Expression and cellular localization of the Nef protein from human immunodeficiency virus-1 in stably transfected B-cells

Nef protein, encoded by the regulatory nef gene of human immunodeficiency virus type 1 (HIV-1), was expressed in the B-cell line Raji. The cells were stably transfected with plasmids containing the nef transcriptional cassette. They expressed Nef with an Mr of 27,000; the yield could be augmented by...

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Veröffentlicht in:	Archives of virology 1992-01, Vol.124 (1-2), p.123-132
Hauptverfasser:	KIENZLE, N, BACHMANN, M, MÜLLER, W. E. G, MÜLLER-LANTZCH, N
Format:	Artikel
Sprache:	eng
Schlagworte:	B-Lymphocytes - chemistry B-Lymphocytes - drug effects Biological and medical sciences Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Products, nef - biosynthesis Gene Products, nef - drug effects Gene Products, nef - isolation & purification Genetic Vectors - genetics HIV-1 - genetics HIV-1 - metabolism human immunodeficiency virus 1 Humans Microbiology Microscopy - methods nef Gene Products, Human Immunodeficiency Virus Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Tetradecanoylphorbol Acetate - pharmacology Transfection Tumor Cells, Cultured Virology
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