Synthesis of Lysozyme-Metallacarborane Conjugates and the Effect of Boron Cluster Modification on Protein Structure and Function
Two complementary methods, “in solution” and “in solid state”, for the synthesis of lysozyme modified with metallacarborane (cobalt bis(dicarbollide), Co(C2B9H11)22−) were developed. As metallacarborane donors, oxonium adducts of cobalt bis(dicarbollide) and 1,4‐dioxane or tetrahydropyran were used....
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2015-02, Vol.16 (3), p.424-431 |
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| container_title | Chembiochem : a European journal of chemical biology |
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| creator | Kowalski, Konrad Goszczyński, Tomasz Leśnikowski, Zbigniew J. Boratyński, Janusz |
| description | Two complementary methods, “in solution” and “in solid state”, for the synthesis of lysozyme modified with metallacarborane (cobalt bis(dicarbollide), Co(C2B9H11)22−) were developed. As metallacarborane donors, oxonium adducts of cobalt bis(dicarbollide) and 1,4‐dioxane or tetrahydropyran were used. The physicochemical and biochemical properties of the obtained lysozyme–metallacarborane conjugates were studied for changes in secondary and tertiary structure, aggregation behavior, and biological activity. Only minor changes in primary, secondary, and tertiary protein structure were observed, caused by the single substitution of metallacarborane on lysozyme. However, the modification produced significant changes in lysozyme enzymatic activity and a tendency toward time‐ and temperature‐dependent aggregation.
An “in solid‐state”, high temperature, method for protein modification with metallacarborane (3‐cobalt bis(dicarbollide) has been developed. Oxonium adducts of cyclic ether and metallacarborane were used as metallacarborane donors. |
| doi_str_mv | 10.1002/cbic.201402611 |
| format | Article |
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An “in solid‐state”, high temperature, method for protein modification with metallacarborane (3‐cobalt bis(dicarbollide) has been developed. Oxonium adducts of cyclic ether and metallacarborane were used as metallacarborane donors.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.201402611</identifier><identifier>PMID: 25589498</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Boron - chemistry ; boron cluster ; Circular Dichroism ; Cobalt - chemistry ; conjugation ; lysozyme ; metallacarborane ; Models, Molecular ; Muramidase - chemistry ; Muramidase - metabolism ; Organometallic Compounds - chemical synthesis ; Protein Conformation ; Protein Denaturation ; Protein folding ; protein modification ; Protein Stability ; Solid-Phase Synthesis Techniques - methods ; Spectrometry, Mass, Electrospray Ionization</subject><ispartof>Chembiochem : a European journal of chemical biology, 2015-02, Vol.16 (3), p.424-431</ispartof><rights>2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4881-ff2abb9fdd540f660657daa65730b56f802a4d39dc6431156d63be06c83339913</citedby><cites>FETCH-LOGICAL-c4881-ff2abb9fdd540f660657daa65730b56f802a4d39dc6431156d63be06c83339913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbic.201402611$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbic.201402611$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25589498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kowalski, Konrad</creatorcontrib><creatorcontrib>Goszczyński, Tomasz</creatorcontrib><creatorcontrib>Leśnikowski, Zbigniew J.</creatorcontrib><creatorcontrib>Boratyński, Janusz</creatorcontrib><title>Synthesis of Lysozyme-Metallacarborane Conjugates and the Effect of Boron Cluster Modification on Protein Structure and Function</title><title>Chembiochem : a European journal of chemical biology</title><addtitle>ChemBioChem</addtitle><description>Two complementary methods, “in solution” and “in solid state”, for the synthesis of lysozyme modified with metallacarborane (cobalt bis(dicarbollide), Co(C2B9H11)22−) were developed. As metallacarborane donors, oxonium adducts of cobalt bis(dicarbollide) and 1,4‐dioxane or tetrahydropyran were used. The physicochemical and biochemical properties of the obtained lysozyme–metallacarborane conjugates were studied for changes in secondary and tertiary structure, aggregation behavior, and biological activity. Only minor changes in primary, secondary, and tertiary protein structure were observed, caused by the single substitution of metallacarborane on lysozyme. However, the modification produced significant changes in lysozyme enzymatic activity and a tendency toward time‐ and temperature‐dependent aggregation.
An “in solid‐state”, high temperature, method for protein modification with metallacarborane (3‐cobalt bis(dicarbollide) has been developed. Oxonium adducts of cyclic ether and metallacarborane were used as metallacarborane donors.</description><subject>Boron - chemistry</subject><subject>boron cluster</subject><subject>Circular Dichroism</subject><subject>Cobalt - chemistry</subject><subject>conjugation</subject><subject>lysozyme</subject><subject>metallacarborane</subject><subject>Models, Molecular</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - metabolism</subject><subject>Organometallic Compounds - chemical synthesis</subject><subject>Protein Conformation</subject><subject>Protein Denaturation</subject><subject>Protein folding</subject><subject>protein modification</subject><subject>Protein Stability</subject><subject>Solid-Phase Synthesis Techniques - methods</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEchoMotlavHmXAi5dZ8zHJTI526BdstXWVgpeQyYdmnZ20SYZ2PPmnm3HXRbwIIQnheV5-4QXgJYILBCF-qzqnFhiiCmKG0CNwiCrCy5oR8nh3rzCuD8CzGNcQQs4IegoOMKUNr3hzCH6upiF9M9HFwttiOUX_Y9qY8tIk2fdSydD5IAdTtH5Yj19lMrGQgy6yUpxYa1SatWMf_FC0_RiTCcWl1846JZPLj3ldBZ-MG4pVCqNKYzC_E07HQc3Ec_DEyj6aF7vzCHw-PfnUnpfLD2cX7btlqaqmQaW1WHYdt1rTClrGIKO1ljLvBHaU2QZiWWnCtWIVQYgyzUhnIFMNIYRzRI7Am23ubfB3o4lJbFxUJn9yMH6MAjGKK0oJqTP6-h907ccw5OlmClKEGGkytdhSKvgYg7HiNriNDJNAUMzdiLkbse8mC692sWO3MXqP_ykjA3wL3LveTP-JE-3xRft3eLl1Xe7gYe_K8F2wmtRU3Lw_E19urq6v-flKfCS_ABZcqxQ</recordid><startdate>20150209</startdate><enddate>20150209</enddate><creator>Kowalski, Konrad</creator><creator>Goszczyński, Tomasz</creator><creator>Leśnikowski, Zbigniew J.</creator><creator>Boratyński, Janusz</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20150209</creationdate><title>Synthesis of Lysozyme-Metallacarborane Conjugates and the Effect of Boron Cluster Modification on Protein Structure and Function</title><author>Kowalski, Konrad ; Goszczyński, Tomasz ; Leśnikowski, Zbigniew J. ; Boratyński, Janusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4881-ff2abb9fdd540f660657daa65730b56f802a4d39dc6431156d63be06c83339913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Boron - chemistry</topic><topic>boron cluster</topic><topic>Circular Dichroism</topic><topic>Cobalt - chemistry</topic><topic>conjugation</topic><topic>lysozyme</topic><topic>metallacarborane</topic><topic>Models, Molecular</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - metabolism</topic><topic>Organometallic Compounds - chemical synthesis</topic><topic>Protein Conformation</topic><topic>Protein Denaturation</topic><topic>Protein folding</topic><topic>protein modification</topic><topic>Protein Stability</topic><topic>Solid-Phase Synthesis Techniques - methods</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kowalski, Konrad</creatorcontrib><creatorcontrib>Goszczyński, Tomasz</creatorcontrib><creatorcontrib>Leśnikowski, Zbigniew J.</creatorcontrib><creatorcontrib>Boratyński, Janusz</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chembiochem : a European journal of chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kowalski, Konrad</au><au>Goszczyński, Tomasz</au><au>Leśnikowski, Zbigniew J.</au><au>Boratyński, Janusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of Lysozyme-Metallacarborane Conjugates and the Effect of Boron Cluster Modification on Protein Structure and Function</atitle><jtitle>Chembiochem : a European journal of chemical biology</jtitle><addtitle>ChemBioChem</addtitle><date>2015-02-09</date><risdate>2015</risdate><volume>16</volume><issue>3</issue><spage>424</spage><epage>431</epage><pages>424-431</pages><issn>1439-4227</issn><eissn>1439-7633</eissn><abstract>Two complementary methods, “in solution” and “in solid state”, for the synthesis of lysozyme modified with metallacarborane (cobalt bis(dicarbollide), Co(C2B9H11)22−) were developed. As metallacarborane donors, oxonium adducts of cobalt bis(dicarbollide) and 1,4‐dioxane or tetrahydropyran were used. The physicochemical and biochemical properties of the obtained lysozyme–metallacarborane conjugates were studied for changes in secondary and tertiary structure, aggregation behavior, and biological activity. Only minor changes in primary, secondary, and tertiary protein structure were observed, caused by the single substitution of metallacarborane on lysozyme. However, the modification produced significant changes in lysozyme enzymatic activity and a tendency toward time‐ and temperature‐dependent aggregation.
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| subjects | Boron - chemistry boron cluster Circular Dichroism Cobalt - chemistry conjugation lysozyme metallacarborane Models, Molecular Muramidase - chemistry Muramidase - metabolism Organometallic Compounds - chemical synthesis Protein Conformation Protein Denaturation Protein folding protein modification Protein Stability Solid-Phase Synthesis Techniques - methods Spectrometry, Mass, Electrospray Ionization |
| title | Synthesis of Lysozyme-Metallacarborane Conjugates and the Effect of Boron Cluster Modification on Protein Structure and Function |
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