BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro
OBJECTIVES Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their roles in thoracic aortic aneurysms. We investigated BRG1 expression in thoracic aortic aneurysms and the roles of BRG1 and the lncRNA HIF 1...
Gespeichert in:
| Veröffentlicht in: | European journal of cardio-thoracic surgery 2015-03, Vol.47 (3), p.439-446 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 446 |
|---|---|
| container_issue | 3 |
| container_start_page | 439 |
| container_title | European journal of cardio-thoracic surgery |
| container_volume | 47 |
| creator | Wang, Shuwei Zhang, Xiwu Yuan, Yang Tan, Mengwei Zhang, Le Xue, Xiang Yan, Yan Han, Lin Xu, Zhiyun |
| description | OBJECTIVES
Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their roles in thoracic aortic aneurysms. We investigated BRG1 expression in thoracic aortic aneurysms and the roles of BRG1 and the lncRNA HIF 1 alpha-antisense RNA 1 in regulating the proliferation and apoptosis of aortic smooth muscle cells in vitro.
METHODS
BRG1 mRNA and protein expression in human aortic media specimens were examined by quantitative real-time polymerase chain reaction, immunohistochemical staining and western blot. BRG1 expression was up-regulated by lentiviral vectors. Vascular smooth muscle cell proliferation and apoptosis were studied using Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick-end labelling assays. We performed western blots to detect Caspase3 and Bcl2 protein expression. LncRNAs regulated by BRG1 were identified through microarray in BRG1 gain- and loss-of-function vascular smooth muscle cells. Finally, the expression of HIF 1 alpha-antisense RNA 1 was reduced by siRNA and cell proliferation and apoptosis was studied using Cell Counting Kit-8 assays, caspase-3 activity assays and western blot.
RESULTS
BRG1 expression in the aortic media was significantly higher in thoracic aortic aneurysms than in normal controls. Overexpression of BRG1 in human aortic smooth muscle cells promoted apoptosis and reduced proliferation. The expression of HIF 1 alpha-antisense RNA 1 was significantly down- and up-regulated in BRG1 knock-down and overexpressing vascular smooth muscle cells, respectively. We further demonstrated that suppression of HIF 1 alpha-antisense RNA 1 by siRNA in vascular smooth muscle cells reduced apoptosis and promoted proliferation.
CONCLUSIONS
BRG1 is overexpressed in the aortic media of thoracic aortic aneurysms and the interaction between BRG1 and HIF 1 alpha-antisense RNA 1 plays a key role in the proliferation and apoptosis of vascular smooth muscle cells in vitro, which may contribute to the pathogenesis of thoracic aortic aneurysms. |
| doi_str_mv | 10.1093/ejcts/ezu215 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1655256311</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/ejcts/ezu215</oup_id><sourcerecordid>1655256311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-18de2744254d28cca8a70cdd065cb9dcd2376d3edd4f0290d22567817098f2693</originalsourceid><addsrcrecordid>eNp9kU9vFSEUxYnR2FrduTbsdOFYYJiBWT4b-ydpNKmauJtQuPMezcwwcqGxfjA_X5m-6tLVOQk_DvdyCHnN2QfOuvoYbmzCY_idBW-ekEOuVV2pWv54WjzjrFKdZAfkBeINY6ythXpODoTUqtFaHpI_H6_OOIVfSwREH2bqkfrZRjAIrjiadiEa6y01IaZVZsjxDicsztEI2zyaBEiXGEY_QDRpTVnPzBKWFLAEhoHeGrSFjBSnENKOThntCNTCOGJ5I4a83RUFOoZ5S-cwVzY4X-zV5w09vzjlm2rzla8D3foUw0vybDAjwqtHPSLfTz99OzmvLr-cXZxsLisrhUoV1w6EklI00gltrdFGMescaxt73TnrRK1aV4NzcmCiY06IplWaK9bpQbRdfUTe7XPLej8zYOonj-vQ5RtCxp63TVOu1JwX9P0etTEgRhj6JfrJxLues35tqn9oqt83VfA3j8n5egL3D_5bTQHe7oGQl_9H3QP3_aFH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1655256311</pqid></control><display><type>article</type><title>BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Wang, Shuwei ; Zhang, Xiwu ; Yuan, Yang ; Tan, Mengwei ; Zhang, Le ; Xue, Xiang ; Yan, Yan ; Han, Lin ; Xu, Zhiyun</creator><creatorcontrib>Wang, Shuwei ; Zhang, Xiwu ; Yuan, Yang ; Tan, Mengwei ; Zhang, Le ; Xue, Xiang ; Yan, Yan ; Han, Lin ; Xu, Zhiyun</creatorcontrib><description>OBJECTIVES
Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their roles in thoracic aortic aneurysms. We investigated BRG1 expression in thoracic aortic aneurysms and the roles of BRG1 and the lncRNA HIF 1 alpha-antisense RNA 1 in regulating the proliferation and apoptosis of aortic smooth muscle cells in vitro.
METHODS
BRG1 mRNA and protein expression in human aortic media specimens were examined by quantitative real-time polymerase chain reaction, immunohistochemical staining and western blot. BRG1 expression was up-regulated by lentiviral vectors. Vascular smooth muscle cell proliferation and apoptosis were studied using Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick-end labelling assays. We performed western blots to detect Caspase3 and Bcl2 protein expression. LncRNAs regulated by BRG1 were identified through microarray in BRG1 gain- and loss-of-function vascular smooth muscle cells. Finally, the expression of HIF 1 alpha-antisense RNA 1 was reduced by siRNA and cell proliferation and apoptosis was studied using Cell Counting Kit-8 assays, caspase-3 activity assays and western blot.
RESULTS
BRG1 expression in the aortic media was significantly higher in thoracic aortic aneurysms than in normal controls. Overexpression of BRG1 in human aortic smooth muscle cells promoted apoptosis and reduced proliferation. The expression of HIF 1 alpha-antisense RNA 1 was significantly down- and up-regulated in BRG1 knock-down and overexpressing vascular smooth muscle cells, respectively. We further demonstrated that suppression of HIF 1 alpha-antisense RNA 1 by siRNA in vascular smooth muscle cells reduced apoptosis and promoted proliferation.
CONCLUSIONS
BRG1 is overexpressed in the aortic media of thoracic aortic aneurysms and the interaction between BRG1 and HIF 1 alpha-antisense RNA 1 plays a key role in the proliferation and apoptosis of vascular smooth muscle cells in vitro, which may contribute to the pathogenesis of thoracic aortic aneurysms.</description><identifier>ISSN: 1010-7940</identifier><identifier>EISSN: 1873-734X</identifier><identifier>DOI: 10.1093/ejcts/ezu215</identifier><identifier>PMID: 24875884</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><subject>Aorta, Thoracic - chemistry ; Aorta, Thoracic - metabolism ; Aortic Aneurysm, Thoracic - metabolism ; Apoptosis - genetics ; Cell Line ; Cell Proliferation - genetics ; DNA Helicases - analysis ; DNA Helicases - genetics ; DNA Helicases - metabolism ; Gene Silencing ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit - genetics ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - metabolism ; Nuclear Proteins - analysis ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; RNA, Long Noncoding - metabolism ; Transcription Factors - analysis ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>European journal of cardio-thoracic surgery, 2015-03, Vol.47 (3), p.439-446</ispartof><rights>The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. 2014</rights><rights>The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-18de2744254d28cca8a70cdd065cb9dcd2376d3edd4f0290d22567817098f2693</citedby><cites>FETCH-LOGICAL-c427t-18de2744254d28cca8a70cdd065cb9dcd2376d3edd4f0290d22567817098f2693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24875884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Shuwei</creatorcontrib><creatorcontrib>Zhang, Xiwu</creatorcontrib><creatorcontrib>Yuan, Yang</creatorcontrib><creatorcontrib>Tan, Mengwei</creatorcontrib><creatorcontrib>Zhang, Le</creatorcontrib><creatorcontrib>Xue, Xiang</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Han, Lin</creatorcontrib><creatorcontrib>Xu, Zhiyun</creatorcontrib><title>BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro</title><title>European journal of cardio-thoracic surgery</title><addtitle>Eur J Cardiothorac Surg</addtitle><description>OBJECTIVES
Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their roles in thoracic aortic aneurysms. We investigated BRG1 expression in thoracic aortic aneurysms and the roles of BRG1 and the lncRNA HIF 1 alpha-antisense RNA 1 in regulating the proliferation and apoptosis of aortic smooth muscle cells in vitro.
METHODS
BRG1 mRNA and protein expression in human aortic media specimens were examined by quantitative real-time polymerase chain reaction, immunohistochemical staining and western blot. BRG1 expression was up-regulated by lentiviral vectors. Vascular smooth muscle cell proliferation and apoptosis were studied using Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick-end labelling assays. We performed western blots to detect Caspase3 and Bcl2 protein expression. LncRNAs regulated by BRG1 were identified through microarray in BRG1 gain- and loss-of-function vascular smooth muscle cells. Finally, the expression of HIF 1 alpha-antisense RNA 1 was reduced by siRNA and cell proliferation and apoptosis was studied using Cell Counting Kit-8 assays, caspase-3 activity assays and western blot.
RESULTS
BRG1 expression in the aortic media was significantly higher in thoracic aortic aneurysms than in normal controls. Overexpression of BRG1 in human aortic smooth muscle cells promoted apoptosis and reduced proliferation. The expression of HIF 1 alpha-antisense RNA 1 was significantly down- and up-regulated in BRG1 knock-down and overexpressing vascular smooth muscle cells, respectively. We further demonstrated that suppression of HIF 1 alpha-antisense RNA 1 by siRNA in vascular smooth muscle cells reduced apoptosis and promoted proliferation.
CONCLUSIONS
BRG1 is overexpressed in the aortic media of thoracic aortic aneurysms and the interaction between BRG1 and HIF 1 alpha-antisense RNA 1 plays a key role in the proliferation and apoptosis of vascular smooth muscle cells in vitro, which may contribute to the pathogenesis of thoracic aortic aneurysms.</description><subject>Aorta, Thoracic - chemistry</subject><subject>Aorta, Thoracic - metabolism</subject><subject>Aortic Aneurysm, Thoracic - metabolism</subject><subject>Apoptosis - genetics</subject><subject>Cell Line</subject><subject>Cell Proliferation - genetics</subject><subject>DNA Helicases - analysis</subject><subject>DNA Helicases - genetics</subject><subject>DNA Helicases - metabolism</subject><subject>Gene Silencing</subject><subject>Humans</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Nuclear Proteins - analysis</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>1010-7940</issn><issn>1873-734X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vFSEUxYnR2FrduTbsdOFYYJiBWT4b-ydpNKmauJtQuPMezcwwcqGxfjA_X5m-6tLVOQk_DvdyCHnN2QfOuvoYbmzCY_idBW-ekEOuVV2pWv54WjzjrFKdZAfkBeINY6ythXpODoTUqtFaHpI_H6_OOIVfSwREH2bqkfrZRjAIrjiadiEa6y01IaZVZsjxDicsztEI2zyaBEiXGEY_QDRpTVnPzBKWFLAEhoHeGrSFjBSnENKOThntCNTCOGJ5I4a83RUFOoZ5S-cwVzY4X-zV5w09vzjlm2rzla8D3foUw0vybDAjwqtHPSLfTz99OzmvLr-cXZxsLisrhUoV1w6EklI00gltrdFGMescaxt73TnrRK1aV4NzcmCiY06IplWaK9bpQbRdfUTe7XPLej8zYOonj-vQ5RtCxp63TVOu1JwX9P0etTEgRhj6JfrJxLues35tqn9oqt83VfA3j8n5egL3D_5bTQHe7oGQl_9H3QP3_aFH</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Wang, Shuwei</creator><creator>Zhang, Xiwu</creator><creator>Yuan, Yang</creator><creator>Tan, Mengwei</creator><creator>Zhang, Le</creator><creator>Xue, Xiang</creator><creator>Yan, Yan</creator><creator>Han, Lin</creator><creator>Xu, Zhiyun</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150301</creationdate><title>BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro</title><author>Wang, Shuwei ; Zhang, Xiwu ; Yuan, Yang ; Tan, Mengwei ; Zhang, Le ; Xue, Xiang ; Yan, Yan ; Han, Lin ; Xu, Zhiyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-18de2744254d28cca8a70cdd065cb9dcd2376d3edd4f0290d22567817098f2693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aorta, Thoracic - chemistry</topic><topic>Aorta, Thoracic - metabolism</topic><topic>Aortic Aneurysm, Thoracic - metabolism</topic><topic>Apoptosis - genetics</topic><topic>Cell Line</topic><topic>Cell Proliferation - genetics</topic><topic>DNA Helicases - analysis</topic><topic>DNA Helicases - genetics</topic><topic>DNA Helicases - metabolism</topic><topic>Gene Silencing</topic><topic>Humans</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Nuclear Proteins - analysis</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Transcription Factors - analysis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Shuwei</creatorcontrib><creatorcontrib>Zhang, Xiwu</creatorcontrib><creatorcontrib>Yuan, Yang</creatorcontrib><creatorcontrib>Tan, Mengwei</creatorcontrib><creatorcontrib>Zhang, Le</creatorcontrib><creatorcontrib>Xue, Xiang</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Han, Lin</creatorcontrib><creatorcontrib>Xu, Zhiyun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cardio-thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shuwei</au><au>Zhang, Xiwu</au><au>Yuan, Yang</au><au>Tan, Mengwei</au><au>Zhang, Le</au><au>Xue, Xiang</au><au>Yan, Yan</au><au>Han, Lin</au><au>Xu, Zhiyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro</atitle><jtitle>European journal of cardio-thoracic surgery</jtitle><addtitle>Eur J Cardiothorac Surg</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>47</volume><issue>3</issue><spage>439</spage><epage>446</epage><pages>439-446</pages><issn>1010-7940</issn><eissn>1873-734X</eissn><abstract>OBJECTIVES
Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their roles in thoracic aortic aneurysms. We investigated BRG1 expression in thoracic aortic aneurysms and the roles of BRG1 and the lncRNA HIF 1 alpha-antisense RNA 1 in regulating the proliferation and apoptosis of aortic smooth muscle cells in vitro.
METHODS
BRG1 mRNA and protein expression in human aortic media specimens were examined by quantitative real-time polymerase chain reaction, immunohistochemical staining and western blot. BRG1 expression was up-regulated by lentiviral vectors. Vascular smooth muscle cell proliferation and apoptosis were studied using Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick-end labelling assays. We performed western blots to detect Caspase3 and Bcl2 protein expression. LncRNAs regulated by BRG1 were identified through microarray in BRG1 gain- and loss-of-function vascular smooth muscle cells. Finally, the expression of HIF 1 alpha-antisense RNA 1 was reduced by siRNA and cell proliferation and apoptosis was studied using Cell Counting Kit-8 assays, caspase-3 activity assays and western blot.
RESULTS
BRG1 expression in the aortic media was significantly higher in thoracic aortic aneurysms than in normal controls. Overexpression of BRG1 in human aortic smooth muscle cells promoted apoptosis and reduced proliferation. The expression of HIF 1 alpha-antisense RNA 1 was significantly down- and up-regulated in BRG1 knock-down and overexpressing vascular smooth muscle cells, respectively. We further demonstrated that suppression of HIF 1 alpha-antisense RNA 1 by siRNA in vascular smooth muscle cells reduced apoptosis and promoted proliferation.
CONCLUSIONS
BRG1 is overexpressed in the aortic media of thoracic aortic aneurysms and the interaction between BRG1 and HIF 1 alpha-antisense RNA 1 plays a key role in the proliferation and apoptosis of vascular smooth muscle cells in vitro, which may contribute to the pathogenesis of thoracic aortic aneurysms.</abstract><cop>Germany</cop><pub>Oxford University Press</pub><pmid>24875884</pmid><doi>10.1093/ejcts/ezu215</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1010-7940 |
| ispartof | European journal of cardio-thoracic surgery, 2015-03, Vol.47 (3), p.439-446 |
| issn | 1010-7940 1873-734X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1655256311 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aorta, Thoracic - chemistry Aorta, Thoracic - metabolism Aortic Aneurysm, Thoracic - metabolism Apoptosis - genetics Cell Line Cell Proliferation - genetics DNA Helicases - analysis DNA Helicases - genetics DNA Helicases - metabolism Gene Silencing Humans Hypoxia-Inducible Factor 1, alpha Subunit - genetics Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - metabolism Nuclear Proteins - analysis Nuclear Proteins - genetics Nuclear Proteins - metabolism RNA, Long Noncoding - metabolism Transcription Factors - analysis Transcription Factors - genetics Transcription Factors - metabolism |
| title | BRG1 expression is increased in thoracic aortic aneurysms and regulates proliferation and apoptosis of vascular smooth muscle cells through the long non-coding RNA HIF1A-AS1 in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T09%3A25%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=BRG1%20expression%20is%20increased%20in%20thoracic%20aortic%20aneurysms%20and%20regulates%20proliferation%20and%20apoptosis%20of%20vascular%20smooth%20muscle%20cells%20through%20the%20long%20non-coding%20RNA%20HIF1A-AS1%20in%20vitro&rft.jtitle=European%20journal%20of%20cardio-thoracic%20surgery&rft.au=Wang,%20Shuwei&rft.date=2015-03-01&rft.volume=47&rft.issue=3&rft.spage=439&rft.epage=446&rft.pages=439-446&rft.issn=1010-7940&rft.eissn=1873-734X&rft_id=info:doi/10.1093/ejcts/ezu215&rft_dat=%3Cproquest_cross%3E1655256311%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1655256311&rft_id=info:pmid/24875884&rft_oup_id=10.1093/ejcts/ezu215&rfr_iscdi=true |