Purification of α-amylase from Bacillus lentus cultures

Acetone fractionation of Bacillus lentus culture filtrate yielded the highest alpha -amylase activity and the 66.6% fraction reached 13-fold that of the crude enzyme preparation. Gel filtration and ion exchange chromatography afforded a pure alpha -amylase (relative molecular mass, 42 000). The pure...

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Veröffentlicht in:	Applied microbiology and biotechnology 1992-12, Vol.38 (3), p.312-314
Hauptverfasser:	EL-AASSAR, S. A, OMAR, S. H, GOUDA, M. K, ISMAIL, A. M, ABDEL-FATTAH, A. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus lentus Biological and medical sciences Biotechnology Enzyme engineering Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies
Online-Zugang:	Volltext
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