Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold
Stem cells from human exfoliated deciduous teeth (SHEDs) have been considered as alternative sources of adult stem cells in tissue engineering because of their potential to differentiate into multiple cell lineages. Strontium has an important function in bone remodeling because it can simulate bone...
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| Veröffentlicht in: | Materials Science & Engineering C 2014-08, Vol.41, p.152-160 |
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| creator | Su, Wen-Ta Wu, Pai-Shuen Ko, Chih-Sheng Huang, Te-Yang |
| description | Stem cells from human exfoliated deciduous teeth (SHEDs) have been considered as alternative sources of adult stem cells in tissue engineering because of their potential to differentiate into multiple cell lineages. Strontium has an important function in bone remodeling because it can simulate bone formation and decrease bone resorption. In this study, the effects of strontium phosphate on the osteogenic differentiation of SHEDs were investigated. Strontium phosphate was found to enhance the osteogenic differentiation of SHEDs with up-regulated osteoblast-related gene expression. The proliferation of SHEDs was slightly inhibited by chitosan scaffolds; however, type-I collagen expression, alkaline phosphatase activity, and calcium deposition on chitosan scaffolds containing strontium were significantly enhanced. Furthermore, cells seeded in a 3D scaffold under dynamic culture at an optimal fluid rate might enhance cellular differentiation than static culture in osteoblastic gene expression. This experiment might provide a useful cell resource and dynamic 3D culture for tissue engineering and bone repair.
•SHEDs have been considered as alternative sources of adult stem cells in tissue engineering•Strontium phosphate can enhance the osteogenic differentiation of SHEDs•3D scaffold under dynamic culture with optimal fluid rate enhance cellular differentiation |
| doi_str_mv | 10.1016/j.msec.2014.04.048 |
| format | Article |
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•SHEDs have been considered as alternative sources of adult stem cells in tissue engineering•Strontium phosphate can enhance the osteogenic differentiation of SHEDs•3D scaffold under dynamic culture with optimal fluid rate enhance cellular differentiation</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bones</subject><subject>Calcium - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan scaffold</subject><subject>Collagen Type I - metabolism</subject><subject>Differentiation</subject><subject>Dynamic culture</subject><subject>Human exfoliated deciduous teeth stem cells (SHEDs)</subject><subject>Humans</subject><subject>Osteogenesis - drug effects</subject><subject>Osteogenic differentiation</subject><subject>Phosphates - chemistry</subject><subject>Phosphates - pharmacology</subject><subject>Scaffolds</subject><subject>Sheds</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Strontium</subject><subject>Strontium - chemistry</subject><subject>Strontium - pharmacology</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><subject>Tooth, Deciduous - cytology</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9rFTEQxYMo9rb6BXyQPPqyt_m3SRZ8kVKtUOiLPofcZOLNZXdTk2yp_fRmudXHtjDDwPA7h2EOQh8o2VJC5flhOxVwW0ao2JK19Cu0oVrxjtCBvkYbMjDdiYHTE3RayoEQqblib9EJEwNRSugNergpFdIvmKPDPoYAGeYabY1pxnb2eIozZDvGh-MqBbxfJjtjuA9pbBx47MFFv6Sl4ApQ97gZTtjBOBbcFFNqtrFhbh9rKk1anA1N7N-hN8GOBd4_zjP08-vlj4ur7vrm2_eLL9ed6wmvnWYDAWFd4E7K3U5SAmEXQA6DtsIRyjnTnFMvg5DEK95TEVgvCGjtlVIDP0Ofjr63Of1eoFQzxbLeZ2doVxsqe9Eew2j_AlQwJmjr59GeC6G4kqqh7Ii6nErJEMxtjpPNfwwlZk3SHMyapFmTNGQt3UQfH_2X3QT-v-RfdA34fASg_e4uQjbFRZgd-JjBVeNTfMr_L5OssE8</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Su, Wen-Ta</creator><creator>Wu, Pai-Shuen</creator><creator>Ko, Chih-Sheng</creator><creator>Huang, Te-Yang</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7QO</scope><scope>P64</scope></search><sort><creationdate>20140801</creationdate><title>Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold</title><author>Su, Wen-Ta ; Wu, Pai-Shuen ; Ko, Chih-Sheng ; Huang, Te-Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-8290e4acf3c66bb610efbfe6998a4c013328331d6f460d73514f2540e88d77793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bones</topic><topic>Calcium - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan scaffold</topic><topic>Collagen Type I - metabolism</topic><topic>Differentiation</topic><topic>Dynamic culture</topic><topic>Human exfoliated deciduous teeth stem cells (SHEDs)</topic><topic>Humans</topic><topic>Osteogenesis - drug effects</topic><topic>Osteogenic differentiation</topic><topic>Phosphates - chemistry</topic><topic>Phosphates - pharmacology</topic><topic>Scaffolds</topic><topic>Sheds</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Strontium</topic><topic>Strontium - chemistry</topic><topic>Strontium - pharmacology</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds</topic><topic>Tooth, Deciduous - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>Su, Wen-Ta</creatorcontrib><creatorcontrib>Wu, Pai-Shuen</creatorcontrib><creatorcontrib>Ko, Chih-Sheng</creatorcontrib><creatorcontrib>Huang, Te-Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Materials Science & Engineering C</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Wen-Ta</au><au>Wu, Pai-Shuen</au><au>Ko, Chih-Sheng</au><au>Huang, Te-Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>41</volume><spage>152</spage><epage>160</epage><pages>152-160</pages><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>Stem cells from human exfoliated deciduous teeth (SHEDs) have been considered as alternative sources of adult stem cells in tissue engineering because of their potential to differentiate into multiple cell lineages. Strontium has an important function in bone remodeling because it can simulate bone formation and decrease bone resorption. In this study, the effects of strontium phosphate on the osteogenic differentiation of SHEDs were investigated. Strontium phosphate was found to enhance the osteogenic differentiation of SHEDs with up-regulated osteoblast-related gene expression. The proliferation of SHEDs was slightly inhibited by chitosan scaffolds; however, type-I collagen expression, alkaline phosphatase activity, and calcium deposition on chitosan scaffolds containing strontium were significantly enhanced. Furthermore, cells seeded in a 3D scaffold under dynamic culture at an optimal fluid rate might enhance cellular differentiation than static culture in osteoblastic gene expression. This experiment might provide a useful cell resource and dynamic 3D culture for tissue engineering and bone repair.
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| subjects | Alkaline Phosphatase - metabolism Biocompatibility Biomedical materials Bones Calcium - metabolism Cell Differentiation - drug effects Cells, Cultured Chitosan Chitosan - chemistry Chitosan scaffold Collagen Type I - metabolism Differentiation Dynamic culture Human exfoliated deciduous teeth stem cells (SHEDs) Humans Osteogenesis - drug effects Osteogenic differentiation Phosphates - chemistry Phosphates - pharmacology Scaffolds Sheds Stem Cells - cytology Stem Cells - metabolism Strontium Strontium - chemistry Strontium - pharmacology Tissue Engineering Tissue Scaffolds Tooth, Deciduous - cytology |
| title | Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold |
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