Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case
Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet...
Gespeichert in:
| Veröffentlicht in: | Vaccine 1998-05, Vol.16 (9), p.1029-1038 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1038 |
|---|---|
| container_issue | 9 |
| container_start_page | 1029 |
| container_title | Vaccine |
| container_volume | 16 |
| creator | Saikh, Kamal U. Sesno, Julie Brandler, Patricia Ulrich, Robert G. |
| description | Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet provided some degree of protection from a lethal toxin challenge. Cytotoxic T-cell responses dominated DNA immunizations, while specific T-cell proliferation resulted from all vaccines tested. Immune responses to the DNA vaccine exhibited a T-helper type-1 propensity, while polypeptides elicited T-helper type-2 responses. The lower antibody response to the plasmid vaccine was not due to insufficient quantity of C fragment
in vivo but was likely the result of a mode of antigen presentation that was less efficient for supporting antibody production. Collectively, these results suggest that polypeptide or toxoid vaccines are preferable to plasmid-based vaccination for control of diseases caused by tetanus toxin. |
| doi_str_mv | 10.1016/S0264-410X(97)00280-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16529035</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X97002806</els_id><sourcerecordid>16529035</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-a5cbc0a2a3637a51ca725367312fa2dcc3f068c1f097e022a353ea331155cd9b3</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVpSLfb_ISADqW0B6f6sGTrFJb0KxCaQ1LITYzH46DitVNJDum_r5Jd9tqTEO_zzgwPY6dSnEkh7ecboWxd1VLcfXTNJyFUKyr7iq1k2-hKGdm-ZqsD8oa9Tem3EMJo6Y7ZsbOt0sas2P0mEv_yc1N1kKjnj4AYJkp8STQsIx_myB_inAlzmCcO9xCmlHkijJQL3wFmigFGnuenEp3zW8owLWn355kKjWX0O3Y0wJjoZP-u2a9vX28vflRX198vLzZXFdZK5AoMdihAgba6ASMRGmW0bbRUA6geUQ_CtigH4RoSqnBGE2gtpTHYu06v2Yfd3HL1n6Vs99uQkMYRJpqX5KU1yonSWjOzAzHOKUUa_EMMW4h_vRT-WbB_Eeyf7XnX-BfB3pbe6X7B0m2pP7T2Rkv-fp9DQhiHCBOGdMCUrl0tXcHOdxgVGY-Bok8YaELqQyyyfT-H_xzyD5SSmEI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16529035</pqid></control><display><type>article</type><title>Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Saikh, Kamal U. ; Sesno, Julie ; Brandler, Patricia ; Ulrich, Robert G.</creator><creatorcontrib>Saikh, Kamal U. ; Sesno, Julie ; Brandler, Patricia ; Ulrich, Robert G.</creatorcontrib><description>Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet provided some degree of protection from a lethal toxin challenge. Cytotoxic T-cell responses dominated DNA immunizations, while specific T-cell proliferation resulted from all vaccines tested. Immune responses to the DNA vaccine exhibited a T-helper type-1 propensity, while polypeptides elicited T-helper type-2 responses. The lower antibody response to the plasmid vaccine was not due to insufficient quantity of C fragment
in vivo but was likely the result of a mode of antigen presentation that was less efficient for supporting antibody production. Collectively, these results suggest that polypeptide or toxoid vaccines are preferable to plasmid-based vaccination for control of diseases caused by tetanus toxin.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(97)00280-6</identifier><identifier>PMID: 9682355</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Bacterial - biosynthesis ; Antigen Presentation ; Antigens, Bacterial - genetics ; Applied microbiology ; Biological and medical sciences ; Cell Line ; Clostridium tetani - genetics ; Clostridium tetani - immunology ; cytotoxic T-cells ; Cytotoxicity, Immunologic ; DNA vaccines ; Female ; Fundamental and applied biological sciences. Psychology ; Lymphocyte Activation ; Mice ; Mice, Inbred BALB C ; Microbiology ; Peptide Fragments - immunology ; Peptide Fragments - pharmacology ; Plasmids - genetics ; T-Lymphocytes - immunology ; Tetanus - immunology ; Tetanus - prevention & control ; tetanus toxin ; Tetanus Toxin - genetics ; Tetanus Toxin - immunology ; Tetanus Toxin - pharmacology ; Tetanus Toxoid - genetics ; Tetanus Toxoid - immunology ; Tetanus Toxoid - pharmacology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology ; Vaccines, DNA - pharmacology</subject><ispartof>Vaccine, 1998-05, Vol.16 (9), p.1029-1038</ispartof><rights>1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a5cbc0a2a3637a51ca725367312fa2dcc3f068c1f097e022a353ea331155cd9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X97002806$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2349419$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9682355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saikh, Kamal U.</creatorcontrib><creatorcontrib>Sesno, Julie</creatorcontrib><creatorcontrib>Brandler, Patricia</creatorcontrib><creatorcontrib>Ulrich, Robert G.</creatorcontrib><title>Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet provided some degree of protection from a lethal toxin challenge. Cytotoxic T-cell responses dominated DNA immunizations, while specific T-cell proliferation resulted from all vaccines tested. Immune responses to the DNA vaccine exhibited a T-helper type-1 propensity, while polypeptides elicited T-helper type-2 responses. The lower antibody response to the plasmid vaccine was not due to insufficient quantity of C fragment
in vivo but was likely the result of a mode of antigen presentation that was less efficient for supporting antibody production. Collectively, these results suggest that polypeptide or toxoid vaccines are preferable to plasmid-based vaccination for control of diseases caused by tetanus toxin.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Antigen Presentation</subject><subject>Antigens, Bacterial - genetics</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Clostridium tetani - genetics</subject><subject>Clostridium tetani - immunology</subject><subject>cytotoxic T-cells</subject><subject>Cytotoxicity, Immunologic</subject><subject>DNA vaccines</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Peptide Fragments - immunology</subject><subject>Peptide Fragments - pharmacology</subject><subject>Plasmids - genetics</subject><subject>T-Lymphocytes - immunology</subject><subject>Tetanus - immunology</subject><subject>Tetanus - prevention & control</subject><subject>tetanus toxin</subject><subject>Tetanus Toxin - genetics</subject><subject>Tetanus Toxin - immunology</subject><subject>Tetanus Toxin - pharmacology</subject><subject>Tetanus Toxoid - genetics</subject><subject>Tetanus Toxoid - immunology</subject><subject>Tetanus Toxoid - pharmacology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><subject>Vaccines, DNA - pharmacology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSLfb_ISADqW0B6f6sGTrFJb0KxCaQ1LITYzH46DitVNJDum_r5Jd9tqTEO_zzgwPY6dSnEkh7ecboWxd1VLcfXTNJyFUKyr7iq1k2-hKGdm-ZqsD8oa9Tem3EMJo6Y7ZsbOt0sas2P0mEv_yc1N1kKjnj4AYJkp8STQsIx_myB_inAlzmCcO9xCmlHkijJQL3wFmigFGnuenEp3zW8owLWn355kKjWX0O3Y0wJjoZP-u2a9vX28vflRX198vLzZXFdZK5AoMdihAgba6ASMRGmW0bbRUA6geUQ_CtigH4RoSqnBGE2gtpTHYu06v2Yfd3HL1n6Vs99uQkMYRJpqX5KU1yonSWjOzAzHOKUUa_EMMW4h_vRT-WbB_Eeyf7XnX-BfB3pbe6X7B0m2pP7T2Rkv-fp9DQhiHCBOGdMCUrl0tXcHOdxgVGY-Bok8YaELqQyyyfT-H_xzyD5SSmEI</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Saikh, Kamal U.</creator><creator>Sesno, Julie</creator><creator>Brandler, Patricia</creator><creator>Ulrich, Robert G.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>19980501</creationdate><title>Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case</title><author>Saikh, Kamal U. ; Sesno, Julie ; Brandler, Patricia ; Ulrich, Robert G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-a5cbc0a2a3637a51ca725367312fa2dcc3f068c1f097e022a353ea331155cd9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>Antigen Presentation</topic><topic>Antigens, Bacterial - genetics</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Clostridium tetani - genetics</topic><topic>Clostridium tetani - immunology</topic><topic>cytotoxic T-cells</topic><topic>Cytotoxicity, Immunologic</topic><topic>DNA vaccines</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Peptide Fragments - immunology</topic><topic>Peptide Fragments - pharmacology</topic><topic>Plasmids - genetics</topic><topic>T-Lymphocytes - immunology</topic><topic>Tetanus - immunology</topic><topic>Tetanus - prevention & control</topic><topic>tetanus toxin</topic><topic>Tetanus Toxin - genetics</topic><topic>Tetanus Toxin - immunology</topic><topic>Tetanus Toxin - pharmacology</topic><topic>Tetanus Toxoid - genetics</topic><topic>Tetanus Toxoid - immunology</topic><topic>Tetanus Toxoid - pharmacology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><topic>Vaccines, DNA - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saikh, Kamal U.</creatorcontrib><creatorcontrib>Sesno, Julie</creatorcontrib><creatorcontrib>Brandler, Patricia</creatorcontrib><creatorcontrib>Ulrich, Robert G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saikh, Kamal U.</au><au>Sesno, Julie</au><au>Brandler, Patricia</au><au>Ulrich, Robert G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>16</volume><issue>9</issue><spage>1029</spage><epage>1038</epage><pages>1029-1038</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet provided some degree of protection from a lethal toxin challenge. Cytotoxic T-cell responses dominated DNA immunizations, while specific T-cell proliferation resulted from all vaccines tested. Immune responses to the DNA vaccine exhibited a T-helper type-1 propensity, while polypeptides elicited T-helper type-2 responses. The lower antibody response to the plasmid vaccine was not due to insufficient quantity of C fragment
in vivo but was likely the result of a mode of antigen presentation that was less efficient for supporting antibody production. Collectively, these results suggest that polypeptide or toxoid vaccines are preferable to plasmid-based vaccination for control of diseases caused by tetanus toxin.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9682355</pmid><doi>10.1016/S0264-410X(97)00280-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1998-05, Vol.16 (9), p.1029-1038 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16529035 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Antibodies, Bacterial - biosynthesis Antigen Presentation Antigens, Bacterial - genetics Applied microbiology Biological and medical sciences Cell Line Clostridium tetani - genetics Clostridium tetani - immunology cytotoxic T-cells Cytotoxicity, Immunologic DNA vaccines Female Fundamental and applied biological sciences. Psychology Lymphocyte Activation Mice Mice, Inbred BALB C Microbiology Peptide Fragments - immunology Peptide Fragments - pharmacology Plasmids - genetics T-Lymphocytes - immunology Tetanus - immunology Tetanus - prevention & control tetanus toxin Tetanus Toxin - genetics Tetanus Toxin - immunology Tetanus Toxin - pharmacology Tetanus Toxoid - genetics Tetanus Toxoid - immunology Tetanus Toxoid - pharmacology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - genetics Vaccines, DNA - immunology Vaccines, DNA - pharmacology |
| title | Are DNA-based vaccines useful for protection against secreted bacterial toxins? Tetanus toxin test case |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T15%3A38%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Are%20DNA-based%20vaccines%20useful%20for%20protection%20against%20secreted%20bacterial%20toxins?%20Tetanus%20toxin%20test%20case&rft.jtitle=Vaccine&rft.au=Saikh,%20Kamal%20U.&rft.date=1998-05-01&rft.volume=16&rft.issue=9&rft.spage=1029&rft.epage=1038&rft.pages=1029-1038&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/S0264-410X(97)00280-6&rft_dat=%3Cproquest_cross%3E16529035%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16529035&rft_id=info:pmid/9682355&rft_els_id=S0264410X97002806&rfr_iscdi=true |