Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats
We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increa...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1998-05, Vol.246 (2), p.414-417 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Misugi, Emi Tojo, Shinichiro J. Yasuda, Taeko Kurata, Yukiko Morooka, Shigeaki |
| description | We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gel-filtration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat. |
| doi_str_mv | 10.1006/bbrc.1998.8634 |
| format | Article |
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Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gel-filtration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1998.8634</identifier><identifier>PMID: 9610374</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Binding, Competitive ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - standards ; Injections, Intravenous ; Kinetics ; Lipopolysaccharides - administration & dosage ; Lipopolysaccharides - toxicity ; Male ; P-Selectin - biosynthesis ; P-Selectin - blood ; Rats ; Rats, Sprague-Dawley ; Reference Standards ; Solubility</subject><ispartof>Biochemical and biophysical research communications, 1998-05, Vol.246 (2), p.414-417</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-2333dfea50cc9829e39efa57885507980bcccc433c07555476b64daa2f5580163</citedby><cites>FETCH-LOGICAL-c370t-2333dfea50cc9829e39efa57885507980bcccc433c07555476b64daa2f5580163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X9898634X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9610374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Misugi, Emi</creatorcontrib><creatorcontrib>Tojo, Shinichiro J.</creatorcontrib><creatorcontrib>Yasuda, Taeko</creatorcontrib><creatorcontrib>Kurata, Yukiko</creatorcontrib><creatorcontrib>Morooka, Shigeaki</creatorcontrib><title>Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gel-filtration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Disease Models, Animal</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - standards</subject><subject>Injections, Intravenous</subject><subject>Kinetics</subject><subject>Lipopolysaccharides - administration & dosage</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Male</subject><subject>P-Selectin - biosynthesis</subject><subject>P-Selectin - blood</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Standards</subject><subject>Solubility</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LxDAQxYMouq5evQk9ees6aZo0Ocqy6oLg4hfeQppOIdKmmnTF_e_Nsos3h8AMeW8eyY-QCwozCiCu6zrYGVVKzqRg5QGZUFCQFxTKQzKB5MgLRd9PyGmMHwCUlkIdk2MlKLCqnJC3pbcBTcQmW3Um9iZb5c_YoR2dz5a-Wduk1Js0jsF8ox_WMbtpeuddTBejG3w2tNnCN8M4_KSVdJ7MGM_IUWu6iOf7PiWvt4uX-X3-8Hi3nN885JZVMOYFY6xp0XCwVslCIVPYGl5JyTlUSkJtU5WMWag452UlalE2xhQt5xKoYFNytcv9DMPXGuOoexctdp3xmJ6qqeAChJTJONsZbRhiDNjqz-B6Ezaagt6C1FuQegtSb0Gmhct98rrusfmz78klXe50TN_7dhh0tA59wuVCoqebwf0X_QvDxIG7</recordid><startdate>19980519</startdate><enddate>19980519</enddate><creator>Misugi, Emi</creator><creator>Tojo, Shinichiro J.</creator><creator>Yasuda, Taeko</creator><creator>Kurata, Yukiko</creator><creator>Morooka, Shigeaki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19980519</creationdate><title>Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats</title><author>Misugi, Emi ; Tojo, Shinichiro J. ; Yasuda, Taeko ; Kurata, Yukiko ; Morooka, Shigeaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-2333dfea50cc9829e39efa57885507980bcccc433c07555476b64daa2f5580163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Disease Models, Animal</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - standards</topic><topic>Injections, Intravenous</topic><topic>Kinetics</topic><topic>Lipopolysaccharides - administration & dosage</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Male</topic><topic>P-Selectin - biosynthesis</topic><topic>P-Selectin - blood</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Standards</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Misugi, Emi</creatorcontrib><creatorcontrib>Tojo, Shinichiro J.</creatorcontrib><creatorcontrib>Yasuda, Taeko</creatorcontrib><creatorcontrib>Kurata, Yukiko</creatorcontrib><creatorcontrib>Morooka, Shigeaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Misugi, Emi</au><au>Tojo, Shinichiro J.</au><au>Yasuda, Taeko</au><au>Kurata, Yukiko</au><au>Morooka, Shigeaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1998-05-19</date><risdate>1998</risdate><volume>246</volume><issue>2</issue><spage>414</spage><epage>417</epage><pages>414-417</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. 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| ispartof | Biochemical and biophysical research communications, 1998-05, Vol.246 (2), p.414-417 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Binding, Competitive Disease Models, Animal Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - standards Injections, Intravenous Kinetics Lipopolysaccharides - administration & dosage Lipopolysaccharides - toxicity Male P-Selectin - biosynthesis P-Selectin - blood Rats Rats, Sprague-Dawley Reference Standards Solubility |
| title | Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats |
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