2-Nitroacetanilide as substrate for determination of aryl acylamidase activity in soils
A direct colorimetric assay for measuring aryl acylamidase activity in soils is described using 2-nitroacetanilide (2-NAA) as substrate. The assay is based upon an increase in absorption (410 nm) due to formation of the chromogenic hydrolysis product 2-nitroaniline, after incubation of soil with 2-N...
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| Veröffentlicht in: | Soil biology & biochemistry 1998-06, Vol.30 (6), p.679-686 |
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| creator | Zablotowicz, R.M. Hoagland, R.E. Wagner, S.C. |
| description | A direct colorimetric assay for measuring aryl acylamidase activity in soils is described using 2-nitroacetanilide (2-NAA) as substrate. The assay is based upon an increase in absorption (410
nm) due to formation of the chromogenic hydrolysis product 2-nitroaniline, after incubation of soil with 2-NAA (2.0
m
m) in phosphate buffer (pH 8.0). Due to low specific activities (5–53
nmol
g
−1 soil h
−1) incubations of 20–24
h are required. 2-NAA hydrolytic activity in soil was dependent on substrate concentration, with apparent
K
m values of 180 to 920
μ
m for the soils examined. 2-NAA aryl acylamidase activity was linear for a 35
h incubation. The activity was reduced by 71 to 85% in the presence of carbaryl (100
μ
m), an aryl acylamidase inhibitor. Aryl acylamidase activity was increased from 1.31 to 2.78-fold in no-till (NT) soils compared to conventional-till (CT) soils, corresponding with increased organic matter accumulation and microbial activity in the four (NT) soils studied. |
| doi_str_mv | 10.1016/S0038-0717(97)00216-2 |
| format | Article |
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nm) due to formation of the chromogenic hydrolysis product 2-nitroaniline, after incubation of soil with 2-NAA (2.0
m
m) in phosphate buffer (pH 8.0). Due to low specific activities (5–53
nmol
g
−1 soil h
−1) incubations of 20–24
h are required. 2-NAA hydrolytic activity in soil was dependent on substrate concentration, with apparent
K
m values of 180 to 920
μ
m for the soils examined. 2-NAA aryl acylamidase activity was linear for a 35
h incubation. The activity was reduced by 71 to 85% in the presence of carbaryl (100
μ
m), an aryl acylamidase inhibitor. Aryl acylamidase activity was increased from 1.31 to 2.78-fold in no-till (NT) soils compared to conventional-till (CT) soils, corresponding with increased organic matter accumulation and microbial activity in the four (NT) soils studied.</description><identifier>ISSN: 0038-0717</identifier><identifier>EISSN: 1879-3428</identifier><identifier>DOI: 10.1016/S0038-0717(97)00216-2</identifier><identifier>CODEN: SBIOAH</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biochemistry ; Biochemistry and biology ; Biological and medical sciences ; Chemical, physicochemical, biochemical and biological properties ; Fundamental and applied biological sciences. Psychology ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; Soil science</subject><ispartof>Soil biology & biochemistry, 1998-06, Vol.30 (6), p.679-686</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-6249ea1b06fa1cd9432b87f7de6ff22f16892108619537d7fbf07c9532815f483</citedby><cites>FETCH-LOGICAL-c401t-6249ea1b06fa1cd9432b87f7de6ff22f16892108619537d7fbf07c9532815f483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0038-0717(97)00216-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2258466$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zablotowicz, R.M.</creatorcontrib><creatorcontrib>Hoagland, R.E.</creatorcontrib><creatorcontrib>Wagner, S.C.</creatorcontrib><title>2-Nitroacetanilide as substrate for determination of aryl acylamidase activity in soils</title><title>Soil biology & biochemistry</title><description>A direct colorimetric assay for measuring aryl acylamidase activity in soils is described using 2-nitroacetanilide (2-NAA) as substrate. The assay is based upon an increase in absorption (410
nm) due to formation of the chromogenic hydrolysis product 2-nitroaniline, after incubation of soil with 2-NAA (2.0
m
m) in phosphate buffer (pH 8.0). Due to low specific activities (5–53
nmol
g
−1 soil h
−1) incubations of 20–24
h are required. 2-NAA hydrolytic activity in soil was dependent on substrate concentration, with apparent
K
m values of 180 to 920
μ
m for the soils examined. 2-NAA aryl acylamidase activity was linear for a 35
h incubation. The activity was reduced by 71 to 85% in the presence of carbaryl (100
μ
m), an aryl acylamidase inhibitor. Aryl acylamidase activity was increased from 1.31 to 2.78-fold in no-till (NT) soils compared to conventional-till (CT) soils, corresponding with increased organic matter accumulation and microbial activity in the four (NT) soils studied.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biochemistry</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Soil science</subject><issn>0038-0717</issn><issn>1879-3428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMouH78BCEHET1Uk7RN0pOI-AWiBxWPIZtOYKTbaCYr7L-3uuLV08zheedlHsYOpDiVQuqzJyFqWwkjzXFnToRQUldqg82kNV1VN8pustkfss12iN7ERLWynrFXVT1gyckHKH7EAXvgnjgt51SyL8BjyryHAnmBoy-YRp4i93k1cB9Wg19g72mKhIKfWFYcR04JB9pjW9EPBPu_c5e9XF89X95W9483d5cX91VohCyVVk0HXs6Fjl6GvmtqNbcmmh50jEpFqW2npLBadm1tehPnUZgw7crKNja23mVH67vvOX0sgYpbIAUYBj9CWpKTum2M0N0Etmsw5ESUIbr3jIvpESeF-9bofjS6b0euM-5Ho1NT7vC3wFPwQ8x-DEh_YaVa22g9YedrDKZnPxGyo4AwBugxQyiuT_hP0RccCYa1</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Zablotowicz, R.M.</creator><creator>Hoagland, R.E.</creator><creator>Wagner, S.C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19980601</creationdate><title>2-Nitroacetanilide as substrate for determination of aryl acylamidase activity in soils</title><author>Zablotowicz, R.M. ; Hoagland, R.E. ; Wagner, S.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-6249ea1b06fa1cd9432b87f7de6ff22f16892108619537d7fbf07c9532815f483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biochemistry</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Soil science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zablotowicz, R.M.</creatorcontrib><creatorcontrib>Hoagland, R.E.</creatorcontrib><creatorcontrib>Wagner, S.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Soil biology & biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zablotowicz, R.M.</au><au>Hoagland, R.E.</au><au>Wagner, S.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>2-Nitroacetanilide as substrate for determination of aryl acylamidase activity in soils</atitle><jtitle>Soil biology & biochemistry</jtitle><date>1998-06-01</date><risdate>1998</risdate><volume>30</volume><issue>6</issue><spage>679</spage><epage>686</epage><pages>679-686</pages><issn>0038-0717</issn><eissn>1879-3428</eissn><coden>SBIOAH</coden><abstract>A direct colorimetric assay for measuring aryl acylamidase activity in soils is described using 2-nitroacetanilide (2-NAA) as substrate. The assay is based upon an increase in absorption (410
nm) due to formation of the chromogenic hydrolysis product 2-nitroaniline, after incubation of soil with 2-NAA (2.0
m
m) in phosphate buffer (pH 8.0). Due to low specific activities (5–53
nmol
g
−1 soil h
−1) incubations of 20–24
h are required. 2-NAA hydrolytic activity in soil was dependent on substrate concentration, with apparent
K
m values of 180 to 920
μ
m for the soils examined. 2-NAA aryl acylamidase activity was linear for a 35
h incubation. The activity was reduced by 71 to 85% in the presence of carbaryl (100
μ
m), an aryl acylamidase inhibitor. Aryl acylamidase activity was increased from 1.31 to 2.78-fold in no-till (NT) soils compared to conventional-till (CT) soils, corresponding with increased organic matter accumulation and microbial activity in the four (NT) soils studied.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0038-0717(97)00216-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Soil biology & biochemistry, 1998-06, Vol.30 (6), p.679-686 |
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| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Agronomy. Soil science and plant productions Biochemistry Biochemistry and biology Biological and medical sciences Chemical, physicochemical, biochemical and biological properties Fundamental and applied biological sciences. Psychology Physics, chemistry, biochemistry and biology of agricultural and forest soils Soil science |
| title | 2-Nitroacetanilide as substrate for determination of aryl acylamidase activity in soils |
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