Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation
Background Intragastric or intraperitoneal ethanol (EtOH) treatment inhibits reproductive functions in females and male rats. The area of the hypothalamus where these effects take place is unknown. As the participations of the preoptic‐anterior hypothalamic area (POA‐AHA) in regulating ovulation is...
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| Veröffentlicht in: | Alcoholism, clinical and experimental research clinical and experimental research, 2014-06, Vol.38 (6), p.1611-1621 |
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| description | Background
Intragastric or intraperitoneal ethanol (EtOH) treatment inhibits reproductive functions in females and male rats. The area of the hypothalamus where these effects take place is unknown. As the participations of the preoptic‐anterior hypothalamic area (POA‐AHA) in regulating ovulation is asymmetric, this study aims to analyze the effects on 17β‐estradiol(E2), progesterone, luteinizing hormone (LH), follicle‐stimulating hormone (FSH) serum levels, the messenger ribonucleic acid (mRNA) expression of estrogen receptor alpha (ERα) and beta (ERβ), and ovulation resulting from unilaterally microinjecting water or an EtOH solution into either side of the POA‐AHA.
Methods
The treatment consisted of microinjecting a 8.6 μM EtOH solution into either side of the POA‐AHA. The study was performed on groups of adult cyclic rats at 09.00 hours on diestrus‐1, and sacrificed on diestrus‐2 at 13.00, on proestrus at 09.00 or 17.00 or on estrus at 09.00 hours. Ovulation rates were assessed in rats sacrificed on estrus. Hormonal serum levels were measured using radioimmunoassay, and as a function of ERα and ERβ mRNA expression in each side of the POA‐AHA by reverse transcriptase polymerase chain reaction.
Results
EtOH treatment blocked ovulation and the preovulatory release of LH, and lowered E2 levels. Irrespective of the treated POA‐AHA side, ERα mRNA expression was consistently lower in the left POA‐AHA and higher on the right. EtOH treatment in the left POA‐AHA decreased FSH serum levels and lowered ERβ mRNA expression. In turn, EtOH treatment on the right POA‐AHA resulted in higher FSH levels and ERβ mRNA expression.
Conclusions
The present results show that EtOH blocks the preovulatory surge of LH on the POA‐AHA. The effects of EtOH treatment of preovulatory FSH surge on the POA‐AHA are asymmetric (stimulative on the right and inhibiting in the left). The effects of EtOH treatment on preovulatory LH and FSH surge could be explained by the inhibition of ERα and ERβ mRNA expression, respectively. |
| doi_str_mv | 10.1111/acer.12399 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1654689551</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3962151241</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4289-239b36bf5ffa96f33fc33296afee9d850b8dc571e01a316ad6c1949a5eeb85a13</originalsourceid><addsrcrecordid>eNqNkc1uEzEURi0EoqGw4QGQJTYIaYo9Hv8toyi0QKCoooKd5TjX1GFip_YMkLfHadouWCDuxot7viNdfwg9p-SE1nljHeQT2jKtH6AJ5Yw0pJXyIZoQ2vFGEKKO0JNS1oSQTgnxGB21nWqpaNkEpbn34IaCk8eXMfR2gGz7foc_BpdTiOu6DPE7ng9XNqYeh4iHK8CfM6TtEFwzjTUQUsZnu22qTG83weFpBnujvLB7dcTnP8eqDik-RY-87Qs8u32P0eXb-ZfZWbM4P303my4a17VKN_WYJRNLz723WnjGvGOs1cJ6AL1SnCzVynFJgVDLqLAr4ajutOUAS8UtZcfo1cG7zel6hDKYTSgO-t5GSGMxVPBOKM35f6CcdUQrrlVFX_6FrtOYYz3EUCmklFrSrlKvD1T9wVIyeLPNYWPzzlBi9o2ZfWPmprEKv7hVjssNrO7Ru4oqQA_Ar9DD7h8qM53NL-6kzSETygC_7zM2_zBCMsnN10-nRrxnSqkP38yC_QFnQK_G</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1767779714</pqid></control><display><type>article</type><title>Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>Cruz, María-Esther ; Olvera, Esteban ; Pérez, Marian-Jesabel ; Min, Enrique-Isid ; Flores, Angélica ; Cárdenas, Mario ; Chavira, Roberto ; Domínguez, Roberto</creator><creatorcontrib>Cruz, María-Esther ; Olvera, Esteban ; Pérez, Marian-Jesabel ; Min, Enrique-Isid ; Flores, Angélica ; Cárdenas, Mario ; Chavira, Roberto ; Domínguez, Roberto</creatorcontrib><description>Background
Intragastric or intraperitoneal ethanol (EtOH) treatment inhibits reproductive functions in females and male rats. The area of the hypothalamus where these effects take place is unknown. As the participations of the preoptic‐anterior hypothalamic area (POA‐AHA) in regulating ovulation is asymmetric, this study aims to analyze the effects on 17β‐estradiol(E2), progesterone, luteinizing hormone (LH), follicle‐stimulating hormone (FSH) serum levels, the messenger ribonucleic acid (mRNA) expression of estrogen receptor alpha (ERα) and beta (ERβ), and ovulation resulting from unilaterally microinjecting water or an EtOH solution into either side of the POA‐AHA.
Methods
The treatment consisted of microinjecting a 8.6 μM EtOH solution into either side of the POA‐AHA. The study was performed on groups of adult cyclic rats at 09.00 hours on diestrus‐1, and sacrificed on diestrus‐2 at 13.00, on proestrus at 09.00 or 17.00 or on estrus at 09.00 hours. Ovulation rates were assessed in rats sacrificed on estrus. Hormonal serum levels were measured using radioimmunoassay, and as a function of ERα and ERβ mRNA expression in each side of the POA‐AHA by reverse transcriptase polymerase chain reaction.
Results
EtOH treatment blocked ovulation and the preovulatory release of LH, and lowered E2 levels. Irrespective of the treated POA‐AHA side, ERα mRNA expression was consistently lower in the left POA‐AHA and higher on the right. EtOH treatment in the left POA‐AHA decreased FSH serum levels and lowered ERβ mRNA expression. In turn, EtOH treatment on the right POA‐AHA resulted in higher FSH levels and ERβ mRNA expression.
Conclusions
The present results show that EtOH blocks the preovulatory surge of LH on the POA‐AHA. The effects of EtOH treatment of preovulatory FSH surge on the POA‐AHA are asymmetric (stimulative on the right and inhibiting in the left). The effects of EtOH treatment on preovulatory LH and FSH surge could be explained by the inhibition of ERα and ERβ mRNA expression, respectively.</description><identifier>ISSN: 0145-6008</identifier><identifier>EISSN: 1530-0277</identifier><identifier>DOI: 10.1111/acer.12399</identifier><identifier>PMID: 24821623</identifier><identifier>CODEN: ACRSDM</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; Anterior Hypothalamic Nucleus - drug effects ; Anterior Hypothalamic Nucleus - physiology ; Estradiol - blood ; Estrogen Receptor alpha - biosynthesis ; Estrogen Receptor Alpha mRNA Expression ; Estrogen Receptor beta - biosynthesis ; Estrogen Receptor Beta mRNA Expression ; Ethanol ; Ethanol - administration & dosage ; Ethanol - pharmacology ; Female ; Follicle Stimulating Hormone - blood ; Follicle-Stimulating Hormone ; Follicle‐Stimulating Hormone, Luteinizing Hormone Surge ; Luteinizing Hormone - blood ; Luteinizing Hormone Surge ; Microinjections ; Ovulation - drug effects ; Preoptic Area - drug effects ; Preoptic Area - physiology ; Preoptic-Anterior Hypothalamic Area ; Progesterone - blood ; Rats ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>Alcoholism, clinical and experimental research, 2014-06, Vol.38 (6), p.1611-1621</ispartof><rights>Copyright © 2014 by the Research Society on Alcoholism</rights><rights>Copyright © 2014 by the Research Society on Alcoholism.</rights><rights>2014 Research Society on Alcoholism</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4289-239b36bf5ffa96f33fc33296afee9d850b8dc571e01a316ad6c1949a5eeb85a13</citedby><cites>FETCH-LOGICAL-c4289-239b36bf5ffa96f33fc33296afee9d850b8dc571e01a316ad6c1949a5eeb85a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Facer.12399$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Facer.12399$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24821623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cruz, María-Esther</creatorcontrib><creatorcontrib>Olvera, Esteban</creatorcontrib><creatorcontrib>Pérez, Marian-Jesabel</creatorcontrib><creatorcontrib>Min, Enrique-Isid</creatorcontrib><creatorcontrib>Flores, Angélica</creatorcontrib><creatorcontrib>Cárdenas, Mario</creatorcontrib><creatorcontrib>Chavira, Roberto</creatorcontrib><creatorcontrib>Domínguez, Roberto</creatorcontrib><title>Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation</title><title>Alcoholism, clinical and experimental research</title><addtitle>Alcohol Clin Exp Res</addtitle><description>Background
Intragastric or intraperitoneal ethanol (EtOH) treatment inhibits reproductive functions in females and male rats. The area of the hypothalamus where these effects take place is unknown. As the participations of the preoptic‐anterior hypothalamic area (POA‐AHA) in regulating ovulation is asymmetric, this study aims to analyze the effects on 17β‐estradiol(E2), progesterone, luteinizing hormone (LH), follicle‐stimulating hormone (FSH) serum levels, the messenger ribonucleic acid (mRNA) expression of estrogen receptor alpha (ERα) and beta (ERβ), and ovulation resulting from unilaterally microinjecting water or an EtOH solution into either side of the POA‐AHA.
Methods
The treatment consisted of microinjecting a 8.6 μM EtOH solution into either side of the POA‐AHA. The study was performed on groups of adult cyclic rats at 09.00 hours on diestrus‐1, and sacrificed on diestrus‐2 at 13.00, on proestrus at 09.00 or 17.00 or on estrus at 09.00 hours. Ovulation rates were assessed in rats sacrificed on estrus. Hormonal serum levels were measured using radioimmunoassay, and as a function of ERα and ERβ mRNA expression in each side of the POA‐AHA by reverse transcriptase polymerase chain reaction.
Results
EtOH treatment blocked ovulation and the preovulatory release of LH, and lowered E2 levels. Irrespective of the treated POA‐AHA side, ERα mRNA expression was consistently lower in the left POA‐AHA and higher on the right. EtOH treatment in the left POA‐AHA decreased FSH serum levels and lowered ERβ mRNA expression. In turn, EtOH treatment on the right POA‐AHA resulted in higher FSH levels and ERβ mRNA expression.
Conclusions
The present results show that EtOH blocks the preovulatory surge of LH on the POA‐AHA. The effects of EtOH treatment of preovulatory FSH surge on the POA‐AHA are asymmetric (stimulative on the right and inhibiting in the left). The effects of EtOH treatment on preovulatory LH and FSH surge could be explained by the inhibition of ERα and ERβ mRNA expression, respectively.</description><subject>Animals</subject><subject>Anterior Hypothalamic Nucleus - drug effects</subject><subject>Anterior Hypothalamic Nucleus - physiology</subject><subject>Estradiol - blood</subject><subject>Estrogen Receptor alpha - biosynthesis</subject><subject>Estrogen Receptor Alpha mRNA Expression</subject><subject>Estrogen Receptor beta - biosynthesis</subject><subject>Estrogen Receptor Beta mRNA Expression</subject><subject>Ethanol</subject><subject>Ethanol - administration & dosage</subject><subject>Ethanol - pharmacology</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Follicle-Stimulating Hormone</subject><subject>Follicle‐Stimulating Hormone, Luteinizing Hormone Surge</subject><subject>Luteinizing Hormone - blood</subject><subject>Luteinizing Hormone Surge</subject><subject>Microinjections</subject><subject>Ovulation - drug effects</subject><subject>Preoptic Area - drug effects</subject><subject>Preoptic Area - physiology</subject><subject>Preoptic-Anterior Hypothalamic Area</subject><subject>Progesterone - blood</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>0145-6008</issn><issn>1530-0277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEURi0EoqGw4QGQJTYIaYo9Hv8toyi0QKCoooKd5TjX1GFip_YMkLfHadouWCDuxot7viNdfwg9p-SE1nljHeQT2jKtH6AJ5Yw0pJXyIZoQ2vFGEKKO0JNS1oSQTgnxGB21nWqpaNkEpbn34IaCk8eXMfR2gGz7foc_BpdTiOu6DPE7ng9XNqYeh4iHK8CfM6TtEFwzjTUQUsZnu22qTG83weFpBnujvLB7dcTnP8eqDik-RY-87Qs8u32P0eXb-ZfZWbM4P303my4a17VKN_WYJRNLz723WnjGvGOs1cJ6AL1SnCzVynFJgVDLqLAr4ajutOUAS8UtZcfo1cG7zel6hDKYTSgO-t5GSGMxVPBOKM35f6CcdUQrrlVFX_6FrtOYYz3EUCmklFrSrlKvD1T9wVIyeLPNYWPzzlBi9o2ZfWPmprEKv7hVjssNrO7Ru4oqQA_Ar9DD7h8qM53NL-6kzSETygC_7zM2_zBCMsnN10-nRrxnSqkP38yC_QFnQK_G</recordid><startdate>201406</startdate><enddate>201406</enddate><creator>Cruz, María-Esther</creator><creator>Olvera, Esteban</creator><creator>Pérez, Marian-Jesabel</creator><creator>Min, Enrique-Isid</creator><creator>Flores, Angélica</creator><creator>Cárdenas, Mario</creator><creator>Chavira, Roberto</creator><creator>Domínguez, Roberto</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>K7.</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201406</creationdate><title>Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation</title><author>Cruz, María-Esther ; Olvera, Esteban ; Pérez, Marian-Jesabel ; Min, Enrique-Isid ; Flores, Angélica ; Cárdenas, Mario ; Chavira, Roberto ; Domínguez, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4289-239b36bf5ffa96f33fc33296afee9d850b8dc571e01a316ad6c1949a5eeb85a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anterior Hypothalamic Nucleus - drug effects</topic><topic>Anterior Hypothalamic Nucleus - physiology</topic><topic>Estradiol - blood</topic><topic>Estrogen Receptor alpha - biosynthesis</topic><topic>Estrogen Receptor Alpha mRNA Expression</topic><topic>Estrogen Receptor beta - biosynthesis</topic><topic>Estrogen Receptor Beta mRNA Expression</topic><topic>Ethanol</topic><topic>Ethanol - administration & dosage</topic><topic>Ethanol - pharmacology</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Follicle-Stimulating Hormone</topic><topic>Follicle‐Stimulating Hormone, Luteinizing Hormone Surge</topic><topic>Luteinizing Hormone - blood</topic><topic>Luteinizing Hormone Surge</topic><topic>Microinjections</topic><topic>Ovulation - drug effects</topic><topic>Preoptic Area - drug effects</topic><topic>Preoptic Area - physiology</topic><topic>Preoptic-Anterior Hypothalamic Area</topic><topic>Progesterone - blood</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cruz, María-Esther</creatorcontrib><creatorcontrib>Olvera, Esteban</creatorcontrib><creatorcontrib>Pérez, Marian-Jesabel</creatorcontrib><creatorcontrib>Min, Enrique-Isid</creatorcontrib><creatorcontrib>Flores, Angélica</creatorcontrib><creatorcontrib>Cárdenas, Mario</creatorcontrib><creatorcontrib>Chavira, Roberto</creatorcontrib><creatorcontrib>Domínguez, Roberto</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Alcoholism, clinical and experimental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cruz, María-Esther</au><au>Olvera, Esteban</au><au>Pérez, Marian-Jesabel</au><au>Min, Enrique-Isid</au><au>Flores, Angélica</au><au>Cárdenas, Mario</au><au>Chavira, Roberto</au><au>Domínguez, Roberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation</atitle><jtitle>Alcoholism, clinical and experimental research</jtitle><addtitle>Alcohol Clin Exp Res</addtitle><date>2014-06</date><risdate>2014</risdate><volume>38</volume><issue>6</issue><spage>1611</spage><epage>1621</epage><pages>1611-1621</pages><issn>0145-6008</issn><eissn>1530-0277</eissn><coden>ACRSDM</coden><abstract>Background
Intragastric or intraperitoneal ethanol (EtOH) treatment inhibits reproductive functions in females and male rats. The area of the hypothalamus where these effects take place is unknown. As the participations of the preoptic‐anterior hypothalamic area (POA‐AHA) in regulating ovulation is asymmetric, this study aims to analyze the effects on 17β‐estradiol(E2), progesterone, luteinizing hormone (LH), follicle‐stimulating hormone (FSH) serum levels, the messenger ribonucleic acid (mRNA) expression of estrogen receptor alpha (ERα) and beta (ERβ), and ovulation resulting from unilaterally microinjecting water or an EtOH solution into either side of the POA‐AHA.
Methods
The treatment consisted of microinjecting a 8.6 μM EtOH solution into either side of the POA‐AHA. The study was performed on groups of adult cyclic rats at 09.00 hours on diestrus‐1, and sacrificed on diestrus‐2 at 13.00, on proestrus at 09.00 or 17.00 or on estrus at 09.00 hours. Ovulation rates were assessed in rats sacrificed on estrus. Hormonal serum levels were measured using radioimmunoassay, and as a function of ERα and ERβ mRNA expression in each side of the POA‐AHA by reverse transcriptase polymerase chain reaction.
Results
EtOH treatment blocked ovulation and the preovulatory release of LH, and lowered E2 levels. Irrespective of the treated POA‐AHA side, ERα mRNA expression was consistently lower in the left POA‐AHA and higher on the right. EtOH treatment in the left POA‐AHA decreased FSH serum levels and lowered ERβ mRNA expression. In turn, EtOH treatment on the right POA‐AHA resulted in higher FSH levels and ERβ mRNA expression.
Conclusions
The present results show that EtOH blocks the preovulatory surge of LH on the POA‐AHA. The effects of EtOH treatment of preovulatory FSH surge on the POA‐AHA are asymmetric (stimulative on the right and inhibiting in the left). The effects of EtOH treatment on preovulatory LH and FSH surge could be explained by the inhibition of ERα and ERβ mRNA expression, respectively.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24821623</pmid><doi>10.1111/acer.12399</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-6008 |
| ispartof | Alcoholism, clinical and experimental research, 2014-06, Vol.38 (6), p.1611-1621 |
| issn | 0145-6008 1530-0277 |
| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Journals@Ovid Complete |
| subjects | Animals Anterior Hypothalamic Nucleus - drug effects Anterior Hypothalamic Nucleus - physiology Estradiol - blood Estrogen Receptor alpha - biosynthesis Estrogen Receptor Alpha mRNA Expression Estrogen Receptor beta - biosynthesis Estrogen Receptor Beta mRNA Expression Ethanol Ethanol - administration & dosage Ethanol - pharmacology Female Follicle Stimulating Hormone - blood Follicle-Stimulating Hormone Follicle‐Stimulating Hormone, Luteinizing Hormone Surge Luteinizing Hormone - blood Luteinizing Hormone Surge Microinjections Ovulation - drug effects Preoptic Area - drug effects Preoptic Area - physiology Preoptic-Anterior Hypothalamic Area Progesterone - blood Rats Reverse Transcriptase Polymerase Chain Reaction |
| title | Effects of Unilaterally Microinjecting Ethanol in the Preoptic-Anterior Hypothalamic Areas of Rats on Ovulation |
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