cold‐induced myo‐inositol transporter‐like gene confers tolerance to multiple abiotic stresses in transgenic tobacco plants
A full length cDNA encoding a myo‐inositol transporter‐like protein, named as MfINT‐like, was cloned from Medicago sativa subsp. falcata (herein falcata), a species with greater cold tolerance than alfalfa (M. sativa subsp. sativa). MfINT‐like is located on plasma membranes. MfINT‐like transcript wa...
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| Veröffentlicht in: | Physiologia plantarum 2015-03, Vol.153 (3), p.355-364 |
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| creator | Sambe, Mame Abdou Nahr He, Xueying Tu, Qinghua Guo, Zhenfei |
| description | A full length cDNA encoding a myo‐inositol transporter‐like protein, named as MfINT‐like, was cloned from Medicago sativa subsp. falcata (herein falcata), a species with greater cold tolerance than alfalfa (M. sativa subsp. sativa). MfINT‐like is located on plasma membranes. MfINT‐like transcript was induced 2–4 h after exogenous myo‐inositol treatment, 24–96 h with cold, and 96 h by salinity. Given that myo‐inositol accumulates higher in falcata after 24 h of cold treatment, myo‐inositol is proposed to be involved in cold‐induced expression of MfINT‐like. Higher levels of myo‐inositol was observed in leaves of transgenic tobacco plants overexpressing MfINT‐like than the wild‐type but not in the roots of plants grown on myo‐inositol containing medium, suggesting that transgenic plants had higher myo‐inositol transport activity than the wild‐type. Transgenic plants survived better to freezing temperature, and had lower ion leakage and higher maximal photochemical efficiency of photosystem II (Fᵥ/Fₘ) after chilling treatment. In addition, greater plant fresh weight was observed in transgenic plants as compared with the wild‐type when plants were grown under drought or salinity stress. The results suggest that MfINT‐like mediated transport of myo‐inositol is associated with plant tolerance to abiotic stresses. |
| doi_str_mv | 10.1111/ppl.12249 |
| format | Article |
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MfINT‐like is located on plasma membranes. MfINT‐like transcript was induced 2–4 h after exogenous myo‐inositol treatment, 24–96 h with cold, and 96 h by salinity. Given that myo‐inositol accumulates higher in falcata after 24 h of cold treatment, myo‐inositol is proposed to be involved in cold‐induced expression of MfINT‐like. Higher levels of myo‐inositol was observed in leaves of transgenic tobacco plants overexpressing MfINT‐like than the wild‐type but not in the roots of plants grown on myo‐inositol containing medium, suggesting that transgenic plants had higher myo‐inositol transport activity than the wild‐type. Transgenic plants survived better to freezing temperature, and had lower ion leakage and higher maximal photochemical efficiency of photosystem II (Fᵥ/Fₘ) after chilling treatment. In addition, greater plant fresh weight was observed in transgenic plants as compared with the wild‐type when plants were grown under drought or salinity stress. The results suggest that MfINT‐like mediated transport of myo‐inositol is associated with plant tolerance to abiotic stresses.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.12249</identifier><identifier>PMID: 25131886</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Abiotic stress ; Acclimatization ; alfalfa ; Base Sequence ; Cold ; Cold Temperature ; cold tolerance ; cold treatment ; complementary DNA ; DNA, Complementary - genetics ; drought ; Droughts ; freezing point ; Gene Expression Regulation, Plant ; gene overexpression ; genes ; Inositol - metabolism ; Inositol - pharmacology ; leaves ; Medicago sativa - genetics ; Medicago sativa subsp. falcata ; Molecular Sequence Data ; myo-inositol ; Nicotiana - drug effects ; Nicotiana - genetics ; Nicotiana - physiology ; photosystem II ; Plant Leaves - drug effects ; Plant Leaves - genetics ; Plant Leaves - physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; plasma membrane ; roots ; Salinity ; salt stress ; Sequence Analysis, DNA ; Stress, Physiological ; Transgenic plants</subject><ispartof>Physiologia plantarum, 2015-03, Vol.153 (3), p.355-364</ispartof><rights>2014 Scandinavian Plant Physiology Society</rights><rights>2014 Scandinavian Plant Physiology Society.</rights><rights>2015 Scandinavian Plant Physiology Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4859-bb6c79a764ab2577f5ff01ce37e7916c8c5550d06952ecaef5dda2edc0d08ebc3</citedby><cites>FETCH-LOGICAL-c4859-bb6c79a764ab2577f5ff01ce37e7916c8c5550d06952ecaef5dda2edc0d08ebc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppl.12249$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppl.12249$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25131886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sambe, Mame Abdou Nahr</creatorcontrib><creatorcontrib>He, Xueying</creatorcontrib><creatorcontrib>Tu, Qinghua</creatorcontrib><creatorcontrib>Guo, Zhenfei</creatorcontrib><title>cold‐induced myo‐inositol transporter‐like gene confers tolerance to multiple abiotic stresses in transgenic tobacco plants</title><title>Physiologia plantarum</title><addtitle>Physiol Plantarum</addtitle><description>A full length cDNA encoding a myo‐inositol transporter‐like protein, named as MfINT‐like, was cloned from Medicago sativa subsp. falcata (herein falcata), a species with greater cold tolerance than alfalfa (M. sativa subsp. sativa). MfINT‐like is located on plasma membranes. MfINT‐like transcript was induced 2–4 h after exogenous myo‐inositol treatment, 24–96 h with cold, and 96 h by salinity. Given that myo‐inositol accumulates higher in falcata after 24 h of cold treatment, myo‐inositol is proposed to be involved in cold‐induced expression of MfINT‐like. Higher levels of myo‐inositol was observed in leaves of transgenic tobacco plants overexpressing MfINT‐like than the wild‐type but not in the roots of plants grown on myo‐inositol containing medium, suggesting that transgenic plants had higher myo‐inositol transport activity than the wild‐type. Transgenic plants survived better to freezing temperature, and had lower ion leakage and higher maximal photochemical efficiency of photosystem II (Fᵥ/Fₘ) after chilling treatment. In addition, greater plant fresh weight was observed in transgenic plants as compared with the wild‐type when plants were grown under drought or salinity stress. The results suggest that MfINT‐like mediated transport of myo‐inositol is associated with plant tolerance to abiotic stresses.</description><subject>Abiotic stress</subject><subject>Acclimatization</subject><subject>alfalfa</subject><subject>Base Sequence</subject><subject>Cold</subject><subject>Cold Temperature</subject><subject>cold tolerance</subject><subject>cold treatment</subject><subject>complementary DNA</subject><subject>DNA, Complementary - genetics</subject><subject>drought</subject><subject>Droughts</subject><subject>freezing point</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene overexpression</subject><subject>genes</subject><subject>Inositol - metabolism</subject><subject>Inositol - pharmacology</subject><subject>leaves</subject><subject>Medicago sativa - genetics</subject><subject>Medicago sativa subsp. falcata</subject><subject>Molecular Sequence Data</subject><subject>myo-inositol</subject><subject>Nicotiana - drug effects</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>photosystem II</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>plasma membrane</subject><subject>roots</subject><subject>Salinity</subject><subject>salt stress</subject><subject>Sequence Analysis, DNA</subject><subject>Stress, Physiological</subject><subject>Transgenic plants</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1ks1u1DAUhS0EokNhwQuAJTawSOuf2I6XqKIFaQQVQ8vScpybym0Sp3YiOjt4A56RJ8HTtF0g4Y19r75zdKRjhF5SckDzORzH7oAyVupHaEW51gUnonyMVoRwWmhO1R56ltIlIVRKyp6iPSYop1UlV-iXC13z5-dvPzSzgwb323A7heSn0OEp2iGNIU4Q87rzV4AvYADswtBCTDgzkBEH-YX7uZv82AG2tQ-TdzhNEVKChP2wOGVtXk-hts4FPHZ2mNJz9KS1XYIXd_c-Ojv-8O3oY7H-cvLp6P26cGUldFHX0iltlSxtzYRSrWhbQh1wBUpT6SonhCANkVowcBZa0TSWQePyroLa8X30dvEdY7ieIU2m98lBl0NAmJOhUijOGBcso2_-QS_DHIecbkcJXUpdqUy9WygXQ0oRWjNG39u4NZSYXS8m92Jue8nsqzvHue6heSDvi8jA4QL88B1s_-9kTk_X95bFovBpgpsHhY1XRiquhPn--cTITSW-nm_OzS7u64VvbTD2IvpkzjaMUJG_RaVKJflfio214A</recordid><startdate>201503</startdate><enddate>201503</enddate><creator>Sambe, Mame Abdou Nahr</creator><creator>He, Xueying</creator><creator>Tu, Qinghua</creator><creator>Guo, Zhenfei</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201503</creationdate><title>cold‐induced myo‐inositol transporter‐like gene confers tolerance to multiple abiotic stresses in transgenic tobacco plants</title><author>Sambe, Mame Abdou Nahr ; He, Xueying ; Tu, Qinghua ; Guo, Zhenfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4859-bb6c79a764ab2577f5ff01ce37e7916c8c5550d06952ecaef5dda2edc0d08ebc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abiotic stress</topic><topic>Acclimatization</topic><topic>alfalfa</topic><topic>Base Sequence</topic><topic>Cold</topic><topic>Cold Temperature</topic><topic>cold tolerance</topic><topic>cold treatment</topic><topic>complementary DNA</topic><topic>DNA, Complementary - genetics</topic><topic>drought</topic><topic>Droughts</topic><topic>freezing point</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene overexpression</topic><topic>genes</topic><topic>Inositol - metabolism</topic><topic>Inositol - pharmacology</topic><topic>leaves</topic><topic>Medicago sativa - genetics</topic><topic>Medicago sativa subsp. falcata</topic><topic>Molecular Sequence Data</topic><topic>myo-inositol</topic><topic>Nicotiana - drug effects</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>photosystem II</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - physiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>plasma membrane</topic><topic>roots</topic><topic>Salinity</topic><topic>salt stress</topic><topic>Sequence Analysis, DNA</topic><topic>Stress, Physiological</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sambe, Mame Abdou Nahr</creatorcontrib><creatorcontrib>He, Xueying</creatorcontrib><creatorcontrib>Tu, Qinghua</creatorcontrib><creatorcontrib>Guo, Zhenfei</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sambe, Mame Abdou Nahr</au><au>He, Xueying</au><au>Tu, Qinghua</au><au>Guo, Zhenfei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cold‐induced myo‐inositol transporter‐like gene confers tolerance to multiple abiotic stresses in transgenic tobacco plants</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plantarum</addtitle><date>2015-03</date><risdate>2015</risdate><volume>153</volume><issue>3</issue><spage>355</spage><epage>364</epage><pages>355-364</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><abstract>A full length cDNA encoding a myo‐inositol transporter‐like protein, named as MfINT‐like, was cloned from Medicago sativa subsp. falcata (herein falcata), a species with greater cold tolerance than alfalfa (M. sativa subsp. sativa). MfINT‐like is located on plasma membranes. MfINT‐like transcript was induced 2–4 h after exogenous myo‐inositol treatment, 24–96 h with cold, and 96 h by salinity. Given that myo‐inositol accumulates higher in falcata after 24 h of cold treatment, myo‐inositol is proposed to be involved in cold‐induced expression of MfINT‐like. Higher levels of myo‐inositol was observed in leaves of transgenic tobacco plants overexpressing MfINT‐like than the wild‐type but not in the roots of plants grown on myo‐inositol containing medium, suggesting that transgenic plants had higher myo‐inositol transport activity than the wild‐type. Transgenic plants survived better to freezing temperature, and had lower ion leakage and higher maximal photochemical efficiency of photosystem II (Fᵥ/Fₘ) after chilling treatment. In addition, greater plant fresh weight was observed in transgenic plants as compared with the wild‐type when plants were grown under drought or salinity stress. The results suggest that MfINT‐like mediated transport of myo‐inositol is associated with plant tolerance to abiotic stresses.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>25131886</pmid><doi>10.1111/ppl.12249</doi><tpages>10</tpages></addata></record> |
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| subjects | Abiotic stress Acclimatization alfalfa Base Sequence Cold Cold Temperature cold tolerance cold treatment complementary DNA DNA, Complementary - genetics drought Droughts freezing point Gene Expression Regulation, Plant gene overexpression genes Inositol - metabolism Inositol - pharmacology leaves Medicago sativa - genetics Medicago sativa subsp. falcata Molecular Sequence Data myo-inositol Nicotiana - drug effects Nicotiana - genetics Nicotiana - physiology photosystem II Plant Leaves - drug effects Plant Leaves - genetics Plant Leaves - physiology Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified plasma membrane roots Salinity salt stress Sequence Analysis, DNA Stress, Physiological Transgenic plants |
| title | cold‐induced myo‐inositol transporter‐like gene confers tolerance to multiple abiotic stresses in transgenic tobacco plants |
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