Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization
This report describes the effect of overexpressing granulocyte macrophage colony stimulating factor (GMCSF) in breast cancer cells, which otherwise is involved in proliferation and differentiation of granulocyte and macrophage lineages. The purified recombinant GMCSF cytokine is known to exert dose-...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2015-02, Vol.175 (4), p.1948-1959 |
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| container_end_page | 1959 |
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| container_issue | 4 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Chaubey, Nidhi Ghosh, Siddhartha Sankar |
| description | This report describes the effect of overexpressing granulocyte macrophage colony stimulating factor (GMCSF) in breast cancer cells, which otherwise is involved in proliferation and differentiation of granulocyte and macrophage lineages. The purified recombinant GMCSF cytokine is known to exert dose-dependent proliferative response on various cancer cells, but its effect during overexpression is yet to be evaluated. In our present study, we have generated MCF-7 (breast cancer) cells overexpressing GMCSF. Interestingly, cell viability studies showed pronounced sensitivity of GMCSF overexpressing MCF-7 cells towards anticancer drugs, such as, doxorubicin, 5FU and cisplatin. These findings were substantiated by cell cycle analysis of the drug-treated GMCSF overexpressing MCF-7 cells. Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) results revealed differential expressions of cyclins, and the carboxyfluorescein succinimidyl ester (CFSE)-based assay established decrease in doubling time of GMCSF overexpressed cells with respect to the control populations. Thus, overexpressing of proliferative GMCSF cytokine in breast cancer cells may increase susceptibility to anticancer drugs. |
| doi_str_mv | 10.1007/s12010-014-1373-5 |
| format | Article |
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The purified recombinant GMCSF cytokine is known to exert dose-dependent proliferative response on various cancer cells, but its effect during overexpression is yet to be evaluated. In our present study, we have generated MCF-7 (breast cancer) cells overexpressing GMCSF. Interestingly, cell viability studies showed pronounced sensitivity of GMCSF overexpressing MCF-7 cells towards anticancer drugs, such as, doxorubicin, 5FU and cisplatin. These findings were substantiated by cell cycle analysis of the drug-treated GMCSF overexpressing MCF-7 cells. Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) results revealed differential expressions of cyclins, and the carboxyfluorescein succinimidyl ester (CFSE)-based assay established decrease in doubling time of GMCSF overexpressed cells with respect to the control populations. Thus, overexpressing of proliferative GMCSF cytokine in breast cancer cells may increase susceptibility to anticancer drugs.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-014-1373-5</identifier><identifier>PMID: 25432336</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Antineoplastic Agents - pharmacology ; Biochemistry ; Biotechnology ; Breast cancer ; breast neoplasms ; cell cycle ; Cell Cycle - drug effects ; Cell growth ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; cell viability ; Chemistry ; Chemistry and Materials Science ; cisplatin ; Cisplatin - pharmacology ; colony-stimulating factors ; cyclins ; Cyclins - genetics ; Cyclins - metabolism ; Cytokines ; dose response ; doxorubicin ; Doxorubicin - pharmacology ; Female ; Fluorouracil - pharmacology ; Gene Expression ; gene overexpression ; Granulocyte-Macrophage Colony-Stimulating Factor - genetics ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; Growth factors ; Humans ; Inhibitory Concentration 50 ; macrophages ; MCF-7 Cells ; neoplasm cells ; Plasmids - chemistry ; Plasmids - metabolism ; Protein expression ; reverse transcriptase polymerase chain reaction ; Transfection ; Transgenes</subject><ispartof>Applied biochemistry and biotechnology, 2015-02, Vol.175 (4), p.1948-1959</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-9e16b33889633735c9234283155aa0475e8bd6cfdcf0108dcfe72a105b7f58e23</citedby><cites>FETCH-LOGICAL-c396t-9e16b33889633735c9234283155aa0475e8bd6cfdcf0108dcfe72a105b7f58e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-014-1373-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-014-1373-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25432336$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaubey, Nidhi</creatorcontrib><creatorcontrib>Ghosh, Siddhartha Sankar</creatorcontrib><title>Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>This report describes the effect of overexpressing granulocyte macrophage colony stimulating factor (GMCSF) in breast cancer cells, which otherwise is involved in proliferation and differentiation of granulocyte and macrophage lineages. The purified recombinant GMCSF cytokine is known to exert dose-dependent proliferative response on various cancer cells, but its effect during overexpression is yet to be evaluated. In our present study, we have generated MCF-7 (breast cancer) cells overexpressing GMCSF. Interestingly, cell viability studies showed pronounced sensitivity of GMCSF overexpressing MCF-7 cells towards anticancer drugs, such as, doxorubicin, 5FU and cisplatin. These findings were substantiated by cell cycle analysis of the drug-treated GMCSF overexpressing MCF-7 cells. Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) results revealed differential expressions of cyclins, and the carboxyfluorescein succinimidyl ester (CFSE)-based assay established decrease in doubling time of GMCSF overexpressed cells with respect to the control populations. Thus, overexpressing of proliferative GMCSF cytokine in breast cancer cells may increase susceptibility to anticancer drugs.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>breast neoplasms</subject><subject>cell cycle</subject><subject>Cell Cycle - drug effects</subject><subject>Cell growth</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>cell viability</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>cisplatin</subject><subject>Cisplatin - pharmacology</subject><subject>colony-stimulating factors</subject><subject>cyclins</subject><subject>Cyclins - genetics</subject><subject>Cyclins - metabolism</subject><subject>Cytokines</subject><subject>dose response</subject><subject>doxorubicin</subject><subject>Doxorubicin - pharmacology</subject><subject>Female</subject><subject>Fluorouracil - pharmacology</subject><subject>Gene Expression</subject><subject>gene overexpression</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>macrophages</subject><subject>MCF-7 Cells</subject><subject>neoplasm cells</subject><subject>Plasmids - chemistry</subject><subject>Plasmids - metabolism</subject><subject>Protein expression</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Transfection</subject><subject>Transgenes</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtv1DAUhS0EokPhB7ABS2y6CfgRP7KE0BakQV1Mu7Y8npuQKmMPdgJMfz23SkGIBasr2d8593EIecnZW86YeVe4YJxVjNcVl0ZW6hFZcaWaiomGPyYrJvBRCNuckGel3DLGhVXmKTkRqpZCSr0id1ffIcPPQ4ZShhRp6uhl9nEeUzhOQL_4kNPhq--BtmlM8Ug307CfRz8NsacXPkwp0yHSDxl8mWjrY4BMWxjHQtfgd4Vepx8-Y_2Y555uIJZhGu5QnuJz8qTzY4EXD_WU3FycX7efqvXV5ef2_boKstFT1QDXWymtbbTEJVVohKyFlbio96w2Cux2p0O3Cx0ew2IBIzxnams6ZUHIU3K2-B5y-jZDmdx-KAFH9BHSXBzXqtaNVZoj-uYf9DbNOeJ0jhvDsC3nDVJ8ofA2pWTo3CEPe5-PjjN3H4xbgnEYjLsPxinUvHpwnrd72P1R_E4CAbEABb9iD_mv1v9xfb2IOp-c7_NQ3M0GIYVRa22Mlr8AfP6h1A</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Chaubey, Nidhi</creator><creator>Ghosh, Siddhartha Sankar</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20150201</creationdate><title>Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization</title><author>Chaubey, Nidhi ; Ghosh, Siddhartha Sankar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-9e16b33889633735c9234283155aa0475e8bd6cfdcf0108dcfe72a105b7f58e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>breast neoplasms</topic><topic>cell cycle</topic><topic>Cell Cycle - drug effects</topic><topic>Cell growth</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>cell viability</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>cisplatin</topic><topic>Cisplatin - pharmacology</topic><topic>colony-stimulating factors</topic><topic>cyclins</topic><topic>Cyclins - genetics</topic><topic>Cyclins - metabolism</topic><topic>Cytokines</topic><topic>dose response</topic><topic>doxorubicin</topic><topic>Doxorubicin - pharmacology</topic><topic>Female</topic><topic>Fluorouracil - pharmacology</topic><topic>Gene Expression</topic><topic>gene overexpression</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>macrophages</topic><topic>MCF-7 Cells</topic><topic>neoplasm cells</topic><topic>Plasmids - 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Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaubey, Nidhi</au><au>Ghosh, Siddhartha Sankar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>175</volume><issue>4</issue><spage>1948</spage><epage>1959</epage><pages>1948-1959</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>This report describes the effect of overexpressing granulocyte macrophage colony stimulating factor (GMCSF) in breast cancer cells, which otherwise is involved in proliferation and differentiation of granulocyte and macrophage lineages. The purified recombinant GMCSF cytokine is known to exert dose-dependent proliferative response on various cancer cells, but its effect during overexpression is yet to be evaluated. In our present study, we have generated MCF-7 (breast cancer) cells overexpressing GMCSF. Interestingly, cell viability studies showed pronounced sensitivity of GMCSF overexpressing MCF-7 cells towards anticancer drugs, such as, doxorubicin, 5FU and cisplatin. These findings were substantiated by cell cycle analysis of the drug-treated GMCSF overexpressing MCF-7 cells. Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) results revealed differential expressions of cyclins, and the carboxyfluorescein succinimidyl ester (CFSE)-based assay established decrease in doubling time of GMCSF overexpressed cells with respect to the control populations. Thus, overexpressing of proliferative GMCSF cytokine in breast cancer cells may increase susceptibility to anticancer drugs.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>25432336</pmid><doi>10.1007/s12010-014-1373-5</doi><tpages>12</tpages></addata></record> |
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| subjects | Antineoplastic Agents - pharmacology Biochemistry Biotechnology Breast cancer breast neoplasms cell cycle Cell Cycle - drug effects Cell growth Cell Proliferation - drug effects Cell Survival - drug effects cell viability Chemistry Chemistry and Materials Science cisplatin Cisplatin - pharmacology colony-stimulating factors cyclins Cyclins - genetics Cyclins - metabolism Cytokines dose response doxorubicin Doxorubicin - pharmacology Female Fluorouracil - pharmacology Gene Expression gene overexpression Granulocyte-Macrophage Colony-Stimulating Factor - genetics Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Growth factors Humans Inhibitory Concentration 50 macrophages MCF-7 Cells neoplasm cells Plasmids - chemistry Plasmids - metabolism Protein expression reverse transcriptase polymerase chain reaction Transfection Transgenes |
| title | Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization |
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