Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria
Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria r...
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| Veröffentlicht in: | Journal of infection in developing countries 2015-02, Vol.9 (2), p.165-174 |
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| container_title | Journal of infection in developing countries |
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| creator | Ifeanyi, Casmir Ifeanyichukwu Cajetan Ikeneche, Nkiruka Florence Bassey, Bassey Enya Al-Gallas, Nazek Ben Aissa, Ridha Boudabous, Abdellatif |
| description | Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown.
Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied. E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.
Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.
This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria. |
| doi_str_mv | 10.3855/jidc.5528 |
| format | Article |
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Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied. E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.
Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.
This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.5528</identifier><identifier>PMID: 25699491</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Bacterial Adhesion ; Child, Preschool ; Diarrhea - microbiology ; E coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli - physiology ; Escherichia coli Infections - microbiology ; Feces - microbiology ; Female ; Genotype ; Hepatocytes - microbiology ; Humans ; Infant ; Infant, Newborn ; Male ; Nigeria ; Phenotype ; Polymerase Chain Reaction ; Virulence ; Virulence Factors - analysis ; Virulence Factors - genetics</subject><ispartof>Journal of infection in developing countries, 2015-02, Vol.9 (2), p.165-174</ispartof><rights>2015. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c228t-abbe4e45d9c4f45ffec6d5b552c3ee45672c27f0b863d24b33352fd0ca90659d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25699491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ifeanyi, Casmir Ifeanyichukwu Cajetan</creatorcontrib><creatorcontrib>Ikeneche, Nkiruka Florence</creatorcontrib><creatorcontrib>Bassey, Bassey Enya</creatorcontrib><creatorcontrib>Al-Gallas, Nazek</creatorcontrib><creatorcontrib>Ben Aissa, Ridha</creatorcontrib><creatorcontrib>Boudabous, Abdellatif</creatorcontrib><title>Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown.
Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied. E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.
Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.
This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.</description><subject>Bacterial Adhesion</subject><subject>Child, Preschool</subject><subject>Diarrhea - microbiology</subject><subject>E coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - physiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Genotype</subject><subject>Hepatocytes - microbiology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Nigeria</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Virulence</subject><subject>Virulence Factors - analysis</subject><subject>Virulence Factors - genetics</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkUtLAzEUhYMovhf-AQm4UbCax2Q6WZZaHyC60fWQSe50UqaTMckg3fjbTWkVcXUul49zHwehM0pueCHE7cIafSMEK3bQIZVjNmJ5QXb_1AfoKIQFIUJyQffRARO5lJmkh-jrzirvG1Bz6KzGs6Ab8FY3VmHtWot7FRsXVz0EbINrVQSDa--WOCGt8dDhTxsbbLYu2HY4NoDvwYBXLZ6q3sakb-C9jc6v8KQaFuoav9h5mqNO0F6t2gCnWz1G7_ezt-nj6Pn14Wk6eR5pxoo4UlUFGWTCSJ3Vmahr0LkRVTpZc0j9fMw0G9ekKnJuWFZxzgWrDdFKklxIw4_R5ca39-5jgBDLpQ0a2lZ14IZQ0lyMOSOUkIRe_EMXbvBd2q5MbyOU0YzKRF1tKO1dCB7qsvd2qfyqpKRch1KuQynXoST2fOs4VEswv-RPCvwb1TaJLw</recordid><startdate>20150219</startdate><enddate>20150219</enddate><creator>Ifeanyi, Casmir Ifeanyichukwu Cajetan</creator><creator>Ikeneche, Nkiruka Florence</creator><creator>Bassey, Bassey Enya</creator><creator>Al-Gallas, Nazek</creator><creator>Ben Aissa, Ridha</creator><creator>Boudabous, Abdellatif</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150219</creationdate><title>Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria</title><author>Ifeanyi, Casmir Ifeanyichukwu Cajetan ; Ikeneche, Nkiruka Florence ; Bassey, Bassey Enya ; Al-Gallas, Nazek ; Ben Aissa, Ridha ; Boudabous, Abdellatif</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-abbe4e45d9c4f45ffec6d5b552c3ee45672c27f0b863d24b33352fd0ca90659d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacterial Adhesion</topic><topic>Child, Preschool</topic><topic>Diarrhea - microbiology</topic><topic>E coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - physiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Genotype</topic><topic>Hepatocytes - microbiology</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Nigeria</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Virulence</topic><topic>Virulence Factors - analysis</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ifeanyi, Casmir Ifeanyichukwu Cajetan</creatorcontrib><creatorcontrib>Ikeneche, Nkiruka Florence</creatorcontrib><creatorcontrib>Bassey, Bassey Enya</creatorcontrib><creatorcontrib>Al-Gallas, Nazek</creatorcontrib><creatorcontrib>Ben Aissa, Ridha</creatorcontrib><creatorcontrib>Boudabous, Abdellatif</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Public Health Database</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of infection in developing countries</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ifeanyi, Casmir Ifeanyichukwu Cajetan</au><au>Ikeneche, Nkiruka Florence</au><au>Bassey, Bassey Enya</au><au>Al-Gallas, Nazek</au><au>Ben Aissa, Ridha</au><au>Boudabous, Abdellatif</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria</atitle><jtitle>Journal of infection in developing countries</jtitle><addtitle>J Infect Dev Ctries</addtitle><date>2015-02-19</date><risdate>2015</risdate><volume>9</volume><issue>2</issue><spage>165</spage><epage>174</epage><pages>165-174</pages><issn>1972-2680</issn><issn>2036-6590</issn><eissn>1972-2680</eissn><abstract>Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown.
Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied. E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.
Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.
This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>25699491</pmid><doi>10.3855/jidc.5528</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Adhesion Child, Preschool Diarrhea - microbiology E coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - physiology Escherichia coli Infections - microbiology Feces - microbiology Female Genotype Hepatocytes - microbiology Humans Infant Infant, Newborn Male Nigeria Phenotype Polymerase Chain Reaction Virulence Virulence Factors - analysis Virulence Factors - genetics |
| title | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
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