A Sensitive Approach to Map Genome-wide 5-Hydroxymethylcytosine and 5-Formylcytosine at Single-Base Resolution

Mapping genome-wide 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) at single-base resolution is important to understand their biological functions. We present a cost-efficient mapping method that combines 5hmC-specific restriction enzyme PvuRts1I with a 5hmC chemical labeling enrichment m...

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Veröffentlicht in:	Molecular cell 2015-02, Vol.57 (4), p.750-761
Hauptverfasser:	Sun, Zhiyi, Dai, Nan, Borgaro, Janine G., Quimby, Aine, Sun, Dapeng, Corrêa, Ivan R., Zheng, Yu, Zhu, Zhenyu, Guan, Shengxi
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methylcytosine - analogs & derivatives Animals Base Sequence Cytosine - analogs & derivatives Cytosine - chemistry DNA Restriction Enzymes - chemistry Embryonic Stem Cells Epigenesis, Genetic Gene Library Histones - metabolism Mice Restriction Mapping - methods
Online-Zugang:	Volltext
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