tokichi</creator><creator>Matsuzaki, Hiroaki</creator><creator>Fukui, Sakuzo</creator><general>Elsevier B.V</general><general>Society for Fermentation and Bioengineering</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope></search><sort><creationdate>19940101</creationdate><title>Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae</title><author>Hatano, Takushi ; Komura, Tsunehiro ; Cui, Zhifeng ; Mochizuki, Daisuke ; Miyakawa, Tokichi ; Matsuzaki, Hiroaki ; Fukui, Sakuzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-5cba63397073272828a3ffed6c2607a21095c986debaf982d613b41cd6c795c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CARBOXIMETILCELULOSA</topic><topic>CARBOXYMETHYLCELLULOSE</topic><topic>CITOLOGIA</topic><topic>CRYPTOCOCCUS (CHAMPIGNON)</topic><topic>CRYPTOCOCCUS (HONGO)</topic><topic>CYTOLOGIE</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GLICERALDEHIDO 3 FOSF DESHIDROG</topic><topic>GLICOSIDASAS</topic><topic>GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO</topic><topic>GLYCOSIDASE</topic><topic>Methods. Procedures. Technologies</topic><topic>Modification of gene expression level</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>REPONSE IMMUNITAIRE</topic><topic>RESPUESTA INMUNOLOGICA</topic><topic>SACCHAROMYCES CEREVISIAE</topic><toplevel>online_resources</toplevel><creatorcontrib>Hatano, Takushi</creatorcontrib><creatorcontrib>Komura, Tsunehiro</creatorcontrib><creatorcontrib>Cui, Zhifeng</creatorcontrib><creatorcontrib>Mochizuki, Daisuke</creatorcontrib><creatorcontrib>Miyakawa, Tokichi</creatorcontrib><creatorcontrib>Matsuzaki, Hiroaki</creatorcontrib><creatorcontrib>Fukui, Sakuzo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of fermentation and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hatano, Takushi</au><au>Komura, Tsunehiro</au><au>Cui, Zhifeng</au><au>Mochizuki, Daisuke</au><au>Miyakawa, Tokichi</au><au>Matsuzaki, Hiroaki</au><au>Fukui, Sakuzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae</atitle><jtitle>Journal of fermentation and bioengineering</jtitle><date>1994-01-01</date><risdate>1994</risdate><volume>77</volume><issue>3</issue><spage>235</spage><epage>238</epage><pages>235-238</pages><issn>0922-338X</issn><coden>JFBIEX</coden><abstract>A cDNA copy of the carboxymethylcellulase (CMCase 1) gene,
CMC1, of the yeast
Cryptococcus flavus was expressed in
Saccharomyces cerevisiae under the control of the glyceraldehyde-3-phosphate dehydrogenase gene (
GAP) promoter derived from
S. cerevisiae. In glucose medium, extracellular production of a large amount of CMCase 1 was achieved by
S. cerevisiae cells transformed with YEp-plasmid carrying
CMC1. Two major proteins (36 kDa and 34 kDa) secreted in the medium both had CMCase activity and immuno-responsibility to anti-CMCase 1-antibody. From an analysis of amino acid sequences, it was found that the 36 kDa protein is a pro-CMCase 1 having an N-terminal residue, Ala, the 19th amino acid of the peptide deduced from the nucleotide sequence of
CMC1 on the cDNA, and that the 34 kDa protein is a mature CMCase 1. Alteration of the cell morphology of
S. cerevisiae was induced by transformation with YEp-plasmid carrying
CMC1.</abstract><cop>Osaka</cop><pub>Elsevier B.V</pub><doi>10.1016/0922-338X(94)90225-9</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0922-338X |
| ispartof | Journal of fermentation and bioengineering, 1994-01, Vol.77 (3), p.235-238 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_16583307 |
| source | Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biotechnology CARBOXIMETILCELULOSA CARBOXYMETHYLCELLULOSE CITOLOGIA CRYPTOCOCCUS (CHAMPIGNON) CRYPTOCOCCUS (HONGO) CYTOLOGIE EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics GLICERALDEHIDO 3 FOSF DESHIDROG GLICOSIDASAS GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO GLYCOSIDASE Methods. Procedures. Technologies Modification of gene expression level PLASMIDE PLASMIDIOS REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA SACCHAROMYCES CEREVISIAE |
| title | Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae |
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