Detection of Tumor-derived DNA in Cerebrospinal Fluid

The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient...

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Veröffentlicht in:Journal of neuropathology and experimental neurology 1994-07, Vol.53 (4), p.364-368
Hauptverfasser: Rhodes, C Harker, Honsinger, Charles, Sorenson, George D
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container_title Journal of neuropathology and experimental neurology
container_volume 53
creator Rhodes, C Harker
Honsinger, Charles
Sorenson, George D
description The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. This report demonstrates that CSF sometimes contains tumor-derived DNA and suggests that PCR examination of CSF DNA may be diagnostically useful.
doi_str_mv 10.1097/00005072-199407000-00007
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We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. 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Intracranial pressure determination</subject><subject>DNA, Neoplasm - cerebrospinal fluid</subject><subject>ErbB Receptors - genetics</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Glioblastoma - cerebrospinal fluid</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Receptor, ErbB-2</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kVFPwyAQx4nRzDn9CCZ9ML5VoaWlPC6bU5NFX-YzoXBkKGsntC5-e5mre5OXC__73cH9D6GE4DuCObvH8RSYZSnhnGIWb-leYidoTIqCpmXBqlM0xjjL0hyX_BxdhPAeCY45HaER4yRjGR2jYg4dqM62TdKaZNVvWp9q8PYLdDJ_mSa2SWbgofZt2NpGumTheqsv0ZmRLsDVECfobfGwmj2ly9fH59l0maq8oiw1sqK80hJUmWekIMzUmCpuDJGFkowwYmjJsay5hijkDEtdGlYCMFwz0PkE3R76bn372UPoxMYGBc7JBto-CFIWVRwkj2B1AFX8aPBgxNbbjfTfgmCxd0z8OSaOjv1KLJZeD2_09Qb0sXCwKOZvhrwMSjrjZaNsOGI5jSOUVcToAdu1rgMfPly_Ay_WIF23Fv_tK_8BOsuBig</recordid><startdate>199407</startdate><enddate>199407</enddate><creator>Rhodes, C Harker</creator><creator>Honsinger, Charles</creator><creator>Sorenson, George D</creator><general>American Association of Neuropathologists, Inc</general><general>Lippincott Williams &amp; Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>199407</creationdate><title>Detection of Tumor-derived DNA in Cerebrospinal Fluid</title><author>Rhodes, C Harker ; Honsinger, Charles ; Sorenson, George D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3847-fa8498daec6321517fb04c9ff1a5ca7171f4690ab9de5ca370ad6f76ee70b7ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Brain Neoplasms - cerebrospinal fluid</topic><topic>Brain Neoplasms - secondary</topic><topic>Breast Neoplasms - cerebrospinal fluid</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinoma, Ductal, Breast - cerebrospinal fluid</topic><topic>Carcinoma, Ductal, Breast - secondary</topic><topic>Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). 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source MEDLINE; Oxford University Press Archive; Journals@Ovid Complete
subjects Aged
Aged, 80 and over
Biological and medical sciences
Brain Neoplasms - cerebrospinal fluid
Brain Neoplasms - secondary
Breast Neoplasms - cerebrospinal fluid
Breast Neoplasms - pathology
Carcinoma, Ductal, Breast - cerebrospinal fluid
Carcinoma, Ductal, Breast - secondary
Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination
DNA, Neoplasm - cerebrospinal fluid
ErbB Receptors - genetics
Female
Gene Amplification
Glioblastoma - cerebrospinal fluid
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Proto-Oncogene Proteins - genetics
Receptor, ErbB-2
title Detection of Tumor-derived DNA in Cerebrospinal Fluid
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