Effect of optical clearing agents on optical coherence tomography images of cervical epithelium
Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on O...
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| Veröffentlicht in: | Lasers in medical science 2015-02, Vol.30 (2), p.517-525 |
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| description | Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia. |
| doi_str_mv | 10.1007/s10103-014-1674-5 |
| format | Article |
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Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-014-1674-5</identifier><identifier>PMID: 25503301</identifier><identifier>CODEN: LMSCEZ</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Acetic Acid - chemistry ; Adult ; Cervical cancer ; Cervical Intraepithelial Neoplasia - diagnosis ; Dentistry ; Dimethyl Sulfoxide - chemistry ; Epithelium - pathology ; Female ; Humans ; Lasers ; Medicine ; Medicine & Public Health ; Middle Aged ; Optical Devices ; Optical properties ; Optics ; Original Article ; Photonics ; Polyethylene Glycols - chemistry ; Prospective Studies ; Quantum Optics ; Time Factors ; Tomography ; Tomography, Optical Coherence ; Uterine Cervical Neoplasms - diagnosis</subject><ispartof>Lasers in medical science, 2015-02, Vol.30 (2), p.517-525</ispartof><rights>Springer-Verlag London 2014</rights><rights>Springer-Verlag London 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-6a4941e11933884212a573dbb7f7c2495a75e4bca164c42262ec5f02f3fbf3363</citedby><cites>FETCH-LOGICAL-c405t-6a4941e11933884212a573dbb7f7c2495a75e4bca164c42262ec5f02f3fbf3363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-014-1674-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-014-1674-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25503301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallwas, Julia</creatorcontrib><creatorcontrib>Stanchi, Anna</creatorcontrib><creatorcontrib>Ditsch, Nina</creatorcontrib><creatorcontrib>Schwarz, Theresa</creatorcontrib><creatorcontrib>Dannecker, Christian</creatorcontrib><creatorcontrib>Mueller, Susanna</creatorcontrib><creatorcontrib>Stepp, Herbert</creatorcontrib><creatorcontrib>Mortensen, Uwe</creatorcontrib><title>Effect of optical clearing agents on optical coherence tomography images of cervical epithelium</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia.</description><subject>Acetic Acid - chemistry</subject><subject>Adult</subject><subject>Cervical cancer</subject><subject>Cervical Intraepithelial Neoplasia - diagnosis</subject><subject>Dentistry</subject><subject>Dimethyl Sulfoxide - chemistry</subject><subject>Epithelium - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Lasers</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Optical Devices</subject><subject>Optical properties</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Prospective Studies</subject><subject>Quantum Optics</subject><subject>Time Factors</subject><subject>Tomography</subject><subject>Tomography, Optical Coherence</subject><subject>Uterine Cervical Neoplasms - 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Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallwas, Julia</au><au>Stanchi, Anna</au><au>Ditsch, Nina</au><au>Schwarz, Theresa</au><au>Dannecker, Christian</au><au>Mueller, Susanna</au><au>Stepp, Herbert</au><au>Mortensen, Uwe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of optical clearing agents on optical coherence tomography images of cervical epithelium</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>30</volume><issue>2</issue><spage>517</spage><epage>525</epage><pages>517-525</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><coden>LMSCEZ</coden><abstract>Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia.</abstract><cop>London</cop><pub>Springer London</pub><pmid>25503301</pmid><doi>10.1007/s10103-014-1674-5</doi><tpages>9</tpages></addata></record> |
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| subjects | Acetic Acid - chemistry Adult Cervical cancer Cervical Intraepithelial Neoplasia - diagnosis Dentistry Dimethyl Sulfoxide - chemistry Epithelium - pathology Female Humans Lasers Medicine Medicine & Public Health Middle Aged Optical Devices Optical properties Optics Original Article Photonics Polyethylene Glycols - chemistry Prospective Studies Quantum Optics Time Factors Tomography Tomography, Optical Coherence Uterine Cervical Neoplasms - diagnosis |
| title | Effect of optical clearing agents on optical coherence tomography images of cervical epithelium |
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