AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5
Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been...
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| Veröffentlicht in: | Plant, cell and environment cell and environment, 2015-03, Vol.38 (3), p.559-571 |
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| creator | KIM, JUN HYEOK HYUN, WOO YOUNG NGUYEN, HOAI NGUYEN JEONG, CHAN YOUNG XIONG, LIMING HONG, SUK‐WHAN LEE, HOJOUNG |
| description | Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT‐PCR to determine the levels of stress‐response gene transcripts in wild‐type and atmyb7 plants. We showed that Arabidopsis AtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high‐salt stress conditions, atmyb7 plants showed a lower germination rate than did wild‐type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis. |
| doi_str_mv | 10.1111/pce.12415 |
| format | Article |
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The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT‐PCR to determine the levels of stress‐response gene transcripts in wild‐type and atmyb7 plants. We showed that Arabidopsis AtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high‐salt stress conditions, atmyb7 plants showed a lower germination rate than did wild‐type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/pce.12415</identifier><identifier>PMID: 25053018</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>United States: Blackwell Scientific Publications</publisher><subject>abiotic stress ; abscisic acid ; Abscisic Acid - metabolism ; Abscisic Acid - pharmacology ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Basic-Leucine Zipper Transcription Factors - genetics ; Basic-Leucine Zipper Transcription Factors - metabolism ; gene expression ; Gene Expression Regulation, Plant - drug effects ; Genes, Reporter ; Germination - drug effects ; imbibition ; messenger RNA ; metabolism ; Mutation ; MYB transcription factor ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; proteins ; reverse transcriptase polymerase chain reaction ; seed germination ; seeds ; Seeds - genetics ; Seeds - physiology ; Signal Transduction ; Sodium Chloride - pharmacology ; stress response ; Stress, Physiological ; transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Plant, cell and environment, 2015-03, Vol.38 (3), p.559-571</ispartof><rights>2014 John Wiley & Sons Ltd</rights><rights>2014 John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpce.12415$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpce.12415$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25053018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, JUN HYEOK</creatorcontrib><creatorcontrib>HYUN, WOO YOUNG</creatorcontrib><creatorcontrib>NGUYEN, HOAI NGUYEN</creatorcontrib><creatorcontrib>JEONG, CHAN YOUNG</creatorcontrib><creatorcontrib>XIONG, LIMING</creatorcontrib><creatorcontrib>HONG, SUK‐WHAN</creatorcontrib><creatorcontrib>LEE, HOJOUNG</creatorcontrib><title>AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT‐PCR to determine the levels of stress‐response gene transcripts in wild‐type and atmyb7 plants. We showed that Arabidopsis AtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high‐salt stress conditions, atmyb7 plants showed a lower germination rate than did wild‐type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.</description><subject>abiotic stress</subject><subject>abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Abscisic Acid - pharmacology</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Basic-Leucine Zipper Transcription Factors - genetics</subject><subject>Basic-Leucine Zipper Transcription Factors - metabolism</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes, Reporter</subject><subject>Germination - drug effects</subject><subject>imbibition</subject><subject>messenger RNA</subject><subject>metabolism</subject><subject>Mutation</subject><subject>MYB transcription factor</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>proteins</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>seed germination</subject><subject>seeds</subject><subject>Seeds - genetics</subject><subject>Seeds - physiology</subject><subject>Signal Transduction</subject><subject>Sodium Chloride - pharmacology</subject><subject>stress response</subject><subject>Stress, Physiological</subject><subject>transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9u1DAQxi0EokvhwAuApV44NK0njvPnuKxKWamIqtALF8tOxqlLNgl20jY3HqGvwKvxJHizCwfmMqPPv8-25iPkNbATCHXal3gCcQLiCVkAT0XEWcKekgWDhEVZVsABeeH9LWNByIrn5CAWTHAG-YL8Wg6fJp0dU0X9qGvXjT1N6FV8xWnQj2mLtRrsHTYTdViPjRrQ0-X75e-fj7atxhIratsbq-1gu5Z2hnoMUo1uY1s1a3qiuunK77at6XCDFB96h97v8a2iv60v6eBU60tn-9lkVDl0Ljy0Fi_JM6Maj6_2_ZBcfzj7uvoYXXw-X6-WF5HhGReRSRWgKSqe6DRlRZIwgLJkCIpp0CyMRVKiKKo4zqvCaA4m04mOKzAsB8H5IXm3u7d33Y8R_SA31pfYNKrFbvQSUiGysDjYokf_obfd6Nrwu5liQkAOgXqzp0a9wUr2zm6Um-Tf5QfgdAfc2wanf-fA5DZVGVKVc6rycnU2D8HxducwqpOqdtbL6y8xAxGSzTMhcv4HcxWdfg</recordid><startdate>201503</startdate><enddate>201503</enddate><creator>KIM, JUN HYEOK</creator><creator>HYUN, WOO YOUNG</creator><creator>NGUYEN, HOAI NGUYEN</creator><creator>JEONG, CHAN YOUNG</creator><creator>XIONG, LIMING</creator><creator>HONG, SUK‐WHAN</creator><creator>LEE, HOJOUNG</creator><general>Blackwell Scientific Publications</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201503</creationdate><title>AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5</title><author>KIM, JUN HYEOK ; HYUN, WOO YOUNG ; NGUYEN, HOAI NGUYEN ; JEONG, CHAN YOUNG ; XIONG, LIMING ; HONG, SUK‐WHAN ; LEE, HOJOUNG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3735-f6a1ef9d34b660944011cc0e1a0b1b0cc094ce59d228d9fb31f7b4b2d1f081533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>abiotic stress</topic><topic>abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Abscisic Acid - pharmacology</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Basic-Leucine Zipper Transcription Factors - genetics</topic><topic>Basic-Leucine Zipper Transcription Factors - metabolism</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes, Reporter</topic><topic>Germination - drug effects</topic><topic>imbibition</topic><topic>messenger RNA</topic><topic>metabolism</topic><topic>Mutation</topic><topic>MYB transcription factor</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>proteins</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>seed germination</topic><topic>seeds</topic><topic>Seeds - genetics</topic><topic>Seeds - physiology</topic><topic>Signal Transduction</topic><topic>Sodium Chloride - pharmacology</topic><topic>stress response</topic><topic>Stress, Physiological</topic><topic>transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIM, JUN HYEOK</creatorcontrib><creatorcontrib>HYUN, WOO YOUNG</creatorcontrib><creatorcontrib>NGUYEN, HOAI NGUYEN</creatorcontrib><creatorcontrib>JEONG, CHAN YOUNG</creatorcontrib><creatorcontrib>XIONG, LIMING</creatorcontrib><creatorcontrib>HONG, SUK‐WHAN</creatorcontrib><creatorcontrib>LEE, HOJOUNG</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIM, JUN HYEOK</au><au>HYUN, WOO YOUNG</au><au>NGUYEN, HOAI NGUYEN</au><au>JEONG, CHAN YOUNG</au><au>XIONG, LIMING</au><au>HONG, SUK‐WHAN</au><au>LEE, HOJOUNG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2015-03</date><risdate>2015</risdate><volume>38</volume><issue>3</issue><spage>559</spage><epage>571</epage><pages>559-571</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT‐PCR to determine the levels of stress‐response gene transcripts in wild‐type and atmyb7 plants. We showed that Arabidopsis AtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high‐salt stress conditions, atmyb7 plants showed a lower germination rate than did wild‐type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.</abstract><cop>United States</cop><pub>Blackwell Scientific Publications</pub><pmid>25053018</pmid><doi>10.1111/pce.12415</doi><tpages>13</tpages></addata></record> |
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| subjects | abiotic stress abscisic acid Abscisic Acid - metabolism Abscisic Acid - pharmacology Arabidopsis Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Basic-Leucine Zipper Transcription Factors - genetics Basic-Leucine Zipper Transcription Factors - metabolism gene expression Gene Expression Regulation, Plant - drug effects Genes, Reporter Germination - drug effects imbibition messenger RNA metabolism Mutation MYB transcription factor Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plants, Genetically Modified Promoter Regions, Genetic - genetics proteins reverse transcriptase polymerase chain reaction seed germination seeds Seeds - genetics Seeds - physiology Signal Transduction Sodium Chloride - pharmacology stress response Stress, Physiological transcription factors Transcription Factors - genetics Transcription Factors - metabolism |
| title | AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA‐induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5 |
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