Functional characterization of flavin-containing monooxygenase 1B1 expressed in Saccharomyces cerevisiae and Escherichia coli and analysis of proposed FAD- and membrane-binding domains

A cDNA encoding the flavin-containing monooxygenase of rabbit lung (FMO 1B1) was expressed in yeast and Escherichia coli and the recombinant enzymes characterized. A high copy, isopropyl-1-thio-beta-D-galactopyranoside (IPTG)-inducible E. coli expression vector, pKKHC, was used for expression in E....

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Veröffentlicht in:	The Journal of biological chemistry 1993-03, Vol.268 (8), p.5728-5734
Hauptverfasser:	LAWTON, M. P, PHILPOT, R. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Base Sequence binding Binding Sites Biological and medical sciences Catalysis Cell Membrane - metabolism characterization Cloning, Molecular DNA Enzymes and enzyme inhibitors Escherichia coli FAD Flavin-Adenine Dinucleotide - metabolism flavin-containing monooxygenase Fundamental and applied biological sciences. Psychology lung Molecular Sequence Data Mutation Oxidoreductases Oxygenases - genetics Oxygenases - metabolism Rabbits Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Sequence Deletion sites
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