Molecular markers located proximal to the soybean cyst nematode resistance gene, Rhg4
Probe pBLT24 is a cDNA encoding a 34 kDa soybean seed protein with sequence homology to thiol proteases of the papain family. Probe pBLT65 was isolated from a lambda gt11 cDNA library screened with a carrot cDNA encoding aspartokinase-homoserine dehydrogenase. The two probes used in this study were...
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| Veröffentlicht in: | Theoretical and applied genetics 1992-11, Vol.85 (2-3), p.136-138 |
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| container_end_page | 138 |
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| container_issue | 2-3 |
| container_start_page | 136 |
| container_title | Theoretical and applied genetics |
| container_volume | 85 |
| creator | Weisemann, J.M. (United States Dept. of Agriculture/Agricultural Research Service, Beltsville, MD (USA). Plant Sciences Inst.. Plant Molecular Biology Lab.) Matthews, B.F Devine, T.E |
| description | Probe pBLT24 is a cDNA encoding a 34 kDa soybean seed protein with sequence homology to thiol proteases of the papain family. Probe pBLT65 was isolated from a lambda gt11 cDNA library screened with a carrot cDNA encoding aspartokinase-homoserine dehydrogenase. The two probes used in this study were maintained as inserts in E. coli plasmid vectors. Plasmid DNA was digested with a restriction endonuclease to release the insert. DNA fragments were purified on agarose gels. The DNA fragments to be use as probes were radiolabeled using the random oligonucleotides labeling procedure of Feinberg and Vogelstein. |
| doi_str_mv | 10.1007/BF00222850 |
| format | Article |
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| ispartof | Theoretical and applied genetics, 1992-11, Vol.85 (2-3), p.136-138 |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Biological and medical sciences Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology GENETIC MARKERS Genetics of eukaryotes. Biological and molecular evolution GLYCINE MAX HETERODERA Heterodera glycines MARCADORES GENETICOS MARQUEUR GENETIQUE NUCLEOTIDE SEQUENCE Pteridophyta, spermatophyta RESISTANCE AUX FACTEURS NUISIBLES RESISTANCE TO INJURIOUS FACTORS RESISTENCIA A AGENTES DANINOS SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Vegetals |
| title | Molecular markers located proximal to the soybean cyst nematode resistance gene, Rhg4 |
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