Secretion of an active recombinant dog gastric lipase from baculovirus-infected insect cells
An active dog gastric lipase (DGL) belonging to the acid-stable mammalian lipase family, was produced and secreted from baculovirus-infected insect cells using the honeybee melittin and the DGL signal sequences. Both sequences drove the secretion of an active 47 kDa recombinant DGL (rDGL). rDGL was...
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| Veröffentlicht in: | Biotechnology letters 1998-07, Vol.20 (7), p.697-702 |
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| creator | JOLIFF, G VAGANAY, S LEGAY, C BENICOURT, C |
| description | An active dog gastric lipase (DGL) belonging to the acid-stable mammalian lipase family, was produced and secreted from baculovirus-infected insect cells using the honeybee melittin and the DGL signal sequences. Both sequences drove the secretion of an active 47 kDa recombinant DGL (rDGL). rDGL was secreted at about 35 mg/L of culture medium. A one step purification using a cation exchange chromatography was used to recover an active electrophoretically pure rDGL from 2 days post-infection supernatant. There were not significant differences between the enzymatic properties of native and recombinant proteins. © Rapid Science Ltd. 1998[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/A:1005330826017 |
| format | Article |
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Both sequences drove the secretion of an active 47 kDa recombinant DGL (rDGL). rDGL was secreted at about 35 mg/L of culture medium. A one step purification using a cation exchange chromatography was used to recover an active electrophoretically pure rDGL from 2 days post-infection supernatant. There were not significant differences between the enzymatic properties of native and recombinant proteins. © Rapid Science Ltd. 1998[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1005330826017</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Apis mellifera ; Biological and medical sciences ; Biotechnology ; Cation exchange ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Insecta ; Insects ; Methods. Procedures. 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| subjects | Apis mellifera Biological and medical sciences Biotechnology Cation exchange Enzymes Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Insecta Insects Methods. Procedures. Technologies Modification of gene expression level Proteins |
| title | Secretion of an active recombinant dog gastric lipase from baculovirus-infected insect cells |
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