Conditional regulatory elements of human immunodeficiency virus type 2 long terminal repeat
Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, U.S.A. Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the...
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| Veröffentlicht in: | Journal of general virology 1994-09, Vol.75 (9), p.2253-2260 |
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| container_title | Journal of general virology |
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| creator | Arya, Suresh K Mohr, Jennifer R |
| description | Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, U.S.A.
Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the U3 region, located upstream of the enhancer-promoter region. These elements acted in a cell type-specific manner, being most active in human lymphocytic CEM cells, more active in Jurkat cells than in human monocytic U937 cells and least active in epithelioid HeLa cells. The down-modulatory effect of the negative regulatory element was abolished by HIV-2 Tat, suggesting the involvement of upstream DNA elements in optimal Tat-mediated trans-activation. The sequence elements that respond to T cell activation signals were also located in the upstream U3 region. Notably, the magnitude of the effect of the upstream regulatory elements depended on the basal activity of the LTR, which was also cell type-dependent. This emphasizes the importance of the cell-specific transcriptional factors and other effectors in regulating HIV gene expression. These observations may be relevant to the cell type-specific restriction of virus replication in vivo .
Received 7 March 1994;
accepted 15 April 1994. |
| doi_str_mv | 10.1099/0022-1317-75-9-2253 |
| format | Article |
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Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the U3 region, located upstream of the enhancer-promoter region. These elements acted in a cell type-specific manner, being most active in human lymphocytic CEM cells, more active in Jurkat cells than in human monocytic U937 cells and least active in epithelioid HeLa cells. The down-modulatory effect of the negative regulatory element was abolished by HIV-2 Tat, suggesting the involvement of upstream DNA elements in optimal Tat-mediated trans-activation. The sequence elements that respond to T cell activation signals were also located in the upstream U3 region. Notably, the magnitude of the effect of the upstream regulatory elements depended on the basal activity of the LTR, which was also cell type-dependent. This emphasizes the importance of the cell-specific transcriptional factors and other effectors in regulating HIV gene expression. These observations may be relevant to the cell type-specific restriction of virus replication in vivo .
Received 7 March 1994;
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Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the U3 region, located upstream of the enhancer-promoter region. These elements acted in a cell type-specific manner, being most active in human lymphocytic CEM cells, more active in Jurkat cells than in human monocytic U937 cells and least active in epithelioid HeLa cells. The down-modulatory effect of the negative regulatory element was abolished by HIV-2 Tat, suggesting the involvement of upstream DNA elements in optimal Tat-mediated trans-activation. The sequence elements that respond to T cell activation signals were also located in the upstream U3 region. Notably, the magnitude of the effect of the upstream regulatory elements depended on the basal activity of the LTR, which was also cell type-dependent. This emphasizes the importance of the cell-specific transcriptional factors and other effectors in regulating HIV gene expression. These observations may be relevant to the cell type-specific restriction of virus replication in vivo .
Received 7 March 1994;
accepted 15 April 1994.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>Cloning, Molecular</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetics</subject><subject>HeLa Cells</subject><subject>HIV Long Terminal Repeat</subject><subject>HIV-2 - genetics</subject><subject>human immunodeficiency virus 2</subject><subject>Humans</subject><subject>Lymphocytes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Promoter Regions, Genetic</subject><subject>Proviruses - genetics</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Transfection</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1rGzEQhkVoSN0kvyAUdCill231udo9FtOPQCCX9NSDmJVHtspKcqXdFv_7rrFxT3N4n3lneAh54OwjZ33_iTEhGi65aYxu-kYILa_IiqtWN2LJX5HVhXhN3tT6izGulDY35KZjxvRCrsjPdU6bMIWcYKQFt_MIUy4HiiNGTFOl2dPdHCHREOOc8gZ9cAGTO9A_ocyVToc9UkHHnLZ0whLDqWiPMN2Raw9jxfvzvCU_vn55WX9vnp6_Pa4_PzVOyW5qAAaPvBcD7wbGnOmYaqUHpRgOxnHATjiQg95A17veC3DQeiWd5tgOrWvlLXl_6t2X_HvGOtkYqsNxhIR5rpa32nSaiwWUJ9CVXGtBb_clRCgHy5k9KrVHYfYozBpte3tUumy9PdfPQ8TNZefscMnfnXOoDkZfILlQL5gSQpmWL9iHE7YL293fUNBuMcWwvDKEbBeZ_y_-A1PMjnY</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Arya, Suresh K</creator><creator>Mohr, Jennifer R</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19940901</creationdate><title>Conditional regulatory elements of human immunodeficiency virus type 2 long terminal repeat</title><author>Arya, Suresh K ; Mohr, Jennifer R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-aabfe192b18b00c780463fa440eb7c1ae82ca3b5da89c9f2aca6f43c51e6b6c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>Cloning, Molecular</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - genetics</topic><topic>Enhancer Elements, Genetic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetics</topic><topic>HeLa Cells</topic><topic>HIV Long Terminal Repeat</topic><topic>HIV-2 - genetics</topic><topic>human immunodeficiency virus 2</topic><topic>Humans</topic><topic>Lymphocytes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Promoter Regions, Genetic</topic><topic>Proviruses - genetics</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Transfection</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arya, Suresh K</creatorcontrib><creatorcontrib>Mohr, Jennifer R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arya, Suresh K</au><au>Mohr, Jennifer R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conditional regulatory elements of human immunodeficiency virus type 2 long terminal repeat</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>75</volume><issue>9</issue><spage>2253</spage><epage>2260</epage><pages>2253-2260</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, U.S.A.
Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) long terminal repeat (LTR) revealed a novel cis-acting positive and a negative regulatory element in the U3 region, located upstream of the enhancer-promoter region. These elements acted in a cell type-specific manner, being most active in human lymphocytic CEM cells, more active in Jurkat cells than in human monocytic U937 cells and least active in epithelioid HeLa cells. The down-modulatory effect of the negative regulatory element was abolished by HIV-2 Tat, suggesting the involvement of upstream DNA elements in optimal Tat-mediated trans-activation. The sequence elements that respond to T cell activation signals were also located in the upstream U3 region. Notably, the magnitude of the effect of the upstream regulatory elements depended on the basal activity of the LTR, which was also cell type-dependent. This emphasizes the importance of the cell-specific transcriptional factors and other effectors in regulating HIV gene expression. These observations may be relevant to the cell type-specific restriction of virus replication in vivo .
Received 7 March 1994;
accepted 15 April 1994.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8077923</pmid><doi>10.1099/0022-1317-75-9-2253</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1994-09, Vol.75 (9), p.2253-2260 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Cell Line Chloramphenicol O-Acetyltransferase - biosynthesis Cloning, Molecular DNA Mutational Analysis DNA, Viral - chemistry DNA, Viral - genetics Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene Expression Genetics HeLa Cells HIV Long Terminal Repeat HIV-2 - genetics human immunodeficiency virus 2 Humans Lymphocytes Microbiology Molecular Sequence Data Mutagenesis Promoter Regions, Genetic Proviruses - genetics Regulatory Sequences, Nucleic Acid Transfection Virology |
| title | Conditional regulatory elements of human immunodeficiency virus type 2 long terminal repeat |
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