Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary
The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthaniz...
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| Veröffentlicht in: | Ticks and tick-borne diseases 2015-03, Vol.6 (2), p.111-116 |
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| creator | Szekeres, Sándor Claudia Coipan, Elena Rigó, Krisztina Majoros, Gábor Jahfari, Setareh Sprong, Hein Földvári, Gábor |
| description | The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p |
| doi_str_mv | 10.1016/j.ttbdis.2014.10.004 |
| format | Article |
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We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary.</description><identifier>ISSN: 1877-959X</identifier><identifier>EISSN: 1877-9603</identifier><identifier>DOI: 10.1016/j.ttbdis.2014.10.004</identifier><identifier>PMID: 25468763</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Anaplasma phagocytophilum - genetics ; Anaplasma phagocytophilum - isolation & purification ; Anaplasmataceae - genetics ; Anaplasmataceae - isolation & purification ; Anaplasmataceae Infections - epidemiology ; Anaplasmataceae Infections - microbiology ; Anaplasmataceae Infections - veterinary ; Animals ; Arachnid Vectors - microbiology ; DNA, Bacterial - genetics ; Ehrlichiosis - epidemiology ; Ehrlichiosis - microbiology ; Ehrlichiosis - veterinary ; Female ; Humans ; Hungary - epidemiology ; Ixodidae - microbiology ; Male ; Prevalence ; Rodentia</subject><ispartof>Ticks and tick-borne diseases, 2015-03, Vol.6 (2), p.111-116</ispartof><rights>Copyright © 2014 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c307t-82219fba9272b68f06f2b1ecb1fc936d00d592c9881d91876f25d9643d41ef533</citedby><cites>FETCH-LOGICAL-c307t-82219fba9272b68f06f2b1ecb1fc936d00d592c9881d91876f25d9643d41ef533</cites><orcidid>0000-0001-5297-9036</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25468763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Szekeres, Sándor</creatorcontrib><creatorcontrib>Claudia Coipan, Elena</creatorcontrib><creatorcontrib>Rigó, Krisztina</creatorcontrib><creatorcontrib>Majoros, Gábor</creatorcontrib><creatorcontrib>Jahfari, Setareh</creatorcontrib><creatorcontrib>Sprong, Hein</creatorcontrib><creatorcontrib>Földvári, Gábor</creatorcontrib><title>Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary</title><title>Ticks and tick-borne diseases</title><addtitle>Ticks Tick Borne Dis</addtitle><description>The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary.</description><subject>Anaplasma phagocytophilum - genetics</subject><subject>Anaplasma phagocytophilum - isolation & purification</subject><subject>Anaplasmataceae - genetics</subject><subject>Anaplasmataceae - isolation & purification</subject><subject>Anaplasmataceae Infections - epidemiology</subject><subject>Anaplasmataceae Infections - microbiology</subject><subject>Anaplasmataceae Infections - veterinary</subject><subject>Animals</subject><subject>Arachnid Vectors - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>Ehrlichiosis - epidemiology</subject><subject>Ehrlichiosis - microbiology</subject><subject>Ehrlichiosis - veterinary</subject><subject>Female</subject><subject>Humans</subject><subject>Hungary - epidemiology</subject><subject>Ixodidae - microbiology</subject><subject>Male</subject><subject>Prevalence</subject><subject>Rodentia</subject><issn>1877-959X</issn><issn>1877-9603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOxCAUhonRqBl9A2NYupmRS0vL0ky8JUYXauKuoUCnjC1ULgvjy0sdlQ3k8P1wzgfAGUYrjDC73K5ibJUJK4JwkUsrhIo9cIzrqlpyhuj-37nkb0fgNIQtyovioq7IITgiZcHqitFj8LUWVhklYgrwUTvd-8HI3gg4mvfktQ0mwEzAKyumQYRRwKkXGyc_o5t6M6QRGgttjnsxQO-UtvGHj0a-Q-nGMVkTjQ4z9uxS7LW38C7ZjfCfJ-CgE0PQp7_7ArzeXL-s75YPT7f366uHpaSoisuaEMy7VnBSkZbVHWIdabGWLe4kp0whpEpOJK9rrHieOl-XirOCqgLrrqR0AS52707efSQdYjOaIPUwCKtdCg1mJcWkLtiMFjtUeheC110zeTPmXhuMmtl8s2125pvZ_FzN5nPs_PeH1I5a_Yf-PNNvqTqDiA</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Szekeres, Sándor</creator><creator>Claudia Coipan, Elena</creator><creator>Rigó, Krisztina</creator><creator>Majoros, Gábor</creator><creator>Jahfari, Setareh</creator><creator>Sprong, Hein</creator><creator>Földvári, Gábor</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5297-9036</orcidid></search><sort><creationdate>20150301</creationdate><title>Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary</title><author>Szekeres, Sándor ; Claudia Coipan, Elena ; Rigó, Krisztina ; Majoros, Gábor ; Jahfari, Setareh ; Sprong, Hein ; Földvári, Gábor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-82219fba9272b68f06f2b1ecb1fc936d00d592c9881d91876f25d9643d41ef533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anaplasma phagocytophilum - genetics</topic><topic>Anaplasma phagocytophilum - isolation & purification</topic><topic>Anaplasmataceae - genetics</topic><topic>Anaplasmataceae - isolation & purification</topic><topic>Anaplasmataceae Infections - epidemiology</topic><topic>Anaplasmataceae Infections - microbiology</topic><topic>Anaplasmataceae Infections - veterinary</topic><topic>Animals</topic><topic>Arachnid Vectors - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>Ehrlichiosis - epidemiology</topic><topic>Ehrlichiosis - microbiology</topic><topic>Ehrlichiosis - veterinary</topic><topic>Female</topic><topic>Humans</topic><topic>Hungary - epidemiology</topic><topic>Ixodidae - microbiology</topic><topic>Male</topic><topic>Prevalence</topic><topic>Rodentia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szekeres, Sándor</creatorcontrib><creatorcontrib>Claudia Coipan, Elena</creatorcontrib><creatorcontrib>Rigó, Krisztina</creatorcontrib><creatorcontrib>Majoros, Gábor</creatorcontrib><creatorcontrib>Jahfari, Setareh</creatorcontrib><creatorcontrib>Sprong, Hein</creatorcontrib><creatorcontrib>Földvári, Gábor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ticks and tick-borne diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szekeres, Sándor</au><au>Claudia Coipan, Elena</au><au>Rigó, Krisztina</au><au>Majoros, Gábor</au><au>Jahfari, Setareh</au><au>Sprong, Hein</au><au>Földvári, Gábor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary</atitle><jtitle>Ticks and tick-borne diseases</jtitle><addtitle>Ticks Tick Borne Dis</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>6</volume><issue>2</issue><spage>111</spage><epage>116</epage><pages>111-116</pages><issn>1877-959X</issn><eissn>1877-9603</eissn><abstract>The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary.</abstract><cop>Netherlands</cop><pmid>25468763</pmid><doi>10.1016/j.ttbdis.2014.10.004</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5297-9036</orcidid></addata></record> |
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| subjects | Anaplasma phagocytophilum - genetics Anaplasma phagocytophilum - isolation & purification Anaplasmataceae - genetics Anaplasmataceae - isolation & purification Anaplasmataceae Infections - epidemiology Anaplasmataceae Infections - microbiology Anaplasmataceae Infections - veterinary Animals Arachnid Vectors - microbiology DNA, Bacterial - genetics Ehrlichiosis - epidemiology Ehrlichiosis - microbiology Ehrlichiosis - veterinary Female Humans Hungary - epidemiology Ixodidae - microbiology Male Prevalence Rodentia |
| title | Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary |
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