Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9
•Adenoviral delivery of CRISPR/Cas9 produces efficient gene editing in cultured cells.•Effective gene knockout in the adult mouse liver via adenoviral delivery of CRISPR/Cas9.•The gene editing in the liver is stable over long term and after extensive liver tissue regeneration. We developed an adenov...
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| Veröffentlicht in: | FEBS letters 2014-11, Vol.588 (21), p.3954-3958 |
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| container_title | FEBS letters |
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| creator | Cheng, Ranran Peng, Jin Yan, Yonghong Cao, Peili Wang, Jiewei Qiu, Chen Tang, Lichun Liu, Di Tang, Li Jin, Jianping Huang, Xingxu He, Fuchu Zhang, Pumin |
| description | •Adenoviral delivery of CRISPR/Cas9 produces efficient gene editing in cultured cells.•Effective gene knockout in the adult mouse liver via adenoviral delivery of CRISPR/Cas9.•The gene editing in the liver is stable over long term and after extensive liver tissue regeneration.
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings. |
| doi_str_mv | 10.1016/j.febslet.2014.09.008 |
| format | Article |
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We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.09.008</identifier><identifier>PMID: 25241167</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adenoviridae - genetics ; Adenoviruses ; Animals ; Base Sequence ; Clustered Regularly Interspaced Short Palindromic Repeats - genetics ; CRISPR/Cas9 ; Gene editing ; Gene Targeting - methods ; Humans ; Liver ; Liver - metabolism ; Male ; Mice</subject><ispartof>FEBS letters, 2014-11, Vol.588 (21), p.3954-3958</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5628-8f76c695ebd401c594c8104ed662dbd30dbf640b8f1a922ec14056d4b906447c3</citedby><cites>FETCH-LOGICAL-c5628-8f76c695ebd401c594c8104ed662dbd30dbf640b8f1a922ec14056d4b906447c3</cites><orcidid>0000-0002-1846-938X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.09.008$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579314006838$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25241167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Ranran</creatorcontrib><creatorcontrib>Peng, Jin</creatorcontrib><creatorcontrib>Yan, Yonghong</creatorcontrib><creatorcontrib>Cao, Peili</creatorcontrib><creatorcontrib>Wang, Jiewei</creatorcontrib><creatorcontrib>Qiu, Chen</creatorcontrib><creatorcontrib>Tang, Lichun</creatorcontrib><creatorcontrib>Liu, Di</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><creatorcontrib>Jin, Jianping</creatorcontrib><creatorcontrib>Huang, Xingxu</creatorcontrib><creatorcontrib>He, Fuchu</creatorcontrib><creatorcontrib>Zhang, Pumin</creatorcontrib><title>Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Adenoviral delivery of CRISPR/Cas9 produces efficient gene editing in cultured cells.•Effective gene knockout in the adult mouse liver via adenoviral delivery of CRISPR/Cas9.•The gene editing in the liver is stable over long term and after extensive liver tissue regeneration.
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.</description><subject>Adenoviridae - genetics</subject><subject>Adenoviruses</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Clustered Regularly Interspaced Short Palindromic Repeats - genetics</subject><subject>CRISPR/Cas9</subject><subject>Gene editing</subject><subject>Gene Targeting - methods</subject><subject>Humans</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vGyEQhlHVqnHS_oRWHHvZDbDAwilKLOdDipQqbs9oF4YIa72bwNqR_33Z2sk1PiGGZ94Z8SD0g5KSEirPV6WHNnUwloxQXhJdEqI-oRlVdVVUXKrPaEbySyFqXZ2g05RWJN8V1V_RCROMUyrrGVouvA82QD_iJ-gBgwtj6J9w6HHjNt2I18MmAe7CFmLC29DkMvTDNsSmww7-13d48Hj-eLf8_Xg-b5L-hr74pkvw_XCeob_Xiz_z2-L-4eZufnlfWCGZKpSvpZVaQOs4oVZobhUlHJyUzLWuIq71kpNWedpoxsBSToR0vNVEcl7b6gz92uc-x-FlA2k065AsdF3TQ97aUCm4rGvO5HGo1IyqI1CmtRC1EBkVe9TGIaUI3jzHsG7izlBiJktmZQ6WzGTJEG2ypdz38zBi067BvXe9acnA7R54DR3sjks114srtpyUT8bzXxGpqmnWxT4KsoltgGjSpNtm0RHsaNwQPtj2H4hGuRk</recordid><startdate>20141103</startdate><enddate>20141103</enddate><creator>Cheng, Ranran</creator><creator>Peng, Jin</creator><creator>Yan, Yonghong</creator><creator>Cao, Peili</creator><creator>Wang, Jiewei</creator><creator>Qiu, Chen</creator><creator>Tang, Lichun</creator><creator>Liu, Di</creator><creator>Tang, Li</creator><creator>Jin, Jianping</creator><creator>Huang, Xingxu</creator><creator>He, Fuchu</creator><creator>Zhang, Pumin</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-1846-938X</orcidid></search><sort><creationdate>20141103</creationdate><title>Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9</title><author>Cheng, Ranran ; Peng, Jin ; Yan, Yonghong ; Cao, Peili ; Wang, Jiewei ; Qiu, Chen ; Tang, Lichun ; Liu, Di ; Tang, Li ; Jin, Jianping ; Huang, Xingxu ; He, Fuchu ; Zhang, Pumin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5628-8f76c695ebd401c594c8104ed662dbd30dbf640b8f1a922ec14056d4b906447c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenoviridae - genetics</topic><topic>Adenoviruses</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Clustered Regularly Interspaced Short Palindromic Repeats - genetics</topic><topic>CRISPR/Cas9</topic><topic>Gene editing</topic><topic>Gene Targeting - methods</topic><topic>Humans</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Ranran</creatorcontrib><creatorcontrib>Peng, Jin</creatorcontrib><creatorcontrib>Yan, Yonghong</creatorcontrib><creatorcontrib>Cao, Peili</creatorcontrib><creatorcontrib>Wang, Jiewei</creatorcontrib><creatorcontrib>Qiu, Chen</creatorcontrib><creatorcontrib>Tang, Lichun</creatorcontrib><creatorcontrib>Liu, Di</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><creatorcontrib>Jin, Jianping</creatorcontrib><creatorcontrib>Huang, Xingxu</creatorcontrib><creatorcontrib>He, Fuchu</creatorcontrib><creatorcontrib>Zhang, Pumin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Ranran</au><au>Peng, Jin</au><au>Yan, Yonghong</au><au>Cao, Peili</au><au>Wang, Jiewei</au><au>Qiu, Chen</au><au>Tang, Lichun</au><au>Liu, Di</au><au>Tang, Li</au><au>Jin, Jianping</au><au>Huang, Xingxu</au><au>He, Fuchu</au><au>Zhang, Pumin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-11-03</date><risdate>2014</risdate><volume>588</volume><issue>21</issue><spage>3954</spage><epage>3958</epage><pages>3954-3958</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Adenoviral delivery of CRISPR/Cas9 produces efficient gene editing in cultured cells.•Effective gene knockout in the adult mouse liver via adenoviral delivery of CRISPR/Cas9.•The gene editing in the liver is stable over long term and after extensive liver tissue regeneration.
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25241167</pmid><doi>10.1016/j.febslet.2014.09.008</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1846-938X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenoviridae - genetics Adenoviruses Animals Base Sequence Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR/Cas9 Gene editing Gene Targeting - methods Humans Liver Liver - metabolism Male Mice |
| title | Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9 |
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