Detection of Tumor-derived DNA in Cerebrospinal Fluid
The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient...
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| Veröffentlicht in: | Journal of neuropathology and experimental neurology 1994-07, Vol.53 (4), p.364-368 |
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| container_end_page | 368 |
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| container_issue | 4 |
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| container_title | Journal of neuropathology and experimental neurology |
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| creator | Rhodes, C Harker Honsinger, Charles Sorenson, George D |
| description | The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. This report demonstrates that CSF sometimes contains tumor-derived DNA and suggests that PCR examination of CSF DNA may be diagnostically useful. |
| doi_str_mv | 10.1097/00005072-199407000-00007 |
| format | Article |
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We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. 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Intracranial pressure determination ; DNA, Neoplasm - cerebrospinal fluid ; ErbB Receptors - genetics ; Female ; Gene Amplification ; Glioblastoma - cerebrospinal fluid ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Proto-Oncogene Proteins - genetics ; Receptor, ErbB-2</subject><ispartof>Journal of neuropathology and experimental neurology, 1994-07, Vol.53 (4), p.364-368</ispartof><rights>1994 American Association of Neuropathologists, Inc</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3847-fa8498daec6321517fb04c9ff1a5ca7171f4690ab9de5ca370ad6f76ee70b7ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3446968$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7912724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rhodes, C Harker</creatorcontrib><creatorcontrib>Honsinger, Charles</creatorcontrib><creatorcontrib>Sorenson, George D</creatorcontrib><title>Detection of Tumor-derived DNA in Cerebrospinal Fluid</title><title>Journal of neuropathology and experimental neurology</title><addtitle>J Neuropathol Exp Neurol</addtitle><description>The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. This report demonstrates that CSF sometimes contains tumor-derived DNA and suggests that PCR examination of CSF DNA may be diagnostically useful.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Brain Neoplasms - cerebrospinal fluid</subject><subject>Brain Neoplasms - secondary</subject><subject>Breast Neoplasms - cerebrospinal fluid</subject><subject>Breast Neoplasms - pathology</subject><subject>Carcinoma, Ductal, Breast - cerebrospinal fluid</subject><subject>Carcinoma, Ductal, Breast - secondary</subject><subject>Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination</subject><subject>DNA, Neoplasm - cerebrospinal fluid</subject><subject>ErbB Receptors - genetics</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Glioblastoma - cerebrospinal fluid</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Receptor, ErbB-2</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kVFPwyAQx4nRzDn9CCZ9ML5VoaWlPC6bU5NFX-YzoXBkKGsntC5-e5mre5OXC__73cH9D6GE4DuCObvH8RSYZSnhnGIWb-leYidoTIqCpmXBqlM0xjjL0hyX_BxdhPAeCY45HaER4yRjGR2jYg4dqM62TdKaZNVvWp9q8PYLdDJ_mSa2SWbgofZt2NpGumTheqsv0ZmRLsDVECfobfGwmj2ly9fH59l0maq8oiw1sqK80hJUmWekIMzUmCpuDJGFkowwYmjJsay5hijkDEtdGlYCMFwz0PkE3R76bn372UPoxMYGBc7JBto-CFIWVRwkj2B1AFX8aPBgxNbbjfTfgmCxd0z8OSaOjv1KLJZeD2_09Qb0sXCwKOZvhrwMSjrjZaNsOGI5jSOUVcToAdu1rgMfPly_Ay_WIF23Fv_tK_8BOsuBig</recordid><startdate>199407</startdate><enddate>199407</enddate><creator>Rhodes, C Harker</creator><creator>Honsinger, Charles</creator><creator>Sorenson, George D</creator><general>American Association of Neuropathologists, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>199407</creationdate><title>Detection of Tumor-derived DNA in Cerebrospinal Fluid</title><author>Rhodes, C Harker ; Honsinger, Charles ; Sorenson, George D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3847-fa8498daec6321517fb04c9ff1a5ca7171f4690ab9de5ca370ad6f76ee70b7ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Brain Neoplasms - cerebrospinal fluid</topic><topic>Brain Neoplasms - secondary</topic><topic>Breast Neoplasms - cerebrospinal fluid</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinoma, Ductal, Breast - cerebrospinal fluid</topic><topic>Carcinoma, Ductal, Breast - secondary</topic><topic>Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination</topic><topic>DNA, Neoplasm - cerebrospinal fluid</topic><topic>ErbB Receptors - genetics</topic><topic>Female</topic><topic>Gene Amplification</topic><topic>Glioblastoma - cerebrospinal fluid</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Receptor, ErbB-2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rhodes, C Harker</creatorcontrib><creatorcontrib>Honsinger, Charles</creatorcontrib><creatorcontrib>Sorenson, George D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuropathology and experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rhodes, C Harker</au><au>Honsinger, Charles</au><au>Sorenson, George D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Tumor-derived DNA in Cerebrospinal Fluid</atitle><jtitle>Journal of neuropathology and experimental neurology</jtitle><addtitle>J Neuropathol Exp Neurol</addtitle><date>1994-07</date><risdate>1994</risdate><volume>53</volume><issue>4</issue><spage>364</spage><epage>368</epage><pages>364-368</pages><issn>0022-3069</issn><eissn>1554-6578</eissn><coden>JNENAD</coden><abstract>The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2/neu gene sequences were present in her CSF. The other patient had a glioblastoma which contained amplified epidermal growth factor receptor (EGFR) genes, and amplified EGFR gene sequences were present in her CSF. This report demonstrates that CSF sometimes contains tumor-derived DNA and suggests that PCR examination of CSF DNA may be diagnostically useful.</abstract><cop>Hagerstown, MD</cop><pub>American Association of Neuropathologists, Inc</pub><pmid>7912724</pmid><doi>10.1097/00005072-199407000-00007</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of neuropathology and experimental neurology, 1994-07, Vol.53 (4), p.364-368 |
| issn | 0022-3069 1554-6578 |
| language | eng |
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| source | MEDLINE; Oxford University Press Archive; Journals@Ovid Complete |
| subjects | Aged Aged, 80 and over Biological and medical sciences Brain Neoplasms - cerebrospinal fluid Brain Neoplasms - secondary Breast Neoplasms - cerebrospinal fluid Breast Neoplasms - pathology Carcinoma, Ductal, Breast - cerebrospinal fluid Carcinoma, Ductal, Breast - secondary Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination DNA, Neoplasm - cerebrospinal fluid ErbB Receptors - genetics Female Gene Amplification Glioblastoma - cerebrospinal fluid Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Proto-Oncogene Proteins - genetics Receptor, ErbB-2 |
| title | Detection of Tumor-derived DNA in Cerebrospinal Fluid |
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