Immunohistochemical Expression of Ionized Calcium Binding Adapter Molecule 1 in Cutaneous Histiocytic Proliferative, Neoplastic and Inflammatory Disorders of Dogs and Cats
Ionized calcium-binding adapter molecule 1 (Iba1) has been used widely as a marker for microglial cells and, recently, was also recognized as a ‘pan-macrophage marker’, as it is expressed by all subpopulations of cells of the monocyte/macrophage lineage. To determine the specificity of Iba1 as an im...
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| Veröffentlicht in: | Journal of comparative pathology 2014-11, Vol.151 (4), p.347-351 |
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| creator | Pierezan, F. Mansell, J. Ambrus, A. Hoffmann, A. Rodrigues |
| description | Ionized calcium-binding adapter molecule 1 (Iba1) has been used widely as a marker for microglial cells and, recently, was also recognized as a ‘pan-macrophage marker’, as it is expressed by all subpopulations of cells of the monocyte/macrophage lineage. To determine the specificity of Iba1 as an immunohistochemical marker for canine and feline histiocytic proliferative, neoplastic and inflammatory disorders of the skin, we evaluated its expression in two types of histiocytic tumours, two non-neoplastic histiocytic proliferative conditions, one case of granulomatous dermatitis and four non-histiocytic tumours. Cells of the monocyte/macrophage lineage in all cases of canine cutaneous histiocytoma (9/9), reactive histiocytosis (9/9), histiocytic sarcomas (5/5), feline progressive dendritic cell histiocytosis (3/3) and macrophages in cutaneous mycobacteriosis (7/7) showed strong cytoplasmic expression of Iba1. Neoplastic cells of melanomas (10/10), lymphomas (7/7), mast cell tumours (7/7) and plasmacytomas (4/4) did not express Iba1. Iba1 is therefore a useful marker of cells of the monocyte/macrophage lineage in canine and feline inflammatory, proliferative and neoplastic conditions and can be used to identify these cells in formalin-fixed, paraffin wax-embedded tissues. Iba1 is not able to differentiate between macrophages and dendritic antigen presenting cells and expression does not allow classification of these histiocytic disorders. |
| doi_str_mv | 10.1016/j.jcpa.2014.07.003 |
| format | Article |
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Cells of the monocyte/macrophage lineage in all cases of canine cutaneous histiocytoma (9/9), reactive histiocytosis (9/9), histiocytic sarcomas (5/5), feline progressive dendritic cell histiocytosis (3/3) and macrophages in cutaneous mycobacteriosis (7/7) showed strong cytoplasmic expression of Iba1. Neoplastic cells of melanomas (10/10), lymphomas (7/7), mast cell tumours (7/7) and plasmacytomas (4/4) did not express Iba1. Iba1 is therefore a useful marker of cells of the monocyte/macrophage lineage in canine and feline inflammatory, proliferative and neoplastic conditions and can be used to identify these cells in formalin-fixed, paraffin wax-embedded tissues. Iba1 is not able to differentiate between macrophages and dendritic antigen presenting cells and expression does not allow classification of these histiocytic disorders.</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1016/j.jcpa.2014.07.003</identifier><identifier>PMID: 25172051</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biomarkers - analysis ; Calcium-Binding Proteins - biosynthesis ; Cat Diseases - diagnosis ; Cats ; Dendritic Cells - metabolism ; Dendritic Cells - pathology ; Dog Diseases - diagnosis ; Dogs ; histiocytic disorder ; Histiocytic Sarcoma - diagnosis ; Immunohistochemistry ; ionized calcium-binding adapter molecule 1 ; Macrophages - metabolism ; Macrophages - pathology ; monocyte/macrophage ; Neoplasms - chemistry ; Neoplasms - metabolism ; Skin Diseases - diagnosis ; Skin Diseases - veterinary</subject><ispartof>Journal of comparative pathology, 2014-11, Vol.151 (4), p.347-351</ispartof><rights>2014</rights><rights>Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-2721520ebf235541f0c033b6486c1d1f44149c54b0f74a493ef56e048a8741433</citedby><cites>FETCH-LOGICAL-c525t-2721520ebf235541f0c033b6486c1d1f44149c54b0f74a493ef56e048a8741433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcpa.2014.07.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25172051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pierezan, F.</creatorcontrib><creatorcontrib>Mansell, J.</creatorcontrib><creatorcontrib>Ambrus, A.</creatorcontrib><creatorcontrib>Hoffmann, A. Rodrigues</creatorcontrib><title>Immunohistochemical Expression of Ionized Calcium Binding Adapter Molecule 1 in Cutaneous Histiocytic Proliferative, Neoplastic and Inflammatory Disorders of Dogs and Cats</title><title>Journal of comparative pathology</title><addtitle>J Comp Pathol</addtitle><description>Ionized calcium-binding adapter molecule 1 (Iba1) has been used widely as a marker for microglial cells and, recently, was also recognized as a ‘pan-macrophage marker’, as it is expressed by all subpopulations of cells of the monocyte/macrophage lineage. To determine the specificity of Iba1 as an immunohistochemical marker for canine and feline histiocytic proliferative, neoplastic and inflammatory disorders of the skin, we evaluated its expression in two types of histiocytic tumours, two non-neoplastic histiocytic proliferative conditions, one case of granulomatous dermatitis and four non-histiocytic tumours. Cells of the monocyte/macrophage lineage in all cases of canine cutaneous histiocytoma (9/9), reactive histiocytosis (9/9), histiocytic sarcomas (5/5), feline progressive dendritic cell histiocytosis (3/3) and macrophages in cutaneous mycobacteriosis (7/7) showed strong cytoplasmic expression of Iba1. Neoplastic cells of melanomas (10/10), lymphomas (7/7), mast cell tumours (7/7) and plasmacytomas (4/4) did not express Iba1. Iba1 is therefore a useful marker of cells of the monocyte/macrophage lineage in canine and feline inflammatory, proliferative and neoplastic conditions and can be used to identify these cells in formalin-fixed, paraffin wax-embedded tissues. Iba1 is not able to differentiate between macrophages and dendritic antigen presenting cells and expression does not allow classification of these histiocytic disorders.</description><subject>Animals</subject><subject>Biomarkers - analysis</subject><subject>Calcium-Binding Proteins - biosynthesis</subject><subject>Cat Diseases - diagnosis</subject><subject>Cats</subject><subject>Dendritic Cells - metabolism</subject><subject>Dendritic Cells - pathology</subject><subject>Dog Diseases - diagnosis</subject><subject>Dogs</subject><subject>histiocytic disorder</subject><subject>Histiocytic Sarcoma - diagnosis</subject><subject>Immunohistochemistry</subject><subject>ionized calcium-binding adapter molecule 1</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - pathology</subject><subject>monocyte/macrophage</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - metabolism</subject><subject>Skin Diseases - diagnosis</subject><subject>Skin Diseases - veterinary</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCH-CAfORAwtix8yFxKWlLVyofBzhbXmfcepXEwXYqlr_EnyRhC0fEYeTDPPOOxg8hLxjkDFj5Zp_vzaRzDkzkUOUAxSOyYbLgWcF485hsADjLmqaSJ-Q0xj0A1LXkT8kJl6ziINmG_NwOwzz6OxeTN3c4OKN7evl9Chij8yP1lm796H5gR1vdGzcP9J0bOzfe0vNOTwkD_eB7NHOPlFE30nZOekQ_R3q9ZDpvDskZ-jn43lkMOrl7fE0_op96HdeOHju6HW2vh0EnHw70wkUfOgxx3X3hb-NvpNUpPiNPrO4jPn94z8jXq8sv7XV28-n9tj2_yYzkMmW84kxywJ3lhZSCWTBQFLtS1KVhHbNCMNEYKXZgK6FFU6CVJYKodV0traI4I6-OuVPw32aMSQ0uGuz742GKlVKUS0HzHyhvZF2WIBaUH1ETfIwBrZqCG3Q4KAZq9an2avWpVp8KKrX4XIZePuTPuwG7vyN_BC7A2yOAy4fcOwwqGoejwc4FNEl13v0r_xePdbKl</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Pierezan, F.</creator><creator>Mansell, J.</creator><creator>Ambrus, A.</creator><creator>Hoffmann, A. 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To determine the specificity of Iba1 as an immunohistochemical marker for canine and feline histiocytic proliferative, neoplastic and inflammatory disorders of the skin, we evaluated its expression in two types of histiocytic tumours, two non-neoplastic histiocytic proliferative conditions, one case of granulomatous dermatitis and four non-histiocytic tumours. Cells of the monocyte/macrophage lineage in all cases of canine cutaneous histiocytoma (9/9), reactive histiocytosis (9/9), histiocytic sarcomas (5/5), feline progressive dendritic cell histiocytosis (3/3) and macrophages in cutaneous mycobacteriosis (7/7) showed strong cytoplasmic expression of Iba1. Neoplastic cells of melanomas (10/10), lymphomas (7/7), mast cell tumours (7/7) and plasmacytomas (4/4) did not express Iba1. 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| subjects | Animals Biomarkers - analysis Calcium-Binding Proteins - biosynthesis Cat Diseases - diagnosis Cats Dendritic Cells - metabolism Dendritic Cells - pathology Dog Diseases - diagnosis Dogs histiocytic disorder Histiocytic Sarcoma - diagnosis Immunohistochemistry ionized calcium-binding adapter molecule 1 Macrophages - metabolism Macrophages - pathology monocyte/macrophage Neoplasms - chemistry Neoplasms - metabolism Skin Diseases - diagnosis Skin Diseases - veterinary |
| title | Immunohistochemical Expression of Ionized Calcium Binding Adapter Molecule 1 in Cutaneous Histiocytic Proliferative, Neoplastic and Inflammatory Disorders of Dogs and Cats |
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