Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865
Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neut...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-10, Vol.9 (10) |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 10 |
| container_start_page | |
| container_title | PloS one |
| container_volume | 9 |
| creator | Cohen, Noah D Bourquin, Jessica R Bordin, Angela I Kuskie, Kyle R Brake, Courtney N Weaver, Kaytee B Liu, Mei Felippe, M JuliaB Kogut, Michael H |
| description | Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon- gamma (IFN- gamma ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of beta -D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P |
| doi_str_mv | 10.1371/journal.pone.0109865 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1654681031</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1654681031</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_16546810313</originalsourceid><addsrcrecordid>eNqVjs9KAzEYxIMgWP-8QQ85etk1abpx602Kqz1UQb2XkP1qU7L51nwJ2IfwnY3YF_A0zPCbYRibSlFLdStv9phjML4eMUAtpFi0ujlhE7lQs0rPhDpj50R7IRrVaj1h36uQohky2exN5Pf94IKjEiWHgeOWG_52CGkHyVm-HB-rF-8-sAf8OoRsPWByPfA19qWegHiXg_2tGs9fgcoJKmGZeYacIo4754-2IKlAHRpPdxz-jl6y023xcHXUC3bdPbwvn6ox4mcGSpvBkQXvTQDMtJG6metWCiXVP9AfML5hGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1654681031</pqid></control><display><type>article</type><title>Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Cohen, Noah D ; Bourquin, Jessica R ; Bordin, Angela I ; Kuskie, Kyle R ; Brake, Courtney N ; Weaver, Kaytee B ; Liu, Mei ; Felippe, M JuliaB ; Kogut, Michael H</creator><creatorcontrib>Cohen, Noah D ; Bourquin, Jessica R ; Bordin, Angela I ; Kuskie, Kyle R ; Brake, Courtney N ; Weaver, Kaytee B ; Liu, Mei ; Felippe, M JuliaB ; Kogut, Michael H</creatorcontrib><description>Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon- gamma (IFN- gamma ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of beta -D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P<0.05) increased IFN- gamma mRNA expression by foal neutrophils on days 3, 9, and 14. Degranulation was significantly (P<0.05) lower for foals in the CpG-ODN-treated group than the control group at days 3 and 14, but not at other days. No effect of treatment on ROS generation was detected. These results indicate that CpG-ODN administration to foals might improve innate and adaptive immune responses that could protect foals against infectious diseases and possibly improve responses to vaccination.</description><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0109865</identifier><language>eng</language><subject>Rhodococcus</subject><ispartof>PloS one, 2014-10, Vol.9 (10)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Cohen, Noah D</creatorcontrib><creatorcontrib>Bourquin, Jessica R</creatorcontrib><creatorcontrib>Bordin, Angela I</creatorcontrib><creatorcontrib>Kuskie, Kyle R</creatorcontrib><creatorcontrib>Brake, Courtney N</creatorcontrib><creatorcontrib>Weaver, Kaytee B</creatorcontrib><creatorcontrib>Liu, Mei</creatorcontrib><creatorcontrib>Felippe, M JuliaB</creatorcontrib><creatorcontrib>Kogut, Michael H</creatorcontrib><title>Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865</title><title>PloS one</title><description>Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon- gamma (IFN- gamma ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of beta -D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P<0.05) increased IFN- gamma mRNA expression by foal neutrophils on days 3, 9, and 14. Degranulation was significantly (P<0.05) lower for foals in the CpG-ODN-treated group than the control group at days 3 and 14, but not at other days. No effect of treatment on ROS generation was detected. These results indicate that CpG-ODN administration to foals might improve innate and adaptive immune responses that could protect foals against infectious diseases and possibly improve responses to vaccination.</description><subject>Rhodococcus</subject><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqVjs9KAzEYxIMgWP-8QQ85etk1abpx602Kqz1UQb2XkP1qU7L51nwJ2IfwnY3YF_A0zPCbYRibSlFLdStv9phjML4eMUAtpFi0ujlhE7lQs0rPhDpj50R7IRrVaj1h36uQohky2exN5Pf94IKjEiWHgeOWG_52CGkHyVm-HB-rF-8-sAf8OoRsPWByPfA19qWegHiXg_2tGs9fgcoJKmGZeYacIo4754-2IKlAHRpPdxz-jl6y023xcHXUC3bdPbwvn6ox4mcGSpvBkQXvTQDMtJG6metWCiXVP9AfML5hGg</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Cohen, Noah D</creator><creator>Bourquin, Jessica R</creator><creator>Bordin, Angela I</creator><creator>Kuskie, Kyle R</creator><creator>Brake, Courtney N</creator><creator>Weaver, Kaytee B</creator><creator>Liu, Mei</creator><creator>Felippe, M JuliaB</creator><creator>Kogut, Michael H</creator><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20141001</creationdate><title>Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865</title><author>Cohen, Noah D ; Bourquin, Jessica R ; Bordin, Angela I ; Kuskie, Kyle R ; Brake, Courtney N ; Weaver, Kaytee B ; Liu, Mei ; Felippe, M JuliaB ; Kogut, Michael H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_16546810313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Rhodococcus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cohen, Noah D</creatorcontrib><creatorcontrib>Bourquin, Jessica R</creatorcontrib><creatorcontrib>Bordin, Angela I</creatorcontrib><creatorcontrib>Kuskie, Kyle R</creatorcontrib><creatorcontrib>Brake, Courtney N</creatorcontrib><creatorcontrib>Weaver, Kaytee B</creatorcontrib><creatorcontrib>Liu, Mei</creatorcontrib><creatorcontrib>Felippe, M JuliaB</creatorcontrib><creatorcontrib>Kogut, Michael H</creatorcontrib><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cohen, Noah D</au><au>Bourquin, Jessica R</au><au>Bordin, Angela I</au><au>Kuskie, Kyle R</au><au>Brake, Courtney N</au><au>Weaver, Kaytee B</au><au>Liu, Mei</au><au>Felippe, M JuliaB</au><au>Kogut, Michael H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865</atitle><jtitle>PloS one</jtitle><date>2014-10-01</date><risdate>2014</risdate><volume>9</volume><issue>10</issue><eissn>1932-6203</eissn><abstract>Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon- gamma (IFN- gamma ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of beta -D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P<0.05) increased IFN- gamma mRNA expression by foal neutrophils on days 3, 9, and 14. Degranulation was significantly (P<0.05) lower for foals in the CpG-ODN-treated group than the control group at days 3 and 14, but not at other days. No effect of treatment on ROS generation was detected. These results indicate that CpG-ODN administration to foals might improve innate and adaptive immune responses that could protect foals against infectious diseases and possibly improve responses to vaccination.</abstract><doi>10.1371/journal.pone.0109865</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1932-6203 |
| ispartof | PloS one, 2014-10, Vol.9 (10) |
| issn | 1932-6203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1654681031 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Rhodococcus |
| title | Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals: e109865 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T01%3A01%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intramuscular%20Administration%20of%20a%20Synthetic%20CpG-Oligodeoxynucleotide%20Modulates%20Functional%20Responses%20of%20Neutrophils%20of%20Neonatal%20Foals:%20e109865&rft.jtitle=PloS%20one&rft.au=Cohen,%20Noah%20D&rft.date=2014-10-01&rft.volume=9&rft.issue=10&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0109865&rft_dat=%3Cproquest%3E1654681031%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1654681031&rft_id=info:pmid/&rfr_iscdi=true |