Spiroketals from Marine Isolates of the Fungi Penicillium thomii KMM 4645 and P. lividum KMM 4663

Recent research showed that micromycete fungi isolated from marine sources are interesting as producers of biologically active compounds [1-3]. In continuation of work on the discovery of biologically active compounds in extracts of marine isolates of micromycete fungi, we isolated the two strains Penicillium lividum and P. thomii from the surface of Sargassum miyabei (collected in Lazurnaya Bay, Peter the Great Gulf, Sea of Japan). Work with the fungi was carried out in parallel. Each fungus was cultivated on standard solid medium. The biomass was worked up with EtOAc followed by separation of the dry residue over silica gel using eluent of increasing polarity. The resulting fractions were separated by reversed-phase HPLC to afford from P. lividum compounds 1 (5 mg) and 2 (38 mg); from P. thomii, 1 (2 mg) and 2 (30 mg). A comparison of the mass spectrometric and PMR and super(13)C NMR spectroscopic data for 1 with the literature identified its structure as that of daldinin D, which was obtain ...