Genetic identification of asteroid larvae from Tasmania, Australia, by PCR-RFLP

Studies of seastar larvae in the Derwent River (Tasmania) were hampered by identification uncertainties. A genetic test was developed, based on PCR amplification of a 1300 bp mitochondrial DNA region followed by digestion with three restriction enzymes. The restriction profiles of adults of 14 seast...

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Veröffentlicht in:	Molecular ecology 1998-08, Vol.7 (8), p.1077-1082
Hauptverfasser:	EVANS, B. S., WHITE, R. W. G., WARD, R. D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Asterias amurensis asteroid larvae Coscinasterias muricata DNA species identification echinoderms Marine mitochondrial DNA Patiriella regularis
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container_title	Molecular ecology
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creator	EVANS, B. S. WHITE, R. W. G. WARD, R. D.
description	Studies of seastar larvae in the Derwent River (Tasmania) were hampered by identification uncertainties. A genetic test was developed, based on PCR amplification of a 1300 bp mitochondrial DNA region followed by digestion with three restriction enzymes. The restriction profiles of adults of 14 seastar species were determined. The test was validated for larvae as laboratory‐raised larvae of two species had the appropriate composite haplotypes. Approximately 80% of planktotrophic seastar larvae from Derwent River plankton samples were identified as the recently introduced northern Pacific seastar, Asterias amurensis. The two other larval seastars identified were Coscinasterias muricata and Patiriella regularis.
doi_str_mv	10.1046/j.1365-294x.1998.00395.x
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The two other larval seastars identified were Coscinasterias muricata and Patiriella regularis.</description><subject>Asterias amurensis</subject><subject>asteroid larvae</subject><subject>Coscinasterias muricata</subject><subject>DNA species identification</subject><subject>echinoderms</subject><subject>Marine</subject><subject>mitochondrial DNA</subject><subject>Patiriella regularis</subject><issn>0962-1083</issn><issn>1365-294X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqNkMtOwzAQRS0EEqXwD16xIsFOYieR2FRRH4jSVqWIio3lOLbkkkexU0j_noQAa2ZzrzRzZnEAgBi5GAX0dudinxLHi4PGxXEcuQj5MXGbEzD4XWxPwQDF1HMwivxzcGHtDiHse4QMwHIqS1lrAXUmy1orLXitqxJWCnJbS1PpDObcfHAJlakKuOG24KXmN3B0sLXheVfTI1wla2c9ma8uwZniuZVXPzkEz5PxJpk58-X0PhnNHREEhDiRSMOYUkGoQpkSWFEpKYlUKASJQj9LCfEQx5FKERVxoNI4DDlpM-OqXSB_CK77v3tTvR-krVmhrZB5zktZHSzDlLQTeu1h1B8KU1lrpGJ7owtujgwj1hlkO9aJYp1B1hlk3wZZ06J3Pfqpc3n8N8cex0lbWtzpcd2KbP5wbt4YDf2QsJfFlD1M1tvFUzJjr_4XMeyHTQ</recordid><startdate>199808</startdate><enddate>199808</enddate><creator>EVANS, B. 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subjects	Asterias amurensis asteroid larvae Coscinasterias muricata DNA species identification echinoderms Marine mitochondrial DNA Patiriella regularis
title	Genetic identification of asteroid larvae from Tasmania, Australia, by PCR-RFLP
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