Evaluation of novel second-generation RSV and influenza rapid tests at the point of care
Abstract Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Adminis...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2015-03, Vol.81 (3), p.171-176 |
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| creator | Tuttle, Ryan Weick, Anja Schwarz, Wiebke Sabrina Chen, Xi Obermeier, Patrick Seeber, Lea Tief, Franziska Muehlhans, Susann Karsch, Katharina Peiser, Christian Duwe, Susanne Schweiger, Brunhilde Rath, Barbara |
| description | Abstract Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA™-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA™-RSV rapid test. In the Charité Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA™-RSV and SOFIA™-Influenza A + B. Compared to real-time PCR, SOFIA™-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA™-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2 years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2 days of illness and with nasopharyngeal compared to nasal swabs ( P = 0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management. |
| doi_str_mv | 10.1016/j.diagmicrobio.2014.11.013 |
| format | Article |
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Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA™-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA™-RSV rapid test. In the Charité Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA™-RSV and SOFIA™-Influenza A + B. Compared to real-time PCR, SOFIA™-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA™-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2 years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2 days of illness and with nasopharyngeal compared to nasal swabs ( P = 0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2014.11.013</identifier><identifier>PMID: 25583129</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Child ; Child, Preschool ; Diagnostic Tests, Routine - methods ; Female ; Humans ; Infant ; Infant, Newborn ; Infectious Disease ; Influenza A virus - isolation & purification ; Influenza B virus - isolation & purification ; Influenza, Human - diagnosis ; Influenza-like illness ; Internal Medicine ; Male ; Minimally-invasive testing ; Nasopharynx - virology ; Point-of-care diagnostics ; Point-of-Care Systems ; Prospective Studies ; Rapid testing ; Respiratory syncytial virus ; Respiratory Syncytial Virus Infections - diagnosis ; Respiratory Syncytial Viruses - isolation & purification ; Sensitivity and Specificity ; Time Factors</subject><ispartof>Diagnostic microbiology and infectious disease, 2015-03, Vol.81 (3), p.171-176</ispartof><rights>Elsevier Inc.</rights><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-6f3209641ab3be8ddf4b52ea9857818be7e35b383447a545a3d773cc42f52eb93</citedby><cites>FETCH-LOGICAL-c505t-6f3209641ab3be8ddf4b52ea9857818be7e35b383447a545a3d773cc42f52eb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889314004842$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25583129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tuttle, Ryan</creatorcontrib><creatorcontrib>Weick, Anja</creatorcontrib><creatorcontrib>Schwarz, Wiebke Sabrina</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Obermeier, Patrick</creatorcontrib><creatorcontrib>Seeber, Lea</creatorcontrib><creatorcontrib>Tief, Franziska</creatorcontrib><creatorcontrib>Muehlhans, Susann</creatorcontrib><creatorcontrib>Karsch, Katharina</creatorcontrib><creatorcontrib>Peiser, Christian</creatorcontrib><creatorcontrib>Duwe, Susanne</creatorcontrib><creatorcontrib>Schweiger, Brunhilde</creatorcontrib><creatorcontrib>Rath, Barbara</creatorcontrib><title>Evaluation of novel second-generation RSV and influenza rapid tests at the point of care</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA™-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA™-RSV rapid test. In the Charité Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA™-RSV and SOFIA™-Influenza A + B. Compared to real-time PCR, SOFIA™-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA™-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2 years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2 days of illness and with nasopharyngeal compared to nasal swabs ( P = 0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management.</description><subject>Adolescent</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diagnostic Tests, Routine - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious Disease</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza B virus - isolation & purification</subject><subject>Influenza, Human - diagnosis</subject><subject>Influenza-like illness</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Minimally-invasive testing</subject><subject>Nasopharynx - virology</subject><subject>Point-of-care diagnostics</subject><subject>Point-of-Care Systems</subject><subject>Prospective Studies</subject><subject>Rapid testing</subject><subject>Respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus Infections - diagnosis</subject><subject>Respiratory Syncytial Viruses - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhoMo7rj6FyR48tJjKh_TaQ-CrOuusCC4Kt5COqleM_Yks0n3wPrrTTOriCdPIeSptypPEfIC2BoYbF5t1z7Ym11wOfUhrTkDuQZYMxAPyAp02zWMtewhWbFW8EbrTpyQJ6VsGQPeSfaYnHCltKiXFfl2frDjbKeQIk0DjemAIy3oUvTNDUbMx6dP11-pjZ6GOIwzxp-WZrsPnk5YpkLtRKfvSPcpxGlJcTbjU_JosGPBZ_fnKfny_vzz2WVz9fHiw9nbq8YppqZmMwjOuo0E24setfeD7BVH22nVatA9tihUL7SQsrVKKit82wrnJB8q1nfilLw85u5zup3rOGYXisNxtBHTXAxsVLUAHBb09RGt4krJOJh9Djub7wwws5g1W_O3WbOYNQCmmq3Fz-_7zP0O_Z_S3yor8O4IYP3tIWA2xQWMDn3I6CbjU_i_Pm_-iXFjiMHZ8QfeYdmmOcfq04Ap3DBzvex4WTFIxqSWXPwCh4ilgw</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Tuttle, Ryan</creator><creator>Weick, Anja</creator><creator>Schwarz, Wiebke Sabrina</creator><creator>Chen, Xi</creator><creator>Obermeier, Patrick</creator><creator>Seeber, Lea</creator><creator>Tief, Franziska</creator><creator>Muehlhans, Susann</creator><creator>Karsch, Katharina</creator><creator>Peiser, Christian</creator><creator>Duwe, Susanne</creator><creator>Schweiger, Brunhilde</creator><creator>Rath, Barbara</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150301</creationdate><title>Evaluation of novel second-generation RSV and influenza rapid tests at the point of care</title><author>Tuttle, Ryan ; Weick, Anja ; Schwarz, Wiebke Sabrina ; Chen, Xi ; Obermeier, Patrick ; Seeber, Lea ; Tief, Franziska ; Muehlhans, Susann ; Karsch, Katharina ; Peiser, Christian ; Duwe, Susanne ; Schweiger, Brunhilde ; Rath, Barbara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-6f3209641ab3be8ddf4b52ea9857818be7e35b383447a545a3d773cc42f52eb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Diagnostic Tests, Routine - methods</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious Disease</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza B virus - isolation & purification</topic><topic>Influenza, Human - diagnosis</topic><topic>Influenza-like illness</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Minimally-invasive testing</topic><topic>Nasopharynx - virology</topic><topic>Point-of-care diagnostics</topic><topic>Point-of-Care Systems</topic><topic>Prospective Studies</topic><topic>Rapid testing</topic><topic>Respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus Infections - diagnosis</topic><topic>Respiratory Syncytial Viruses - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tuttle, Ryan</creatorcontrib><creatorcontrib>Weick, Anja</creatorcontrib><creatorcontrib>Schwarz, Wiebke Sabrina</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Obermeier, Patrick</creatorcontrib><creatorcontrib>Seeber, Lea</creatorcontrib><creatorcontrib>Tief, Franziska</creatorcontrib><creatorcontrib>Muehlhans, Susann</creatorcontrib><creatorcontrib>Karsch, Katharina</creatorcontrib><creatorcontrib>Peiser, Christian</creatorcontrib><creatorcontrib>Duwe, Susanne</creatorcontrib><creatorcontrib>Schweiger, Brunhilde</creatorcontrib><creatorcontrib>Rath, Barbara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tuttle, Ryan</au><au>Weick, Anja</au><au>Schwarz, Wiebke Sabrina</au><au>Chen, Xi</au><au>Obermeier, Patrick</au><au>Seeber, Lea</au><au>Tief, Franziska</au><au>Muehlhans, Susann</au><au>Karsch, Katharina</au><au>Peiser, Christian</au><au>Duwe, Susanne</au><au>Schweiger, Brunhilde</au><au>Rath, Barbara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of novel second-generation RSV and influenza rapid tests at the point of care</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>81</volume><issue>3</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA™-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA™-RSV rapid test. In the Charité Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA™-RSV and SOFIA™-Influenza A + B. Compared to real-time PCR, SOFIA™-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA™-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2 years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2 days of illness and with nasopharyngeal compared to nasal swabs ( P = 0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25583129</pmid><doi>10.1016/j.diagmicrobio.2014.11.013</doi><tpages>6</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2015-03, Vol.81 (3), p.171-176 |
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| subjects | Adolescent Child Child, Preschool Diagnostic Tests, Routine - methods Female Humans Infant Infant, Newborn Infectious Disease Influenza A virus - isolation & purification Influenza B virus - isolation & purification Influenza, Human - diagnosis Influenza-like illness Internal Medicine Male Minimally-invasive testing Nasopharynx - virology Point-of-care diagnostics Point-of-Care Systems Prospective Studies Rapid testing Respiratory syncytial virus Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Viruses - isolation & purification Sensitivity and Specificity Time Factors |
| title | Evaluation of novel second-generation RSV and influenza rapid tests at the point of care |
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