Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes
Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20ψ from Planktothrix rubescens, and inserted it into an expression...
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| Veröffentlicht in: | Journal of Ocean University of China 2015-02, Vol.14 (1), p.84-88 |
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| creator | Wang, Tianhe Kang, Li Li, Jiaheng Wu, Wenjie Zhang, Peiran Gong, Minghao Lai, Weihong Zhang, Chunyan Chang, Lei Peng, Yong Yang, Zhongzhou Li, Lian Bao, Yingying Xu, Haowen Zhang, Xiaohua Sui, Zhenghong Yang, Guanpin Wang, Xianghong |
| description | Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvpA and gvpC20ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvpA and gvpC20ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their ceils. |
| doi_str_mv | 10.1007/s11802-015-2344-3 |
| format | Article |
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They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvpA and gvpC20ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvpA and gvpC20ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their ceils.</description><identifier>ISSN: 1672-5182</identifier><identifier>EISSN: 1993-5021</identifier><identifier>EISSN: 1672-5174</identifier><identifier>DOI: 10.1007/s11802-015-2344-3</identifier><language>eng</language><publisher>Heidelberg: Science Press</publisher><subject>Bacteria ; Cells ; E coli ; Earth and Environmental Science ; Earth Sciences ; Escherichia coli ; Gene expression ; Marine ; Meteorology ; Oceanography ; Planktothrix rubescens ; Proteins ; 囊泡 ; 基因簇 ; 大肠杆菌 ; 气体 ; 浮动 ; 细菌细胞 ; 蓝藻 ; 过表达</subject><ispartof>Journal of Ocean University of China, 2015-02, Vol.14 (1), p.84-88</ispartof><rights>Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg 2014</rights><rights>Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-17ac6a8b70c7c5abe53631f812743167d512f8ab33beb6c5a6cb184c45f5f3b03</citedby><cites>FETCH-LOGICAL-c376t-17ac6a8b70c7c5abe53631f812743167d512f8ab33beb6c5a6cb184c45f5f3b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/87473A/87473A.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11802-015-2344-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11802-015-2344-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Wang, Tianhe</creatorcontrib><creatorcontrib>Kang, Li</creatorcontrib><creatorcontrib>Li, Jiaheng</creatorcontrib><creatorcontrib>Wu, Wenjie</creatorcontrib><creatorcontrib>Zhang, Peiran</creatorcontrib><creatorcontrib>Gong, Minghao</creatorcontrib><creatorcontrib>Lai, Weihong</creatorcontrib><creatorcontrib>Zhang, Chunyan</creatorcontrib><creatorcontrib>Chang, Lei</creatorcontrib><creatorcontrib>Peng, Yong</creatorcontrib><creatorcontrib>Yang, Zhongzhou</creatorcontrib><creatorcontrib>Li, Lian</creatorcontrib><creatorcontrib>Bao, Yingying</creatorcontrib><creatorcontrib>Xu, Haowen</creatorcontrib><creatorcontrib>Zhang, Xiaohua</creatorcontrib><creatorcontrib>Sui, Zhenghong</creatorcontrib><creatorcontrib>Yang, Guanpin</creatorcontrib><creatorcontrib>Wang, Xianghong</creatorcontrib><title>Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes</title><title>Journal of Ocean University of China</title><addtitle>J. Ocean Univ. China</addtitle><addtitle>Journal of Ocean University of China</addtitle><description>Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvpA and gvpC20ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvpA and gvpC20ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their ceils.</description><subject>Bacteria</subject><subject>Cells</subject><subject>E coli</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Escherichia coli</subject><subject>Gene expression</subject><subject>Marine</subject><subject>Meteorology</subject><subject>Oceanography</subject><subject>Planktothrix rubescens</subject><subject>Proteins</subject><subject>囊泡</subject><subject>基因簇</subject><subject>大肠杆菌</subject><subject>气体</subject><subject>浮动</subject><subject>细菌细胞</subject><subject>蓝藻</subject><subject>过表达</subject><issn>1672-5182</issn><issn>1993-5021</issn><issn>1672-5174</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LAzEQhoMoWKs_wNuiFy_RTD63Ryn9EApe1GtIYrbdst20yRbsvzdli4gHL5nAPO-8My9Ct0AegRD1lABKQjEBgSnjHLMzNIDRiGFBKJznv1QUCyjpJbpKaU2IYEKqAZpPm2C6ul0Wk-RWPtZuVZvChaYu7KGYfG2jT-nYHh9MG6xxXWZMU8xMKj58ql3ji5lvfbpGF5Vpkr851SF6n07exnO8eJ29jJ8X2DElOwzKOGlKq4hTThjrBZMMqhKo4iwv-SmAVqWxjFlvZSaks1Byx0UlKmYJG6KHfu42ht3ep05v6uR805jWh33SIAWXKp8OGb3_g67DPrZ5u0xxkZ1pfoYIesrFkFL0ld7GemPiQQPRx2x1n63O2epjtpplDe01KbPt0sdfk_8R3Z2MVqFd7rLux0lKzgSAoOwbDxmGEw</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Wang, Tianhe</creator><creator>Kang, 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| subjects | Bacteria Cells E coli Earth and Environmental Science Earth Sciences Escherichia coli Gene expression Marine Meteorology Oceanography Planktothrix rubescens Proteins 囊泡 基因簇 大肠杆菌 气体 浮动 细菌细胞 蓝藻 过表达 |
| title | Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes |
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