Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells
•Tissue engineering approach combining MSCs, simvastatin and a 3D porous scaffold.•Induction of osteogenic differentiation in MSCs by simvastatin coated scaffolds.•Lower concentration of simvastatin needed in 3D culture than reported for 2D culture.•A strategy for human adipose tissue-derived MSC ba...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2014-04, Vol.447 (1), p.139-144 |
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| creator | Pullisaar, Helen Reseland, Janne E. Haugen, Håvard J. Brinchmann, Jan E. Østrup, Esben |
| description | •Tissue engineering approach combining MSCs, simvastatin and a 3D porous scaffold.•Induction of osteogenic differentiation in MSCs by simvastatin coated scaffolds.•Lower concentration of simvastatin needed in 3D culture than reported for 2D culture.•A strategy for human adipose tissue-derived MSC based bone tissue engineering.
Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering. |
| doi_str_mv | 10.1016/j.bbrc.2014.03.133 |
| format | Article |
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Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.03.133</identifier><identifier>PMID: 24704451</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipose Tissue - cytology ; Adult ; Alginate hydrogel ; Cell Differentiation - drug effects ; Cell Survival ; Female ; Human adipose tissue-derived mesenchymal stem cells ; Humans ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - physiology ; Middle Aged ; Osteocalcin - secretion ; Osteogenesis ; Osteopontin - secretion ; Osteoprotegerin - secretion ; RNA, Messenger - metabolism ; Simvastatin ; Simvastatin - pharmacology ; TiO2 scaffold ; Tissue Engineering - methods ; Tissue Scaffolds ; Titanium ; Vascular Endothelial Growth Factor A - secretion</subject><ispartof>Biochemical and biophysical research communications, 2014-04, Vol.447 (1), p.139-144</ispartof><rights>2014 The Authors</rights><rights>Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-c152abba35a645365a78908b42d29880021bab3ead3a7d638a12a228c9dd27693</citedby><cites>FETCH-LOGICAL-c433t-c152abba35a645365a78908b42d29880021bab3ead3a7d638a12a228c9dd27693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2014.03.133$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24704451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pullisaar, Helen</creatorcontrib><creatorcontrib>Reseland, Janne E.</creatorcontrib><creatorcontrib>Haugen, Håvard J.</creatorcontrib><creatorcontrib>Brinchmann, Jan E.</creatorcontrib><creatorcontrib>Østrup, Esben</creatorcontrib><title>Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•Tissue engineering approach combining MSCs, simvastatin and a 3D porous scaffold.•Induction of osteogenic differentiation in MSCs by simvastatin coated scaffolds.•Lower concentration of simvastatin needed in 3D culture than reported for 2D culture.•A strategy for human adipose tissue-derived MSC based bone tissue engineering.
Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering.</description><subject>Adipose Tissue - cytology</subject><subject>Adult</subject><subject>Alginate hydrogel</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Survival</subject><subject>Female</subject><subject>Human adipose tissue-derived mesenchymal stem cells</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Middle Aged</subject><subject>Osteocalcin - secretion</subject><subject>Osteogenesis</subject><subject>Osteopontin - secretion</subject><subject>Osteoprotegerin - secretion</subject><subject>RNA, Messenger - metabolism</subject><subject>Simvastatin</subject><subject>Simvastatin - pharmacology</subject><subject>TiO2 scaffold</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><subject>Titanium</subject><subject>Vascular Endothelial Growth Factor A - secretion</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAURS1ERYfCH2CBvGST4K84tsQGVbQgVeqiRWJnvdgvrUdJPNjJSN3w20k0hSWs7ubcq6d3CHnHWc0Z1x_3dddlXwvGVc1kzaV8QXacWVYJztRLsmOM6UpY_uOcvC5lzxjnSttX5FyolinV8B35dRfHI5QZ5jhRn7Z4oKmn9_FW0OKh79MQaJzC4rHQVGZMDzhFT0Pse8w4zXHtpGnrPC4jTBRCPKSCdI6lLFgFzPGIgY5YcPKPTyMMdF0ZqcdhKG_IWQ9DwbfPeUG-X325v_xa3dxef7v8fFN5JeVced4I6DqQDWjVSN1AaywznRJBWGMYE7yDTiIECW3Q0gAXIITxNgTRaisvyIfT7iGnnwuW2Y2xbBfAhGkpjutGaWOMbf6PNtwaaVulV1ScUJ9TKRl7d8hxhPzkOHObIrd3myK3KXJMulXRWnr_vL90I4a_lT9OVuDTCcD1IceI2RUf199hiBn97EKK_9r_DTDyo88</recordid><startdate>20140425</startdate><enddate>20140425</enddate><creator>Pullisaar, Helen</creator><creator>Reseland, Janne E.</creator><creator>Haugen, Håvard J.</creator><creator>Brinchmann, Jan E.</creator><creator>Østrup, Esben</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20140425</creationdate><title>Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells</title><author>Pullisaar, Helen ; Reseland, Janne E. ; Haugen, Håvard J. ; Brinchmann, Jan E. ; Østrup, Esben</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-c152abba35a645365a78908b42d29880021bab3ead3a7d638a12a228c9dd27693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adult</topic><topic>Alginate hydrogel</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Survival</topic><topic>Female</topic><topic>Human adipose tissue-derived mesenchymal stem cells</topic><topic>Humans</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Middle Aged</topic><topic>Osteocalcin - secretion</topic><topic>Osteogenesis</topic><topic>Osteopontin - secretion</topic><topic>Osteoprotegerin - secretion</topic><topic>RNA, Messenger - metabolism</topic><topic>Simvastatin</topic><topic>Simvastatin - pharmacology</topic><topic>TiO2 scaffold</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><topic>Titanium</topic><topic>Vascular Endothelial Growth Factor A - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pullisaar, Helen</creatorcontrib><creatorcontrib>Reseland, Janne E.</creatorcontrib><creatorcontrib>Haugen, Håvard J.</creatorcontrib><creatorcontrib>Brinchmann, Jan E.</creatorcontrib><creatorcontrib>Østrup, Esben</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pullisaar, Helen</au><au>Reseland, Janne E.</au><au>Haugen, Håvard J.</au><au>Brinchmann, Jan E.</au><au>Østrup, Esben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-04-25</date><risdate>2014</risdate><volume>447</volume><issue>1</issue><spage>139</spage><epage>144</epage><pages>139-144</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•Tissue engineering approach combining MSCs, simvastatin and a 3D porous scaffold.•Induction of osteogenic differentiation in MSCs by simvastatin coated scaffolds.•Lower concentration of simvastatin needed in 3D culture than reported for 2D culture.•A strategy for human adipose tissue-derived MSC based bone tissue engineering.
Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24704451</pmid><doi>10.1016/j.bbrc.2014.03.133</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose Tissue - cytology Adult Alginate hydrogel Cell Differentiation - drug effects Cell Survival Female Human adipose tissue-derived mesenchymal stem cells Humans Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Middle Aged Osteocalcin - secretion Osteogenesis Osteopontin - secretion Osteoprotegerin - secretion RNA, Messenger - metabolism Simvastatin Simvastatin - pharmacology TiO2 scaffold Tissue Engineering - methods Tissue Scaffolds Titanium Vascular Endothelial Growth Factor A - secretion |
| title | Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells |
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