A passive micromixer for enzymatic digestion of DNA
•A passive micromixer with zigzag geometry is fabricated using FPC technology.•The total length of the micromixer is estimated by simulation.•The micromixer is evaluated with a DNA digestion process with restriction enzymes.•Simultaneous mixing of enzymes with DNA and DNA digestion is achieved in 2....
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| Veröffentlicht in: | Microelectronic engineering 2014-07, Vol.124, p.42-46 |
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| container_title | Microelectronic engineering |
| container_volume | 124 |
| creator | Papadopoulos, V.E. Kefala, I.N. Kaprou, G. Kokkoris, G. Moschou, D. Papadakis, G. Gizeli, E. Tserepi, A. |
| description | •A passive micromixer with zigzag geometry is fabricated using FPC technology.•The total length of the micromixer is estimated by simulation.•The micromixer is evaluated with a DNA digestion process with restriction enzymes.•Simultaneous mixing of enzymes with DNA and DNA digestion is achieved in 2.5min.
A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37°C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes. |
| doi_str_mv | 10.1016/j.mee.2014.04.011 |
| format | Article |
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A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37°C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes.</description><identifier>ISSN: 0167-9317</identifier><identifier>EISSN: 1873-5568</identifier><identifier>DOI: 10.1016/j.mee.2014.04.011</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Constrictions ; Deoxyribonucleic acid ; Diagnostic systems ; Digestion ; DNA digestion ; Enzymes ; Flexible printed circuit ; Lab on a Chip (LoC) ; Microfluidics ; Passive micromixer ; Platforms ; Printed circuits ; Simulation</subject><ispartof>Microelectronic engineering, 2014-07, Vol.124, p.42-46</ispartof><rights>2014 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-4aac88e6f75902bfae98159ff3f07348245f626f4890659d57e7ebcb3566febb3</citedby><cites>FETCH-LOGICAL-c363t-4aac88e6f75902bfae98159ff3f07348245f626f4890659d57e7ebcb3566febb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mee.2014.04.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids></links><search><creatorcontrib>Papadopoulos, V.E.</creatorcontrib><creatorcontrib>Kefala, I.N.</creatorcontrib><creatorcontrib>Kaprou, G.</creatorcontrib><creatorcontrib>Kokkoris, G.</creatorcontrib><creatorcontrib>Moschou, D.</creatorcontrib><creatorcontrib>Papadakis, G.</creatorcontrib><creatorcontrib>Gizeli, E.</creatorcontrib><creatorcontrib>Tserepi, A.</creatorcontrib><title>A passive micromixer for enzymatic digestion of DNA</title><title>Microelectronic engineering</title><description>•A passive micromixer with zigzag geometry is fabricated using FPC technology.•The total length of the micromixer is estimated by simulation.•The micromixer is evaluated with a DNA digestion process with restriction enzymes.•Simultaneous mixing of enzymes with DNA and DNA digestion is achieved in 2.5min.
A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37°C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes.</description><subject>Constrictions</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>Digestion</subject><subject>DNA digestion</subject><subject>Enzymes</subject><subject>Flexible printed circuit</subject><subject>Lab on a Chip (LoC)</subject><subject>Microfluidics</subject><subject>Passive micromixer</subject><subject>Platforms</subject><subject>Printed circuits</subject><subject>Simulation</subject><issn>0167-9317</issn><issn>1873-5568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkE9LAzEQxYMoWKsfwNseveya2fzZLJ5KtSoUveg5ZLMTSenu1mRbrJ_elHoW4cEw8HvDvEfINdACKMjbVdEhFiUFXtAkgBMyAVWxXAipTskkMVVeM6jOyUWMK5p2TtWEsFm2MTH6HWadt2Ho_BeGzA0hw_5735nR26z1HxhHP_TZ4LL7l9klOXNmHfHqd07J--Lhbf6UL18fn-ezZW6ZZGPOjbFKoXSVqGnZOIO1AlE7xxytGFclF06W0nFVUynqVlRYYWMbJqR02DRsSm6Odzdh-NymF3Tno8X12vQ4bKMGKbismSjZf1DgApiEhMIRTWljDOj0JvjOhL0Gqg9d6pVOXepDl5omwcFzd_RgirvzGHS0HnuLrQ9oR90O_g_3D7olenc</recordid><startdate>20140725</startdate><enddate>20140725</enddate><creator>Papadopoulos, V.E.</creator><creator>Kefala, I.N.</creator><creator>Kaprou, G.</creator><creator>Kokkoris, G.</creator><creator>Moschou, D.</creator><creator>Papadakis, G.</creator><creator>Gizeli, E.</creator><creator>Tserepi, A.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>8FD</scope><scope>L7M</scope><scope>7TM</scope></search><sort><creationdate>20140725</creationdate><title>A passive micromixer for enzymatic digestion of DNA</title><author>Papadopoulos, V.E. ; Kefala, I.N. ; Kaprou, G. ; Kokkoris, G. ; Moschou, D. ; Papadakis, G. ; Gizeli, E. ; Tserepi, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-4aac88e6f75902bfae98159ff3f07348245f626f4890659d57e7ebcb3566febb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Constrictions</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>Digestion</topic><topic>DNA digestion</topic><topic>Enzymes</topic><topic>Flexible printed circuit</topic><topic>Lab on a Chip (LoC)</topic><topic>Microfluidics</topic><topic>Passive micromixer</topic><topic>Platforms</topic><topic>Printed circuits</topic><topic>Simulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papadopoulos, V.E.</creatorcontrib><creatorcontrib>Kefala, I.N.</creatorcontrib><creatorcontrib>Kaprou, G.</creatorcontrib><creatorcontrib>Kokkoris, G.</creatorcontrib><creatorcontrib>Moschou, D.</creatorcontrib><creatorcontrib>Papadakis, G.</creatorcontrib><creatorcontrib>Gizeli, E.</creatorcontrib><creatorcontrib>Tserepi, A.</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Microelectronic engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papadopoulos, V.E.</au><au>Kefala, I.N.</au><au>Kaprou, G.</au><au>Kokkoris, G.</au><au>Moschou, D.</au><au>Papadakis, G.</au><au>Gizeli, E.</au><au>Tserepi, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A passive micromixer for enzymatic digestion of DNA</atitle><jtitle>Microelectronic engineering</jtitle><date>2014-07-25</date><risdate>2014</risdate><volume>124</volume><spage>42</spage><epage>46</epage><pages>42-46</pages><issn>0167-9317</issn><eissn>1873-5568</eissn><abstract>•A passive micromixer with zigzag geometry is fabricated using FPC technology.•The total length of the micromixer is estimated by simulation.•The micromixer is evaluated with a DNA digestion process with restriction enzymes.•Simultaneous mixing of enzymes with DNA and DNA digestion is achieved in 2.5min.
A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37°C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.mee.2014.04.011</doi><tpages>5</tpages></addata></record> |
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| ispartof | Microelectronic engineering, 2014-07, Vol.124, p.42-46 |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Constrictions Deoxyribonucleic acid Diagnostic systems Digestion DNA digestion Enzymes Flexible printed circuit Lab on a Chip (LoC) Microfluidics Passive micromixer Platforms Printed circuits Simulation |
| title | A passive micromixer for enzymatic digestion of DNA |
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